Paper · 2025 · Archivo Digital UPM (Universidad Politécnica de Madrid)

Growth and Photosynthetic Responses of Lactuca sativa L. to Different Zinc Fertilizer Sources and Applications

Francisco Hernández, Marina de; Hernández Montes, Esther; DeSanto, Sarah; et al.

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17
verbatim quotes
0
citations
2
research areas
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Open access · cc-by-nc-nd

Abstract

Zinc (Zn) is an essential micronutrient for plant growth, serving as a co-factor in enzymatic processes and pigment biosynthesis. In horticultural crops such as lettuce, Zn fertilization is increasingly relevant for optimizing yield and nutritional quality. In this study, a greenhouse pot experiment was conducted using Lactuca sativa L. cv. Romana Verano (Ramiro Arnedo) to evaluate the effects of four Zn sources with contrasting physio-chemical properties-ZnSO4, a synthetic chelate containing DTPA, EDTA, and HEDTA, a Zn-lignosulphonate complex, and ZnO nanoparticles-applied to soil at rates of 15, 30, 60, and 120 mg Znkg-1. Morphometric traits, photosynthetic pigmentation, and photosystem performance were assessed to determine differences in plant response. Results showed that low to moderate Zn supply (15-60 mg Znkg-1) maintained growth, leaf number, stem diameter, and biomass without significant changes compared to the control. In contrast, the highest dose (120 mg Znkg-1), particularly in chelated forms, led to reductions in growth and yield exceeding 80%, reflecting supra-optimal effects. Although lignosulphonate and nanoparticles sources lowered soil Zn availability, they did not affect lettuce growth or yield, indicating their potential as safer agricultural alternatives to conventional Zn fertilizers. Photosynthetic efficiency, measured through chlorophyll fluorescence and electron transport activity, was positively modulated by adequate Zn levels but declined at excessive concentrations. These findings highlight that Zn efficiency strongly depends on its chemical form and applied dose, providing practical insights for optimizing Zn fertilization strategies in lettuce and other horticultural crops.

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Claims 8

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Low to moderate Zn supply (15-60 mg Znkg-1) does not change growth, leaf number or stem diameter of lettuce compared to the control.

Taxon
Lactuca sativa L.Lactuca sativat0
Treatment
low to moderate Zn supply (15-60 mg Znkg-1)e1
Outcome
growth, leaf number, stem diametere1
Direction
without significant changese1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lactuca sativa L.”

✓ located verbatim in the source · title, characters 39–56 · show in text ↑
e1

“Results showed that low to moderate Zn supply (15-60 mg Znkg-1) maintained growth, leaf number, stem diameter, and biomass without significant changes compared to the control”

✓ located verbatim in the source · abstract, characters 768–942 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Low to moderate Zn supply (15-60 mg Znkg-1) does not change lettuce biomass compared to the control.

Taxon
Lactuca sativa L.Lactuca sativat0
Treatment
low to moderate Zn supply (15-60 mg Znkg-1)e1
Outcome
biomasse1
Direction
without significant changese1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lactuca sativa L.”

✓ located verbatim in the source · title, characters 39–56 · show in text ↑
e1

“Results showed that low to moderate Zn supply (15-60 mg Znkg-1) maintained growth, leaf number, stem diameter, and biomass without significant changes compared to the control”

✓ located verbatim in the source · abstract, characters 768–942 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The highest Zn dose (120 mg Znkg-1), particularly in chelated forms, reduces lettuce growth and yield by more than 80% relative to the control.

Taxon
Lactuca sativa L.Lactuca sativat0
Treatment
highest dose (120 mg Znkg-1), particularly in chelated formse2
Outcome
growth and yielde2
Direction
reductionse2
Value
exceeding 80%e2
Qualifier
reflecting supra-optimal effectse2
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lactuca sativa L.”

