Abstract
Cryopreservation of plum (Prunus domestica L.), cv Regina Claudia, was obtained by a vitrification/one-step cooling procedure of shoot tips from cold-hardened in vitro-grown plants. Best survival (57%) was obtained when the shoot tips were precultured at 4 degree C for 2 days on 0.09 M sucrose-containing Quoirin and Le Poivre medium, loaded for 30 min with a cryoprotectant (2 M glycerol and 0.4 M sucrose), incubated with the PVS2 solution at 0 C for 90 min, and directly plunged into liquid nitrogen. After re-warming in a waterbath at 40 degree C, the shoot tips were washed in a 1.2 M-sucrose MS solution for 20 min and finally plated on a regrowth medium. In comparison with the one-step cooling procedure, both the slow cooling (-0.5 degree C/min up to -45 degree C), and the two-step cooling (-160 degree C for 25 min, then -196 degree C) gave lower percentages of shoot-tip survival. Among the other cryogenic procedures tested, the performance of the encapsulation-vitrification method was similar to the vitrification protocol in terms of shoot-tip regrowth (47.5%), while encapsulation-dehydration was unsatisfactory.
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Claims 5
Claim · model-written summary
The optimized vitrification protocol yielded 57% shoot-tip survival.
- Taxon
- plum
Prunus domesticat0 - Organ / stage
- shoot tipse0
- Treatment
- precultured at 4 degree C for 2 days on 0.09 M sucrose-containing Quoirin and Le Poivre mediume0
- Outcome
- survivale0
- Direction
- Beste0
- Value
- 57%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Best survival (57%) was obtained when the shoot tips were precultured at 4 degree C for 2 days on 0.09 M sucrose-containing Quoirin and Le Poivre medium, loaded for 30 min with a cryoprotectant (2 M glycerol and 0.4 M sucrose), incubated with the PVS2 solution at 0 C for 90 min, and directly plunged into liquid nitrogen.”
t0“plum (Prunus domestica L.)”
Claim · model-written summary
Slow cooling gave lower shoot-tip survival than one-step cooling.
- Taxon
- plumPrunus domesticat0
- Treatment
- slow cooling (-0.5 degree C/min up to -45 degree C)e0
- Outcome
- shoot-tip survivale0
- Direction
- lowere0
- Comparator
- one-step cooling proceduree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In comparison with the one-step cooling procedure, both the slow cooling (-0.5 degree C/min up to -45 degree C), and the two-step cooling (-160 degree C for 25 min, then -196 degree C) gave lower percentages of shoot-tip survival.”
t0“plum (Prunus domestica L.)”
Claim · model-written summary
Two-step cooling gave lower shoot-tip survival than one-step cooling.
- Taxon
- plumPrunus domesticat0
- Treatment
- two-step cooling (-160 degree C for 25 min, then -196 degree C)e0
- Outcome
- shoot-tip survivale0
- Direction
- lowere0
- Comparator
- one-step cooling proceduree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In comparison with the one-step cooling procedure, both the slow cooling (-0.5 degree C/min up to -45 degree C), and the two-step cooling (-160 degree C for 25 min, then -196 degree C) gave lower percentages of shoot-tip survival.”
t0“plum (Prunus domestica L.)”
Claim · model-written summary
Encapsulation-vitrification gave 47.5% shoot-tip regrowth, similar to vitrification.
- Taxon
- plumPrunus domesticat0
- Treatment
- encapsulation-vitrification methode0
- Outcome
- shoot-tip regrowthe0
- Direction
- similare0
- Value
- 47.5%e0
- Comparator
- vitrification protocole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the performance of the encapsulation-vitrification method was similar to the vitrification protocol in terms of shoot-tip regrowth (47.5%)”
t0“plum (Prunus domestica L.)”
Claim · model-written summary
Encapsulation-dehydration gave unsatisfactory performance.
- Taxon
- plumPrunus domesticat0
- Treatment
- encapsulation-dehydratione0
- Outcome
- unsatisfactorye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while encapsulation-dehydration was unsatisfactory.”
t0“plum (Prunus domestica L.)”
The same questions in other papers
in vitro · survival · Prunus domestica (plum)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.