Abstract
Elongins A (ELOA), B (ELOB), and C (ELOC) were originally identified in mammals as transcription elongation complex. Beyond this role, the three mammalian elongins function in E3 ligase complex that degrades the large subunit of the stalled RNA polymerase II after DNA damage. S. cerevisiae lacks ELOB but posseses ELOA and ELOC homologs which form a similar E3 ligase complex that performs a comparable function in targeting stalled RNA polymerase II for degradation. These yeast homologs are functionally conserved with their mammalian counterparts, as yeast ELOC enhances mammalian ELOA transcript elongation via interacting with it. However, yeast ELOA/C complex alone does not promote yeast transcriptional elongation. Their role in RNA polymerase II polyubiquitylation and degradation following DNA damage has been well characterized. In this study, we identified Arabidopsis elongin A homologue (ELOA; At2g42780) and examined the roles of ELOA and elongin C homologue (ELOC; At5g59140) in UV tolerance and development using molecular and genetic analyses. YFP-ELOA localized to nucleus and this pattern was not affected after UV treatment. However, YFP-ELOC localized to both nuclei and cytosol with reduced cytoplasmic signal post-UV exposure. Both Ateloa-2 and Ateloc-3 mutants exhibited enhanced UV-sensitive phenotypes in seedlings and adults. ELOA overexpression enhanced hypocotyl UV tolerance. Yeast two-hybrid assay confirmed the interaction between Arabidopsis ELOA and ELOC. Interestingly, ELOA and ELOC overexpression increased silique length and seed number, and ELOC overexpression also increased plant height. These findings demonstrate that Arabidopsis ELOA and ELOC homologs are required for UV tolerance and contribute to plant developmental regulation.
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Claims 7
Claim · model-written summary
YFP-ELOA stayed nuclear and its localization was not affected by UV treatment in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- UV treatmente1
- Gene
- YFP-ELOAe1
- Outcome
- YFP-ELOA localized to nucleuse1
- Direction
- not affectede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified Arabidopsis elongin A homologue (ELOA; At2g42780) and examined the roles of ELOA and elongin C homologue (ELOC; At5g59140) in UV tolerance and development”
e1“YFP-ELOA localized to nucleus and this pattern was not affected after UV treatment”
Claim · model-written summary
YFP-ELOC showed reduced cytoplasmic signal after UV exposure in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- post-UV exposuree2
- Gene
- YFP-ELOCe2
- Outcome
- cytoplasmic signale2
- Direction
- reducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified Arabidopsis elongin A homologue (ELOA; At2g42780) and examined the roles of ELOA and elongin C homologue (ELOC; At5g59140) in UV tolerance and development”
e2“YFP-ELOC localized to both nuclei and cytosol with reduced cytoplasmic signal post-UV exposure”
Claim · model-written summary
Ateloa-2 and Ateloc-3 mutants showed enhanced UV-sensitive phenotypes in seedlings and adults.
- Taxon
- ArabidopsisArabidopsise0
- Material
- Ateloa-2 and Ateloc-3 mutantse3
- Organ / stage
- seedlings and adultse3
- Treatment
- UVe3
- Outcome
- UV-sensitive phenotypese3
- Direction
- enhancede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified Arabidopsis elongin A homologue (ELOA; At2g42780) and examined the roles of ELOA and elongin C homologue (ELOC; At5g59140) in UV tolerance and development”
e3“Both Ateloa-2 and Ateloc-3 mutants exhibited enhanced UV-sensitive phenotypes in seedlings and adults”
Claim · model-written summary
ELOA overexpression enhanced hypocotyl UV tolerance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- hypocotyle4
- Treatment
- ELOA overexpressione4
- Gene
- ELOAe4
- Outcome
- hypocotyl UV tolerancee4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified Arabidopsis elongin A homologue (ELOA; At2g42780) and examined the roles of ELOA and elongin C homologue (ELOC; At5g59140) in UV tolerance and development”
e4“ELOA overexpression enhanced hypocotyl UV tolerance”
Claim · model-written summary
A yeast two-hybrid assay confirmed the interaction between Arabidopsis ELOA and ELOC.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- ELOA and ELOCe5
- Outcome
- interaction between Arabidopsis ELOA and ELOCe5
- Qualifier
- Yeast two-hybrid assaye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified Arabidopsis elongin A homologue (ELOA; At2g42780) and examined the roles of ELOA and elongin C homologue (ELOC; At5g59140) in UV tolerance and development”
e5“Yeast two-hybrid assay confirmed the interaction between Arabidopsis ELOA and ELOC”
Claim · model-written summary
ELOA and ELOC overexpression increased silique length and seed number in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- ELOA and ELOC overexpressione6
- Gene
- ELOA and ELOCe6
- Outcome
- silique length and seed numbere6
- Direction
- increasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified Arabidopsis elongin A homologue (ELOA; At2g42780) and examined the roles of ELOA and elongin C homologue (ELOC; At5g59140) in UV tolerance and development”
e6“ELOA and ELOC overexpression increased silique length and seed number”
Claim · model-written summary
ELOC overexpression increased plant height in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- ELOC overexpressione7
- Gene
- ELOCe7
- Outcome
- plant heighte7
- Direction
- increasede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified Arabidopsis elongin A homologue (ELOA; At2g42780) and examined the roles of ELOA and elongin C homologue (ELOC; At5g59140) in UV tolerance and development”
e7“ELOC overexpression also increased plant height”
The same questions in other papers
uv · molecular: metabolites · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
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