✓ located verbatim in the source · title, characters 39–56 · show in text ↑
e1

“Results showed that low to moderate Zn supply (15-60 mg Znkg-1) maintained growth, leaf number, stem diameter, and biomass without significant changes compared to the control”

✓ located verbatim in the source · abstract, characters 768–942 · show in text ↑
e2

“In contrast, the highest dose (120 mg Znkg-1), particularly in chelated forms, led to reductions in growth and yield exceeding 80%, reflecting supra-optimal effects”

✓ located verbatim in the source · abstract, characters 944–1108 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Lignosulphonate and nanoparticles Zn sources lower soil Zn availability.

Taxon
Lactuca sativa L.Lactuca sativat0
Treatment
lignosulphonate and nanoparticles sourcese3
Outcome
soil Zn availabilitye3
Direction
lowerede3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lactuca sativa L.”

✓ located verbatim in the source · title, characters 39–56 · show in text ↑
e3

“Although lignosulphonate and nanoparticles sources lowered soil Zn availability, they did not affect lettuce growth or yield, indicating their potential as safer agricultural alternatives to conventional Zn fertilizers”

✓ located verbatim in the source · abstract, characters 1110–1328 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Lignosulphonate and nanoparticles Zn sources do not affect lettuce growth or yield.

Taxon
Lactuca sativa L.Lactuca sativat0
Treatment
lignosulphonate and nanoparticles sourcese3
Outcome
growth or yielde3
Direction
did not affecte3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lactuca sativa L.”

✓ located verbatim in the source · title, characters 39–56 · show in text ↑
e3

“Although lignosulphonate and nanoparticles sources lowered soil Zn availability, they did not affect lettuce growth or yield, indicating their potential as safer agricultural alternatives to conventional Zn fertilizers”

✓ located verbatim in the source · abstract, characters 1110–1328 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Lignosulphonate and nanoparticles Zn sources are indicated as potentially safer agricultural alternatives to conventional Zn fertilizers.

Taxon
Lactuca sativa L.Lactuca sativat0
Outcome
potential as safer agricultural alternativese3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lactuca sativa L.”

✓ located verbatim in the source · title, characters 39–56 · show in text ↑
e3

“Although lignosulphonate and nanoparticles sources lowered soil Zn availability, they did not affect lettuce growth or yield, indicating their potential as safer agricultural alternatives to conventional Zn fertilizers”

✓ located verbatim in the source · abstract, characters 1110–1328 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adequate Zn levels positively modulate photosynthetic efficiency of lettuce.

Taxon
Lactuca sativa L.Lactuca sativat0
Treatment
adequate Zn levelse4
Outcome
Photosynthetic efficiencye4
Direction
positively modulatede4
Qualifier
measured through chlorophyll fluorescence and electron transport activitye4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lactuca sativa L.”

✓ located verbatim in the source · title, characters 39–56 · show in text ↑
e4

“Photosynthetic efficiency, measured through chlorophyll fluorescence and electron transport activity, was positively modulated by adequate Zn levels but declined at excessive concentrations”

✓ located verbatim in the source · abstract, characters 1330–1519 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Excessive Zn concentrations decrease photosynthetic efficiency of lettuce.

Taxon
Lactuca sativa L.Lactuca sativat0
Treatment
excessive concentrationse4
Outcome
Photosynthetic efficiencye4
Direction
declinede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

t0

“Lactuca sativa L.”

✓ located verbatim in the source · title, characters 39–56 · show in text ↑
e4

“Photosynthetic efficiency, measured through chlorophyll fluorescence and electron transport activity, was positively modulated by adequate Zn levels but declined at excessive concentrations”

✓ located verbatim in the source · abstract, characters 1330–1519 · show in text ↑

zinc atom · growth · Lactuca sativa (garden lettuce)Results differ

15 papers: 7 positive, 8 negative or no effect

7 papers in the same direction as this paper · 7 in the opposite direction

  1. positive
  2. positive
  3. positive

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zinc atom · physiology: photosynthesis · Lactuca sativa (garden lettuce)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

2 in the opposite direction

  1. no effect
  2. negative
See all 3 papers →

nanoparticle · yield · Lactuca sativa (garden lettuce)Positive and negative results

5 papers: 4 positive, 1 negative or no effect

4 in the opposite direction

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.