Paper · 2026 · Journal of soil science and plant nutrition

Biochar-Modified Sulfur and Licorice Root Extract Improve Rhizosphere Properties and Biochemical Adaptation in Cucurbita pepo Plants Under Salt Stress

Nasr M. Abdou; Mohammed A. H. Gyushi; Basma R. Abdel-Moatamed; et al.

9
claims
15
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

Abstract Soil salinity critically threatens agricultural sustainability, particularly in squash production. This study evaluates integrated soil-plant strategies to mitigate salt stress, applying biochar-modified sulfur (BMS) as a soil amendment and licorice root extract (LRE) as a foliar treatment to enhance soil functionality and plant osmotic resilience. Two field experiments (2023–2024) employed a split-plot in randomized complete block design. Main plots tested BMS at 0 (BMS₀), 5 (BMS₅), and 10 (BMS₁₀) ton ha⁻¹; subplots received foliar LRE at 0% (LRE₀) or 1% (LRE₁). BMS₅ and BMS₁₀ significantly ameliorated key physicochemical constraints of saline soils: reducing bulk density, saturated hydraulic conductivity, electrical conductivity (ECe), and pH, while increasing soil-water retention capacity, soil porosity, available water content, organic matter content, cation exchange capacity and nutrient availability (nitrogen, phosphorus and potassium) versus BMS₀. Synergistic BMS + LRE₁ treatments enhanced squash growth metrics, photosynthetic pigments, nutritional and water status, membrane integrity, and yield over (BMS₀ + LRE₀). However, leaf proline, phenolics, free amino acids and TSS were significantly reduced, under the same treatments, confirming improved salinity tolerance of squash plants. Combined BMS and LRE application sustainably enhances saline soil properties and fortifies plant biochemical resilience, optimizing squash productivity. This strategy offers a viable pathway for saline soil management in water-scarce agroecosystems.

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Claims 9

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

BMS treatments reduced saline-soil bulk density, saturated hydraulic conductivity, and electrical conductivity versus BMS₀.

Taxon
squashe1
Treatment
BMS₅ and BMS₁₀e0
Outcome
bulk density, saturated hydraulic conductivity, electrical conductivity (ECe)e0
Direction
reducinge0
Comparator
BMS₀e0

Verbatim

e0

“BMS₅ and BMS₁₀ significantly ameliorated key physicochemical constraints of saline soils: reducing bulk density, saturated hydraulic conductivity, electrical conductivity (ECe), and pH, while increasing soil-water retention capacity, soil porosity, available water content, organic matter content, cation exchange capacity and nutrient availability (nitrogen, phosphorus and potassium) versus BMS₀”

✓ located verbatim in the source · abstract, characters 579–976 · show in text ↑
e1

“squash production”

✓ located verbatim in the source · abstract, characters 89–106 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

BMS treatments reduced saline-soil pH versus BMS₀.

Taxon
squashe1
Treatment
BMS₅ and BMS₁₀e0
Outcome
pHe0
Direction
reducinge0
Comparator
BMS₀e0

Verbatim

e0

“BMS₅ and BMS₁₀ significantly ameliorated key physicochemical constraints of saline soils: reducing bulk density, saturated hydraulic conductivity, electrical conductivity (ECe), and pH, while increasing soil-water retention capacity, soil porosity, available water content, organic matter content, cation exchange capacity and nutrient availability (nitrogen, phosphorus and potassium) versus BMS₀”

✓ located verbatim in the source · abstract, characters 579–976 · show in text ↑
e1

“squash production”

✓ located verbatim in the source · abstract, characters 89–106 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

BMS treatments increased saline-soil water retention, porosity, and available water versus BMS₀.

Taxon
squashe1
Treatment
BMS₅ and BMS₁₀e0
Outcome
soil-water retention capacity, soil porosity, available water contente0
Direction
increasinge0
Comparator
BMS₀e0

Verbatim

e0

“BMS₅ and BMS₁₀ significantly ameliorated key physicochemical constraints of saline soils: reducing bulk density, saturated hydraulic conductivity, electrical conductivity (ECe), and pH, while increasing soil-water retention capacity, soil porosity, available water content, organic matter content, cation exchange capacity and nutrient availability (nitrogen, phosphorus and potassium) versus BMS₀”

✓ located verbatim in the source · abstract, characters 579–976 · show in text ↑
e1

“squash production”

✓ located verbatim in the source · abstract, characters 89–106 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

BMS treatments increased saline-soil organic matter, cation exchange capacity, and nutrient availability versus BMS₀.

