Abstract
Codiaeum variegatum (L) Blume cv. "Corazon de oro" and cv. "Norma" are successfully micropropagated when culture are initiated with explants taken from newly sprouted shoots. The establishment and multiplication steps are possible when 1 mg/L BA or 1 mg/L IAA and 3 mg/L 2iP are added to MS medium, according to the cultivar respectively selected.Adventive organogenesis and somatic embryogenesis are induced from leaf explants taken from in vitro buds of croton. On leaf-sectioned of "Corazon de oro" cultured in vitro, 1 mg/L BA stimulates continuous somatic embryos development and induces some shoots too. Replacing BA with 1 mg/L TDZ induces up to 100% bud regeneration in the same explants. On the other hand, leaf-sectioned of C. variegatum cv. Norma does not start somatic embryo differentiation if 1 mg/L TDZ is not added to the MS basal medium. Incipient callus is observed after 30 days of culture, and then, subculture to MS with 1 mg/L BA allows the same process to show on the "Corazon de oro" cultivar. Somatic embryos show growth arrest that is partially overcome by transfer to hormone-free basal medium with activated charcoal. Root induction is possible on basal medium plus 1 mg/L IBA. Plantlets in the greenhouse have variegated leaves true-to-type.
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Claims 13
Claim · model-written summary
Both cultivars were micropropagated from newly sprouted shoot explants.
- Taxon
- Codiaeum variegatum
Codiaeum variegatume0 - Organ / stage
- newly sprouted shootse0
- Outcome
- successfully micropropagatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Codiaeum variegatum (L) Blume cv. "Corazon de oro" and cv. "Norma" are successfully micropropagated when culture are initiated with explants taken from newly sprouted shoots”
Claim · model-written summary
The establishment and multiplication steps were possible with BA in the selected cultivar.
Not reportedTaxonComparatorDirection
Verbatim
e0“The establishment and multiplication steps are possible when 1 mg/L BA or 1 mg/L IAA and 3 mg/L 2iP are added to MS medium, according to the cultivar respectively selected”
Claim · model-written summary
The establishment and multiplication steps were possible with IAA and 2iP in the selected cultivar.
Not reportedTaxonComparatorDirection
Verbatim
e0“The establishment and multiplication steps are possible when 1 mg/L BA or 1 mg/L IAA and 3 mg/L 2iP are added to MS medium, according to the cultivar respectively selected”
Claim · model-written summary
Adventitious organogenesis and somatic embryogenesis were induced from croton leaf explants.
- Taxon
- crotonCodiaeum variegatume0
- Organ / stage
- leaf explantse0
- Outcome
- Adventive organogenesis and somatic embryogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adventive organogenesis and somatic embryogenesis are induced from leaf explants taken from in vitro buds of croton”
Claim · model-written summary
BA stimulated continuous somatic embryo development in Corazon de oro leaf sections.
- Material
- Corazon de oroe0
- Treatment
- 1 mg/L BAe0
- Outcome
- somatic embryos developmente0
- Direction
- stimulatese0
Not reportedTaxonComparator
Verbatim
e0“On leaf-sectioned of "Corazon de oro" cultured in vitro, 1 mg/L BA stimulates continuous somatic embryos development”
Claim · model-written summary
BA also induced shoots in Corazon de oro leaf sections.
Not reportedTaxonComparator
Verbatim
e0“On leaf-sectioned of "Corazon de oro" cultured in vitro, 1 mg/L BA stimulates continuous somatic embryos development and induces some shoots too”
Claim · model-written summary
Replacing BA with TDZ induced up to 100% bud regeneration.
Not reportedTaxon
Verbatim
e0“Replacing BA with 1 mg/L TDZ induces up to 100% bud regeneration in the same explants”
Claim · model-written summary
Norma leaf sections required TDZ to initiate somatic embryo differentiation.
- Taxon
- C. variegatumCodiaeum variegatume0
- Material
- Normae0
- Treatment
- 1 mg/L TDZe0
- Outcome
- somatic embryo differentiatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“leaf-sectioned of C. variegatum cv. Norma does not start somatic embryo differentiation if 1 mg/L TDZ is not added to the MS basal medium”
Claim · model-written summary
Incipient callus was observed after 30 days of culture.
Not reportedTaxon
Verbatim
e0“Incipient callus is observed after 30 days of culture”
Claim · model-written summary
Subculture onto BA medium enabled the same process as in Corazon de oro.
Not reportedTaxonComparatorDirection
Verbatim
e0“subculture to MS with 1 mg/L BA allows the same process to show on the "Corazon de oro" cultivar”
Claim · model-written summary
Activated charcoal in hormone-free medium partially overcame somatic embryo growth arrest.
- Treatment
- hormone-free basal medium with activated charcoale0
- Outcome
- growth arreste0
- Direction
- partially overcomee0
Not reportedTaxonComparator
Verbatim
e0“Somatic embryos show growth arrest that is partially overcome by transfer to hormone-free basal medium with activated charcoal”
Claim · model-written summary
IBA in basal medium enabled root induction.
Not reportedTaxonComparatorDirection
Verbatim
e0“Root induction is possible on basal medium plus 1 mg/L IBA”
Claim · model-written summary
Greenhouse plantlets had true-to-type variegated leaves.
Not reportedTaxon
Verbatim
e0“Plantlets in the greenhouse have variegated leaves true-to-type”
The same questions in other papers
N-benzyladenine · growth · Codiaeum variegatum (garden croton)Results agree
1 paper in the same direction as this paper
- positive
in vitro · growth · Codiaeum variegatum (garden croton)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.