Abstract
Arabidopsis thaliana UV RESISTANCE LOCUS8 (UVR8) is a UV-B-specific signaling component that binds to chromatin and regulates UV protection by orchestrating expression of a range of genes. Here, we studied how UV-B regulates UVR8. We show that UV-B stimulates the nuclear accumulation of both a green fluorescent protein (GFP)-UVR8 fusion and native UVR8. Nuclear accumulation leads to UV-B induction of the HY5 gene, encoding a key transcriptional effector of the UVR8 pathway. Nuclear accumulation of UVR8 is specific to UV-B, occurs at low fluence rates, and is observed within 5 min of UV-B exposure. Attachment of a nuclear export signal (NES) to GFP-UVR8 causes cytosolic localization in the absence of UV-B. However, UV-B promotes rapid nuclear accumulation of NES-GFP-UVR8, indicating a concerted mechanism for nuclear translocation. UVR8 lacking the N-terminal 23 amino acids is impaired in nuclear translocation. Attachment of a nuclear localization signal (NLS) to UVR8 causes constitutive nuclear localization. However, NLS-GFP-UVR8 only confers HY5 gene expression following UV-B illumination, indicating that nuclear localization, although necessary for UVR8 function, is insufficient to cause expression of target genes; UV-B is additionally required to stimulate UVR8 function in the nucleus. These findings provide new insights into the mechanisms through which UV-B regulates gene expression in plants.
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Claims 11
Claim · model-written summary
UV-B stimulated nuclear accumulation of GFP-UVR8 and native UVR8.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- UV-Be0
- Gene
- UVR8e0
- Outcome
- nuclear accumulatione0
- Direction
- stimulatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We show that UV-B stimulates the nuclear accumulation of both a green fluorescent protein (GFP)-UVR8 fusion and native UVR8.”
t0“Arabidopsis”
Claim · model-written summary
Nuclear UVR8 accumulation led to UV-B induction of HY5.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- HY5e0
- Outcome
- UV-B induction of the HY5 genee0
- Qualifier
- Nuclear accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nuclear accumulation leads to UV-B induction of the HY5 gene, encoding a key transcriptional effector of the UVR8 pathway.”
t0“Arabidopsis”
Claim · model-written summary
UVR8 nuclear accumulation was specific to UV-B.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- UVR8e0
- Outcome
- Nuclear accumulation of UVR8e0
- Qualifier
- specific to UV-Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nuclear accumulation of UVR8 is specific to UV-B, occurs at low fluence rates, and is observed within 5 min of UV-B exposure.”
t0“Arabidopsis”
Claim · model-written summary
UVR8 nuclear accumulation occurred at low UV-B fluence rates.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- Nuclear accumulation of UVR8e0
- Qualifier
- low fluence ratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nuclear accumulation of UVR8 is specific to UV-B, occurs at low fluence rates, and is observed within 5 min of UV-B exposure.”
t0“Arabidopsis”
Claim · model-written summary
UVR8 nuclear accumulation was observed within 5 min of UV-B exposure.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- Nuclear accumulation of UVR8e0
- Value
- 5 mine0
- Qualifier
- UV-B exposuree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nuclear accumulation of UVR8 is specific to UV-B, occurs at low fluence rates, and is observed within 5 min of UV-B exposure.”
t0“Arabidopsis”
Claim · model-written summary
An NES attached to GFP-UVR8 caused cytosolic localization without UV-B.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Attachment of a nuclear export signal (NES) to GFP-UVR8e0
- Gene
- GFP-UVR8e0
- Outcome
- cytosolic localizatione0
- Qualifier
- absence of UV-Be0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Attachment of a nuclear export signal (NES) to GFP-UVR8 causes cytosolic localization in the absence of UV-B.”
t0“Arabidopsis”
Claim · model-written summary
UV-B promoted rapid nuclear accumulation of NES-GFP-UVR8.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- UV-Be0
- Gene
- NES-GFP-UVR8e0
- Outcome
- nuclear accumulation of NES-GFP-UVR8e0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, UV-B promotes rapid nuclear accumulation of NES-GFP-UVR8, indicating a concerted mechanism for nuclear translocation.”
t0“Arabidopsis”
Claim · model-written summary
UVR8 lacking its N-terminal 23 amino acids showed impaired nuclear translocation.
- Taxon
- ArabidopsisArabidopsist0
- Material
- UVR8 lacking the N-terminal 23 amino acidse0
- Gene
- UVR8e0
- Outcome
- nuclear translocatione0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UVR8 lacking the N-terminal 23 amino acids is impaired in nuclear translocation.”
t0“Arabidopsis”
Claim · model-written summary
An NLS attached to UVR8 caused constitutive nuclear localization.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Attachment of a nuclear localization signal (NLS) to UVR8e0
- Gene
- UVR8e0
- Outcome
- constitutive nuclear localizatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Attachment of a nuclear localization signal (NLS) to UVR8 causes constitutive nuclear localization.”
t0“Arabidopsis”
Claim · model-written summary
NLS-GFP-UVR8 conferred HY5 expression only after UV-B illumination.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- UV-B illuminatione0
- Gene
- HY5e0
- Outcome
- HY5 gene expressione0
- Qualifier
- onlye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, NLS-GFP-UVR8 only confers HY5 gene expression following UV-B illumination, indicating that nuclear localization, although necessary for UVR8 function, is insufficient to cause expression of target genes; UV-B is additionally required to stimulate UVR8 function in the nucleus.”
t0“Arabidopsis”
Claim · model-written summary
Nuclear localization was necessary but insufficient for UVR8 target gene expression, which additionally required UV-B.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- UVR8e0
- Outcome
- expression of target genese0
- Qualifier
- UV-B is additionally requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nuclear localization, although necessary for UVR8 function, is insufficient to cause expression of target genes; UV-B is additionally required to stimulate UVR8 function in the nucleus.”
t0“Arabidopsis”
The same questions in other papers
uv · molecular: metabolites · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- no effect
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.