Abstract
Summary Calcineurin B‐like interacting protein kinase (CIPKs) has been shown to be required for biotic stress tolerance of plants in plant‐pathogen interactions. However, the roles of CIPKs in immune signalling of cereal crops and an in‐depth knowledge of substrates of CIPKs in response to biotic stress are under debate. In this study, we identified and cloned a CIPK homologue gene TaCIPK10 from wheat. TaCIPK10 was rapidly induced by Puccinia striiformis f. sp . tritici ( Pst ) inoculation and salicylic acid (SA) treatment. In vitro phosphorylation assay demonstrated that the kinase activity of TaCIPK10 is regulated by Ca 2+ and TaCBL4. Knockdown TaCIPK10 significantly reduced wheat resistance to Pst , whereas TaCIPK10 overexpression resulted in enhanced wheat resistance to Pst by the induction of defense response in different aspects, including hypersensitive cell death, ROS accumulation and pathogenesis‐relative genes expression. Moreover, TaCIPK10 physically interacted with and phosphorylated TaNH2, which was homologous to AtNPR3/4. Silencing of TaNH2 in wheat resulted in enhanced susceptibility to the avirulent Pst race, CYR23, indicating its positive role in wheat resistance. Our results demonstrate that TaCIPK10 positively regulate wheat resistance to Pst as molecular links between of Ca 2+ and downstream components of defense response and TaCIPK10 interacts with and phosphorylates TaNH2 to regulate wheat resistance to Pst .
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Claims 8
Claim · model-written summary
TaCIPK10 was identified and cloned in wheat.
- Taxon
- wheat
Triticum aestivume0 - Gene
- TaCIPK10e0
- Outcome
- identified and clonede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified and cloned a CIPK homologue gene TaCIPK10 from wheat”
Claim · model-written summary
Pst inoculation rapidly induced TaCIPK10.
- Treatment
- Puccinia striiformis f. sp . tritici ( Pst ) inoculatione0
- Interactor
- Puccinia striiformis f. sp . tritici
Puccinia striiformise0pathogen - Gene
- TaCIPK10e0
- Outcome
- TaCIPK10e0
- Direction
- rapidly inducede0
Not reportedTaxonComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCIPK10 was rapidly induced by Puccinia striiformis f. sp . tritici ( Pst ) inoculation”
Claim · model-written summary
Salicylic acid treatment rapidly induced TaCIPK10.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- salicylic acid (SA) treatmente0
- Gene
- TaCIPK10e0
- Outcome
- TaCIPK10e0
- Direction
- rapidly inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCIPK10 was rapidly induced by Puccinia striiformis f. sp . tritici ( Pst ) inoculation and salicylic acid (SA) treatment”
t0“wheat”
Claim · model-written summary
Calcium and TaCBL4 regulated TaCIPK10 kinase activity in vitro.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the kinase activity of TaCIPK10 is regulated by Ca 2+ and TaCBL4”
t0“wheat”
Claim · model-written summary
TaCIPK10 knockdown significantly reduced wheat resistance to Pst.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Knockdown TaCIPK10e0
- Interactor
- Pste0pathogen
- Gene
- TaCIPK10e0
- Outcome
- resistance to Pste0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockdown TaCIPK10 significantly reduced wheat resistance to Pst”
Claim · model-written summary
TaCIPK10 overexpression enhanced wheat resistance to Pst.
- Taxon
- wheatTriticum aestivume0
- Treatment
- TaCIPK10 overexpressione0
- Interactor
- Pste0pathogen
- Gene
- TaCIPK10e0
- Outcome
- resistance to Pste0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCIPK10 overexpression resulted in enhanced wheat resistance to Pst”
Claim · model-written summary
TaCIPK10 interacted with and phosphorylated TaNH2.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaCIPK10e0
- Outcome
- interacted with and phosphorylated TaNH2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCIPK10 physically interacted with and phosphorylated TaNH2”
t0“wheat”
Claim · model-written summary
TaNH2 silencing increased wheat susceptibility to avirulent Pst race CYR23.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Silencing of TaNH2e0
- Interactor
- Pste0pathogen
- Gene
- TaNH2e0
- Outcome
- susceptibilitye0
- Direction
- enhancede0
- Qualifier
- CYR23e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of TaNH2 in wheat resulted in enhanced susceptibility to the avirulent Pst race, CYR23”
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loss of function · disease · Triticum aestivum (wheat)Positive and negative results
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