Taxon
squashe1
Treatment
BMS₅ and BMS₁₀e0
Outcome
organic matter content, cation exchange capacity and nutrient availability (nitrogen, phosphorus and potassium)e0
Direction
increasinge0
Comparator
BMS₀e0

Verbatim

e0

“BMS₅ and BMS₁₀ significantly ameliorated key physicochemical constraints of saline soils: reducing bulk density, saturated hydraulic conductivity, electrical conductivity (ECe), and pH, while increasing soil-water retention capacity, soil porosity, available water content, organic matter content, cation exchange capacity and nutrient availability (nitrogen, phosphorus and potassium) versus BMS₀”

✓ located verbatim in the source · abstract, characters 579–976 · show in text ↑
e1

“squash production”

✓ located verbatim in the source · abstract, characters 89–106 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined BMS and LRE₁ enhanced squash growth metrics, pigments, and nutritional status versus untreated plots.

Taxon
squashe0
Treatment
BMS + LRE₁e0
Outcome
growth metrics, photosynthetic pigments, nutritional and water statuse0
Direction
enhancede0
Comparator
BMS₀ + LRE₀e0

Verbatim

e0

“Synergistic BMS + LRE₁ treatments enhanced squash growth metrics, photosynthetic pigments, nutritional and water status, membrane integrity, and yield over (BMS₀ + LRE₀)”

✓ located verbatim in the source · abstract, characters 978–1147 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined BMS and LRE₁ enhanced squash membrane integrity and yield versus untreated plots.

Taxon
squashe0
Treatment
BMS + LRE₁e0
Outcome
membrane integrity, and yielde0
Direction
enhancede0
Comparator
BMS₀ + LRE₀e0

Verbatim

e0

“Synergistic BMS + LRE₁ treatments enhanced squash growth metrics, photosynthetic pigments, nutritional and water status, membrane integrity, and yield over (BMS₀ + LRE₀)”

✓ located verbatim in the source · abstract, characters 978–1147 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined treatments significantly reduced squash leaf proline, phenolics, and free amino acids.

Taxon
squashe1
Treatment
BMS + LRE₁e1
Outcome
leaf proline, phenolics, free amino acidse0
Direction
reducede0

Not reportedComparator

Verbatim

e0

“leaf proline, phenolics, free amino acids and TSS were significantly reduced, under the same treatments”

✓ located verbatim in the source · abstract, characters 1158–1261 · show in text ↑
e1

“Synergistic BMS + LRE₁ treatments enhanced squash growth metrics”

✓ located verbatim in the source · abstract, characters 978–1042 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined treatments significantly reduced squash leaf TSS.

Taxon
squashe1
Treatment
BMS + LRE₁e1
Outcome
TSSe0
Direction
reducede0

Not reportedComparator

Verbatim

e0

“leaf proline, phenolics, free amino acids and TSS were significantly reduced, under the same treatments”

✓ located verbatim in the source · abstract, characters 1158–1261 · show in text ↑
e1

“Synergistic BMS + LRE₁ treatments enhanced squash growth metrics”

✓ located verbatim in the source · abstract, characters 978–1042 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors interpret combined BMS and LRE as improving squash salinity tolerance and productivity.

Taxon
squashe0
Outcome
BMS and LRE applicatione0

Verbatim

e0

“Combined BMS and LRE application sustainably enhances saline soil properties and fortifies plant biochemical resilience, optimizing squash productivity”

✓ located verbatim in the source · abstract, characters 1320–1471 · show in text ↑

biochar · physiology: osmolytes · Cucurbita (pumpkins)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

biochar · physiology: photosynthesis · Cucurbita (pumpkins)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
    Those three media improved leaf pigment and N, P and K contents.

    Alternative Growing Media for Producing Squash Transplants in North Sinai Region · 2023 · compared with commercial media + soil and commercial media + chicken manure

    Same direction

See all 2 papers →

biochar · yield · Cucurbita (pumpkins)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.