Abstract
Banana is sensitive to cold stress and often suffers from chilling injury with browning peel and failure to normal ripening. We have previously reported that banana chilling injury is accompanied by a reduction of miR528 accumulation, alleviating the degradation of its target gene MaPPO and raising ROS levels that cause peel browning. Here, we further revealed that the miR528-MaPPO cold-responsive module was regulated by miR156-targeted SPL transcription factors, and the miR156c-MaSPL4 module was also responsive to cold stress in banana. Transient overexpression of miR156c resulted in a more severe chilling phenotype by decreasing the expression of MaSPL4 and miR528. Conversely, the browning was alleviated in STTM-miR156c silencing and OE-MaSPL4 samples. Furthermore, DNA affinity purification sequencing and MaSPL4-overexpressing transcriptome jointly revealed that MaSPL4 may mediate the transcription of genes related to lipid metabolism and antioxidation, in addition to the miR528-MaPPO module, demonstrating MaSPL4 as a master regulator in the fruit cold response network. In summary, our results suggest that the miR156c-MaSPL4 module can mediate the chilling response in banana by regulating the miR528-MaPPO module and multiple other pathways, which provides evidence for the crosstalk between TFs and miRNAs that can be used for the molecular breeding of fruit cold tolerance.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Transient overexpression of miR156c in banana resulted in a more severe chilling phenotype.
- Taxon
- Bananae0
- Treatment
- Transient overexpression of miR156ce1
- Outcome
- chilling phenotypee1
- Direction
- more severee1
Not reportedComparator
Verbatim
e0“Banana is sensitive to cold stress and often suffers from chilling injury”
e1“Transient overexpression of miR156c resulted in a more severe chilling phenotype”
Claim · model-written summary
Transient overexpression of miR156c decreased the expression of MaSPL4 and miR528 in banana.
- Taxon
- Bananae0
- Treatment
- Transient overexpression of miR156ce1
- Outcome
- expression of MaSPL4 and miR528e2
- Direction
- decreasinge2
Not reportedComparator
Verbatim
e0“Banana is sensitive to cold stress and often suffers from chilling injury”
e1“Transient overexpression of miR156c resulted in a more severe chilling phenotype”
e2“by decreasing the expression of MaSPL4 and miR528”
Claim · model-written summary
Silencing of STTM-miR156c alleviated browning in banana.
Not reportedComparator
Verbatim
e0“Banana is sensitive to cold stress and often suffers from chilling injury”
e1“the browning was alleviated in STTM-miR156c silencing and OE-MaSPL4 samples”
Claim · model-written summary
Overexpression of MaSPL4 alleviated browning in banana.
Not reportedComparator
Verbatim
e0“Banana is sensitive to cold stress and often suffers from chilling injury”
e1“the browning was alleviated in STTM-miR156c silencing and OE-MaSPL4 samples”
Claim · model-written summary
MaSPL4 may mediate transcription of genes related to lipid metabolism and antioxidation in banana (hypothesis).
Verbatim
e0“Banana is sensitive to cold stress and often suffers from chilling injury”
e1“MaSPL4 may mediate the transcription of genes related to lipid metabolism and antioxidation, in addition to the miR528-MaPPO module”
Claim · model-written summary
MaSPL4 is a master regulator of the banana fruit cold response network (author interpretation).
Verbatim
e0“Banana is sensitive to cold stress and often suffers from chilling injury”
e1“demonstrating MaSPL4 as a master regulator in the fruit cold response network”
Claim · model-written summary
The miR156c-MaSPL4 module mediates the banana chilling response by regulating the miR528-MaPPO module and other pathways (author interpretation).
Verbatim
e1“the miR156c-MaSPL4 module can mediate the chilling response in banana by regulating the miR528-MaPPO module and multiple other pathways”
The same questions in other papers
loss of function · disease · MusaPositive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.