Abstract
An efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. As cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency callus culture. Different carbon sources such as fructose, glucose, sucrose and maltose were tested at various concentrations to control phenolic secretion in callus culture. Among them, 3% maltose was found to be the best carbon source for effectively controlling phenolic secretion in callus induction medium. High frequency of callus induction was obtained on MSB5 medium supplemented with 3% Maltose, 2,4-D (0.90 μM) and Kinetin (4.60 μM) from both cotyledon and hypocotyl explants. The best result of callus induction was obtained with hypocotyl explant (94.90%) followed by cotyledon explant (85.20%). MSB5 medium supplemented with 2,4-D (0.45 μM) along with 2iP (2.95 μM) gave tremendous proliferation of callus with high percentage of response. Varying degrees of colors and textures of callus were observed under different hormone treatments. The present study offers a solution for controlling phenolic secretion in cotton callus culture by adjusting carbon sources without adding any additives and evaluates the manipulation of plant growth regulators for efficient callus culture of SVPR-2 cotton cultivar.
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Claims 6
Claim · model-written summary
Three percent maltose best controlled phenolic secretion in cotton callus induction medium.
- Taxon
- cotton
Gossypium hirsutumt0 - Material
- SVPR-2t0
- Treatment
- 3% maltosee0
- Outcome
- phenolic secretione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“3% maltose was found to be the best carbon source for effectively controlling phenolic secretion in callus induction medium”
t0“cotton (Gossypium hirsutum L.) cv. SVPR-2”
Claim · model-written summary
The supplemented MSB5 medium gave high callus induction from cotyledon and hypocotyl explants.
- Taxon
- cottonGossypium hirsutumt0
- Material
- SVPR-2t0
- Organ / stage
- cotyledon and hypocotyl explantse0
- Treatment
- MSB5 medium supplemented with 3% Maltose, 2,4-D (0.90 μM) and Kinetin (4.60 μM)e0
- Outcome
- callus inductione0
- Direction
- High frequencye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High frequency of callus induction was obtained on MSB5 medium supplemented with 3% Maltose, 2,4-D (0.90 μM) and Kinetin (4.60 μM) from both cotyledon and hypocotyl explants”
t0“cotton (Gossypium hirsutum L.) cv. SVPR-2”
Claim · model-written summary
Hypocotyl explants yielded 94.90% callus induction.
- Taxon
- cottonGossypium hirsutumt0
- Material
- SVPR-2t0
- Organ / stage
- hypocotyl explante0
- Outcome
- hypocotyl explante0
- Value
- 94.90%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hypocotyl explant (94.90%)”
t0“cotton (Gossypium hirsutum L.) cv. SVPR-2”
Claim · model-written summary
Cotyledon explants yielded 85.20% callus induction.
- Taxon
- cottonGossypium hirsutumt0
- Material
- SVPR-2t0
- Organ / stage
- cotyledon explante0
- Outcome
- cotyledon explante0
- Value
- 85.20%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotyledon explant (85.20%)”
t0“cotton (Gossypium hirsutum L.) cv. SVPR-2”
Claim · model-written summary
MSB5 medium with 2,4-D and 2iP gave strong callus proliferation.
- Taxon
- cottonGossypium hirsutumt0
- Material
- SVPR-2t0
- Treatment
- MSB5 medium supplemented with 2,4-D (0.45 μM) along with 2iP (2.95 μM)e0
- Outcome
- proliferation of calluse0
- Direction
- tremendouse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MSB5 medium supplemented with 2,4-D (0.45 μM) along with 2iP (2.95 μM) gave tremendous proliferation of callus with high percentage of response”
t0“cotton (Gossypium hirsutum L.) cv. SVPR-2”
Claim · model-written summary
Different hormone treatments yielded varying callus colors and textures.
- Taxon
- cottonGossypium hirsutumt0
- Material
- SVPR-2t0
- Treatment
- different hormone treatmentse0
- Outcome
- colors and textures of calluse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Varying degrees of colors and textures of callus were observed under different hormone treatments”
t0“cotton (Gossypium hirsutum L.) cv. SVPR-2”
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