Abstract
Abstract Anthracnose, caused by Colletotrichum musae, is the most devastating postharvest disease limiting the shelf life and marketability of banana fruit. While γ-aminobutyric acid (GABA) is known to mitigate abiotic stress, its role and mechanisms in controlling postharvest diseases remain elusive. Here, we report that exogenous GABA treatment significantly attenuates anthracnose severity in harvested banana fruit. Through integrative physiological and transcriptomic analyses, we identified a non-specific lipid transfer protein, MaLTP60, as a critical regulator robustly triggered by GABA signaling and C. musae challenge. Crucially, silencing of MaLTP60 attenuated GABA-induced disease resistance. Then, we demonstrate that MaLTP60 orchestrates a dual-mechanism antioxidant defense to maintain redox homeostasis. Mechanistically, MaLTP60 physically targets the mitochondrial voltage-dependent anion channel MaVDAC9. Structural modeling and functional assays reveal that MaLTP60 acts as a molecular plug to occlude the MaVDAC9 pore, thereby blocking ROS transport, inhibiting channel activity and antagonizing MaVDAC9-mediated susceptibility. Concurrently, MaLTP60 interacts with the peroxiredoxin MaPRDX1 and functions as an affinity helper, synergistically enhancing its stabilization and ROS-scavenging efficiency. Collectively, our findings uncover a novel regulatory network in which GABA-induced MaLTP60 functions as a redox brake that coordinates dual antioxidant strategies to confer basal immunity in banana fruit to C. musae.
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Claims 10
Claim · model-written summary
Exogenous GABA significantly reduced anthracnose severity in harvested banana fruit.
- Taxon
- bananae0
- Treatment
- exogenous GABA treatmente0
- Interactor
- Colletotrichum musae
-_Anthracnose_caused_by_Colletotrichum_musae.jpg?width=60)
Colletotrichum musaee1pathogen - Outcome
- anthracnose severitye0
- Direction
- attenuatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
-_Anthracnose_caused_by_Colletotrichum_musae.jpg?width=500)
Verbatim
e0“exogenous GABA treatment significantly attenuates anthracnose severity in harvested banana fruit.”
e1“Anthracnose, caused by Colletotrichum musae”
Claim · model-written summary
GABA signaling and C. musae challenge robustly triggered MaLTP60.
- Taxon
- bananae1
- Interactor
- C. musaeColletotrichum musaee0pathogen
- Gene
- MaLTP60e0
- Outcome
- robustly triggerede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MaLTP60, as a critical regulator robustly triggered by GABA signaling and C. musae challenge.”
e1“harvested banana fruit.”
Claim · model-written summary
Silencing MaLTP60 attenuated GABA-induced banana disease resistance.
- Taxon
- bananae1
- Treatment
- silencing of MaLTP60e0
- Gene
- MaLTP60e0
- Outcome
- GABA-induced disease resistancee0
- Direction
- attenuatede0
Not reportedComparator
Verbatim
e0“silencing of MaLTP60 attenuated GABA-induced disease resistance.”
e1“harvested banana fruit.”
Claim · model-written summary
MaLTP60 was reported to maintain redox homeostasis through dual antioxidant defense.
Verbatim
e0“MaLTP60 orchestrates a dual-mechanism antioxidant defense to maintain redox homeostasis.”
e1“harvested banana fruit.”
Claim · model-written summary
MaLTP60 physically targets MaVDAC9.
Verbatim
e0“MaLTP60 physically targets the mitochondrial voltage-dependent anion channel MaVDAC9.”
e1“harvested banana fruit.”
Claim · model-written summary
MaLTP60 occludes the MaVDAC9 pore, blocking ROS transport.
Verbatim
e0“MaLTP60 acts as a molecular plug to occlude the MaVDAC9 pore, thereby blocking ROS transport”
e1“harvested banana fruit.”
Claim · model-written summary
MaLTP60 inhibits MaVDAC9 channel activity.
Verbatim
e0“MaLTP60 acts as a molecular plug to occlude the MaVDAC9 pore, thereby blocking ROS transport, inhibiting channel activity and antagonizing MaVDAC9-mediated susceptibility.”
e1“harvested banana fruit.”
Claim · model-written summary
MaLTP60 antagonizes MaVDAC9-mediated susceptibility.
Verbatim
e0“antagonizing MaVDAC9-mediated susceptibility.”
e1“MaLTP60 acts as a molecular plug”
e2“harvested banana fruit.”
Claim · model-written summary
MaLTP60 interacts with MaPRDX1 and enhances its stabilization.
Verbatim
e0“MaLTP60 interacts with the peroxiredoxin MaPRDX1 and functions as an affinity helper, synergistically enhancing its stabilization and ROS-scavenging efficiency.”
e1“harvested banana fruit.”
Claim · model-written summary
MaLTP60 enhances MaPRDX1 ROS-scavenging efficiency.
Verbatim
e0“MaLTP60 interacts with the peroxiredoxin MaPRDX1 and functions as an affinity helper, synergistically enhancing its stabilization and ROS-scavenging efficiency.”
e1“harvested banana fruit.”
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