Abstract
This study describes the standardization of an efficient in vitro propagation and hardening procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings were germinated on half-strength 1/2 MS medium supplemented with 10 g·L-1sucrose, 5.5 g·L-1agar at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·L-1TDZ, 0.1 mg·L-1NAA supported the maximal auxiliary bud multiplication rate of 3.22 per shoot tip. The proliferated buds were successfully rooted on MS medium supplemented with 0.1 mg·L-1IBA and 0.05 mg·L-1NAA resulting in 85% of the plantlets rooting. The procedure requires a 54 days cycle for the In vitro clonal propagation (14 days for shoot multiplication and 40 days for root induction) which includes 35-42 days for acclimatized plantlet production.
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Claims 4
Claim · model-written summary
MS medium with TDZ and NAA gave maximal auxiliary bud multiplication of 3.22 per shoot tip.
- Taxon
- Hemp
Cannabis sativa subsp. sativat0 - Organ / stage
- shoot tipe0
- Treatment
- MS medium containing 0.2 mg·L-1TDZ, 0.1 mg·L-1NAAe0
- Outcome
- auxiliary bud multiplication ratee0
- Direction
- maximale0
- Value
- 3.22 per shoot tipe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium containing 0.2 mg·L-1TDZ, 0.1 mg·L-1NAA supported the maximal auxiliary bud multiplication rate of 3.22 per shoot tip.”
t0“Hemp”
Claim · model-written summary
Rooting on MS medium with IBA and NAA reached 85% of plantlets.
- Taxon
- HempCannabis sativa subsp. sativat0
- Organ / stage
- proliferated budse0
- Treatment
- MS medium supplemented with 0.1 mg·L-1IBA and 0.05 mg·L-1NAAe0
- Outcome
- plantlets rootinge0
- Direction
- successfully rootede0
- Value
- 85%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The proliferated buds were successfully rooted on MS medium supplemented with 0.1 mg·L-1IBA and 0.05 mg·L-1NAA resulting in 85% of the plantlets rooting.”
t0“Hemp”
Claim · model-written summary
The clonal propagation procedure requires a 54 days cycle, including 14 days for shoot multiplication and 40 days for root induction.
- Taxon
- HempCannabis sativa subsp. sativat0
- Outcome
- In vitro clonal propagatione0
- Value
- 54 dayse0
- Qualifier
- 14 days for shoot multiplication and 40 days for root inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The procedure requires a 54 days cycle for the In vitro clonal propagation (14 days for shoot multiplication and 40 days for root induction) which includes 35-42 days for acclimatized plantlet production.”
t0“Hemp”
Claim · model-written summary
Acclimatized plantlet production takes 35-42 days in the procedure.
- Taxon
- HempCannabis sativa subsp. sativat0
- Outcome
- acclimatized plantlet productione0
- Value
- 35-42 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which includes 35-42 days for acclimatized plantlet production.”
t0“Hemp”
The same questions in other papers
in vitro · growth · Cannabis sativaPositive and negative results
10 papers in the same direction as this paper · 3 in the opposite direction
- positive
- no effect
- negative
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See all 14 papers →in vitro · pest · Cannabis sativaResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.