Abstract
Plant phenotype stability during ex vitro growth, one of the main requirements of plant micropropagation, was tested on tobacco. Plants cultivated in vitro in the presence of 3 % sucrose under photon flux density (PFD) of 200 μmol m-2 s-1 (3 % HL plants) showed the best growth and photosynthetic parameters in the course of 7-day acclimation. However, significant change in phenotype of these plants appeared under a decrease in PFD to 50 μmol m-2 s-1 during further ex vitro growth (in the period of 7th - 17th day). Much higher internodia elongation was found in 3 % HL plants in comparison with plants grown in vitro on sucrose media under PFD of 50 μmol m-2 s-1 (3 % LL) or without sucrose either under PFD of 50 μmol m-2 s-1 or 200 μmol m-2 s-1 (0 % LL, 0 % HL). It can be presumed that 3 % HL plants show permanent demand for high PFD. Neither ABA or chlorophyll contents nor de novo thylakoid membrane synthesis were related to the morphogenic effect of low PFD. Changeable contents of hexoses in leaves of 3 % HL and 3 % LL plants were in no direct correlation to the elongated growth.
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Claims 6
Claim · model-written summary
Tobacco plants cultivated in vitro with 3 % sucrose under 200 umol m-2 s-1 PFD (3 % HL) showed the best growth and photosynthetic parameters during 7-day ex vitro acclimation.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- 3 % sucrose under photon flux density (PFD) of 200 μmol m-2 s-1e1
- Outcome
- growth and photosynthetic parameterse1
- Direction
- beste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Plants cultivated in vitro in the presence of 3 % sucrose under photon flux density (PFD) of 200 μmol m-2 s-1 (3 % HL plants) showed the best growth and photosynthetic parameters”
e0“tobacco”
Claim · model-written summary
A significant change in phenotype of 3 % HL plants appeared when PFD was decreased to 50 umol m-2 s-1 during further ex vitro growth (days 7 to 17).
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- decrease in PFD to 50 μmol m-2 s-1e1
- Outcome
- change in phenotypee1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“significant change in phenotype of these plants appeared under a decrease in PFD to 50 μmol m-2 s-1 during further ex vitro growth”
e0“tobacco”
Claim · model-written summary
Internode elongation was much higher in 3 % HL plants than in 3 % LL plants or plants grown without sucrose under either PFD.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- 3 % HL plantse1
- Outcome
- internodia elongatione1
- Direction
- Much highere1
- Comparator
- plants grown in vitro on sucrose media under PFD of 50 μmol m-2 s-1 (3 % LL) or without sucrose either under PFD of 50 μmol m-2 s-1 or 200 μmol m-2 s-1 (0 % LL, 0 % HL)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Much higher internodia elongation was found in 3 % HL plants in comparison with plants grown in vitro on sucrose media under PFD of 50 μmol m-2 s-1 (3 % LL) or without sucrose either under PFD of 50 μmol m-2 s-1 or 200 μmol m-2 s-1 (0 % LL, 0 % HL)”
e0“tobacco”
Claim · model-written summary
Neither ABA content, chlorophyll content nor de novo thylakoid membrane synthesis was related to the morphogenic effect of low PFD.
- Taxon
- tobaccoNicotiana tabacume0
- Compound
- ABAe1
- Outcome
- morphogenic effect of low PFDe1
- Direction
- no_changeinferrede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Neither ABA or chlorophyll contents nor de novo thylakoid membrane synthesis were related to the morphogenic effect of low PFD”
e0“tobacco”
Claim · model-written summary
Changeable hexose contents in leaves of 3 % HL and 3 % LL plants were in no direct correlation to the elongated growth.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- leavese1
- Compound
- hexosese1
- Outcome
- elongated growthe1
- Direction
- no_changeinferrede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Changeable contents of hexoses in leaves of 3 % HL and 3 % LL plants were in no direct correlation to the elongated growth”
e0“tobacco”
Claim · model-written summary
The authors presume that 3 % HL plants show a permanent demand for high PFD.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- 3 % HL plantse1
- Outcome
- permanent demand for high PFDe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“It can be presumed that 3 % HL plants show permanent demand for high PFD”
e0“tobacco”
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See all 29 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.