Abstract
Powdery mildew, caused by Podosphaera aphanis, poses a significant threat to strawberry production, while current chemical controls raise environmental and food safety concerns. In this study, we have identified the key regulatory module, FaMYB63/FvWRKY75-PR10.14, that confers enhanced powdery mildew resistance in transgenic strawberry (Fragaria vesca). FaMYB63, an R2R3-MYB transcription factor, was induced by P. aphanis infection and responsive to the application of defence signalling molecules, including salicylic acid (SA), methyl jasmonate (JA), abscisic acid (ABA), and 1-aminocyclopropane-1-carboxylic acid (ACC). Silencing of FaMYB63 led to reduced SA levels and increased powdery mildew susceptibility, accompanied by suppressed reactive oxygen species (ROS) bursts and down-regulation of PR10.14 expression. Conversely, overexpressing PR10.14 in transgenic lines inhibited P. aphanis spore germination and enhanced ROS accumulation, indicating a dual role in direct pathogen inhibition and hypersensitive response-triggered defence. Yeast one-hybrid, electrophoretic mobility shift assay, β-glucuronidase, and luciferase assays confirmed that FaMYB63 and FvWRKY75 were directly bound to the MYB-binding sites and W-box of the PR10.14 promoter, respectively, and activated its transcription, while WRKY75 negatively regulated the expression of MYB63. PR10.14 exhibited tissue-specific expression, with the highest levels in red-ripening fruits, suggesting a role in developmental-stage-dependent defence. These findings suggest FaMYB63 as an SA-dependent regulator of PR10.14-mediated resistance, bridging hormone signalling and pathogen response. This study provides a molecular target for breeding powdery mildew-resistant strawberry cultivars through genetic engineering approaches, offering an alternative to fungicides for sustainable and environmental disease management in horticultural crops and advances our understanding of MYB- or WRKY-PR10P networks in plant immunity.
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Claims 13
Claim · model-written summary
FaMYB63/FvWRKY75-PR10.14 conferred enhanced powdery mildew resistance in transgenic strawberry.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Interactor
- Podosphaera aphanis
Podosphaera aphanise1pathogen - Gene
- FaMYB63/FvWRKY75-PR10.14e0
- Outcome
- powdery mildew resistancee0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“the key regulatory module, FaMYB63/FvWRKY75-PR10.14, that confers enhanced powdery mildew resistance in transgenic strawberry (Fragaria vesca)”
e1“Powdery mildew, caused by Podosphaera aphanis”
Claim · model-written summary
FaMYB63 was induced by P. aphanis infection.
- Taxon
- StrawberryFragaria ×ananassat0
- Interactor
- P. aphanisPodosphaera aphanise0pathogen
- Gene
- FaMYB63e0
- Outcome
- FaMYB63e0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaMYB63, an R2R3-MYB transcription factor, was induced by P. aphanis infection”
t0“Strawberry”
Claim · model-written summary
FaMYB63 responded to defence signalling molecules.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- defence signalling moleculese0
- Gene
- FaMYB63e1
- Outcome
- FaMYB63e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“responsive to the application of defence signalling molecules, including salicylic acid (SA), methyl jasmonate (JA), abscisic acid (ABA), and 1-aminocyclopropane-1-carboxylic acid (ACC)”
t0“Strawberry”
e1“FaMYB63, an R2R3-MYB transcription factor”
Claim · model-written summary
Silencing FaMYB63 reduced salicylic acid levels.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- Silencing of FaMYB63e0
- Gene
- FaMYB63e0
- Outcome
- SA levelse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of FaMYB63 led to reduced SA levels”
t0“Strawberry”
Claim · model-written summary
Silencing FaMYB63 increased powdery mildew susceptibility.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- Silencing of FaMYB63e0
- Gene
- FaMYB63e0
- Outcome
- powdery mildew susceptibilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of FaMYB63 led to reduced SA levels and increased powdery mildew susceptibility”
t0“Strawberry”
Claim · model-written summary
Silencing FaMYB63 suppressed reactive oxygen species bursts.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- Silencing of FaMYB63e0
- Outcome
- reactive oxygen species (ROS) burstse0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of FaMYB63 led to reduced SA levels and increased powdery mildew susceptibility, accompanied by suppressed reactive oxygen species (ROS) bursts”
t0“Strawberry”
Claim · model-written summary
Silencing FaMYB63 downregulated PR10.14 expression.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- Silencing of FaMYB63e0
- Gene
- PR10.14e0
- Outcome
- PR10.14 expressione0
- Direction
- down-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of FaMYB63 led to reduced SA levels and increased powdery mildew susceptibility, accompanied by suppressed reactive oxygen species (ROS) bursts and down-regulation of PR10.14 expression”
t0“Strawberry”
Claim · model-written summary
PR10.14 overexpression inhibited P. aphanis spore germination.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- overexpressing PR10.14e0
- Interactor
- P. aphanisPodosphaera aphanise0pathogen
- Outcome
- P. aphanis spore germinatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpressing PR10.14 in transgenic lines inhibited P. aphanis spore germination”
t0“Strawberry”
Claim · model-written summary
PR10.14 overexpression enhanced ROS accumulation.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- overexpressing PR10.14e0
- Outcome
- ROS accumulatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpressing PR10.14 in transgenic lines inhibited P. aphanis spore germination and enhanced ROS accumulation”
t0“Strawberry”
Claim · model-written summary
FaMYB63 bound the PR10.14 promoter and activated transcription.
- Taxon
- StrawberryFragaria ×ananassat0
- Gene
- FaMYB63e0
- Outcome
- activated its transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaMYB63 and FvWRKY75 were directly bound to the MYB-binding sites and W-box of the PR10.14 promoter, respectively, and activated its transcription”
t0“Strawberry”
Claim · model-written summary
FvWRKY75 bound the PR10.14 promoter and activated transcription.
- Taxon
- StrawberryFragaria ×ananassat0
- Gene
- FvWRKY75e0
- Outcome
- activated its transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaMYB63 and FvWRKY75 were directly bound to the MYB-binding sites and W-box of the PR10.14 promoter, respectively, and activated its transcription”
t0“Strawberry”
Claim · model-written summary
WRKY75 negatively regulated MYB63 expression.
- Taxon
- StrawberryFragaria ×ananassat0
- Gene
- WRKY75e0
- Outcome
- expression of MYB63e0
- Direction
- negatively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WRKY75 negatively regulated the expression of MYB63”
t0“Strawberry”
Claim · model-written summary
PR10.14 expression was highest in red-ripening fruits.
- Taxon
- StrawberryFragaria ×ananassat0
- Organ / stage
- red-ripening fruitse0
- Gene
- PR10.14e0
- Outcome
- PR10.14 exhibited tissue-specific expressione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PR10.14 exhibited tissue-specific expression, with the highest levels in red-ripening fruits”
t0“Strawberry”
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