Abstract
Abstract Long-term conservation of elite Cannabis sativa genotypes is essential to maintain genetic fidelity in clonal propagation systems. This study aimed to evaluate and optimize the D-cryoplate technique for cryopreservation of C. sativa axillary buds, with particular emphasis on the role of explant dehydration before cryostorage. Axillary buds from micropropagated plants of two cultivars were encapsulated in calcium alginate on aluminum cryoplates, osmotically pretreated, dehydrated for 0–90 minutes, and immersed in liquid nitrogen. After cryostorage, the explants were rapidly rewarmed, recovered in vitro, and subsequently acclimatized under greenhouse conditions. Shoot regeneration was strongly dependent on dehydration time, with the highest regeneration rates (63–70%) observed after 60–70 minutes of dehydration. These optimal times corresponded to capsule water content of 21–28%. Short dehydration periods resulted in low regeneration due to freezing injury, while excessive dehydration significantly reduced explant viability due to desiccation stress. Shoots regenerated from cryopreserved axillary buds showed typical morphological development with growth parameters comparable to those of the noncryopreserved control. These results demonstrate that precise control of explant water content is critical for successful cryopreservation, and the D-cryoplate technique is an efficient and chemically safe strategy for the long-term preservation of elite C. sativa clonal germplasm.
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Claims 6
Claim · model-written summary
The highest shoot regeneration rates (63-70%) were observed after 60-70 minutes of dehydration before cryostorage.
- Taxon
- Cannabis sativa
Cannabis sativat0 - Treatment
- 60–70 minutes of dehydratione1
- Outcome
- regeneration ratese1
- Direction
- higheste1
- Value
- 63–70%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e1“Shoot regeneration was strongly dependent on dehydration time, with the highest regeneration rates (63–70%) observed after 60–70 minutes of dehydration”
Claim · model-written summary
The optimal dehydration times corresponded to capsule water content of 21-28%.
- Taxon
- Cannabis sativaCannabis sativat0
- Outcome
- capsule water contente1
- Value
- 21–28%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e1“These optimal times corresponded to capsule water content of 21–28%”
Claim · model-written summary
Short dehydration periods resulted in low regeneration due to freezing injury.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- Short dehydration periodse1
- Outcome
- regeneratione1
- Direction
- lowe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e1“Short dehydration periods resulted in low regeneration due to freezing injury”
Claim · model-written summary
Excessive dehydration significantly reduced explant viability due to desiccation stress.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- excessive dehydratione1
- Outcome
- explant viabilitye1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e1“excessive dehydration significantly reduced explant viability due to desiccation stress”
Claim · model-written summary
Shoots regenerated from cryopreserved axillary buds showed typical morphological development with growth parameters comparable to the noncryopreserved control.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- cryopreserved axillary budse1
- Outcome
- growth parameterse1
- Direction
- comparablee1
- Comparator
- noncryopreserved controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e1“Shoots regenerated from cryopreserved axillary buds showed typical morphological development with growth parameters comparable to those of the noncryopreserved control”
Claim · model-written summary
The authors conclude that precise control of explant water content is critical for successful cryopreservation of C. sativa.
- Taxon
- Cannabis sativaCannabis sativat0
- Outcome
- precise control of explant water contente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa”
e1“precise control of explant water content is critical for successful cryopreservation”
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