Abstract
Polymethoxylated flavones (PMFs) are a class of flavonoid compounds predominantly present in citrus and are renowned for their extensive biological activity (antiobesity, antioxidant and cardiovascular protection). Recent advancements have elucidated the biosynthetic pathway of PMFs, although the transcriptional regulatory mechanisms involved are not yet fully understood. In this study, we used PMF-rich Bingtangcheng ( Citrus sinensis Osbeck) fruit (BTC) and treated it with exogenous methyl salicylate (MeSA). Our study demonstrates that MeSA treatment dynamically regulates the biosynthesis of PMF monomers in citrus peels during low-temperature storage (10 °C), with 0.2 mmol · L -1 MeSA combined with 14-day storage effectively enhancing PMF accumulation. The transcriptomic analyses demonstrated that MeSA treatment activated the expression of essential genes associated with the PMF biosynthetic pathway, including FNSII , F3'H , and COMT . Additionally, MeSA also activated the biosynthesis and signaling pathways of other phytohormones. A transcription factor, CitERF92 , from the ERF family B3 group, was significantly induced by MeSA and strongly correlated with the expression of CitFNSII-1 . Dual-luciferase assays and electrophoretic mobility shift assays demonstrated that CitERF92 could bind to and significantly activate the promoter activity of CitFNSII-1 . Subsequent transient overexpression experiments confirmed that CitERF92 markedly increased the content of PMF monomers in the BTC peel. These results reveal the CitERF92-CitFNSII-1 module as a novel regulator of PMF biosynthesis, enhancing the understanding of the regulatory network that controls PMF production in citrus.
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Claims 7
Claim · model-written summary
MeSA at 0.2 mmol per liter for 14 days enhanced PMF accumulation.
- Treatment
- 0.2 mmol · L -1 MeSA combined with 14-day storagee0
- Outcome
- PMF accumulatione0
- Direction
- enhancinge0
CitrusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.2 mmol · L -1 MeSA combined with 14-day storage effectively enhancing PMF accumulation.”
t0“citrus”
Claim · model-written summary
MeSA activated PMF biosynthetic genes FNSII, F3H and COMT.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeSA treatment activated the expression of essential genes associated with the PMF biosynthetic pathway, including FNSII , F3'H , and COMT .”
t0“citrus”
Claim · model-written summary
MeSA activated other phytohormone biosynthesis and signaling.
- Treatment
- MeSAe0
- Outcome
- biosynthesis and signaling pathways of other phytohormonese0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeSA also activated the biosynthesis and signaling pathways of other phytohormones.”
t0“citrus”
Claim · model-written summary
MeSA significantly induced CitERF92.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CitERF92 , from the ERF family B3 group, was significantly induced by MeSA and strongly correlated with the expression of CitFNSII-1 .”
t0“citrus”
Claim · model-written summary
CitERF92 correlated with CitFNSII-1 expression.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CitERF92 , from the ERF family B3 group, was significantly induced by MeSA and strongly correlated with the expression of CitFNSII-1 .”
t0“citrus”
Claim · model-written summary
CitERF92 bound and activated CitFNSII-1 promoter.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CitERF92 could bind to and significantly activate the promoter activity of CitFNSII-1 .”
t0“citrus”
Claim · model-written summary
CitERF92 overexpression increased peel PMF monomers.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CitERF92 markedly increased the content of PMF monomers in the BTC peel.”
t0“citrus”
The same questions in other papers
storage · molecular: metabolites · CitrusPositive and negative results
2 in the opposite direction
- no effect
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.