Abstract
Hemp has a limited planting scope and low and unstable content of nonpsychogenic cannabinoids, and there is a lack of transcriptional regulation research. The mechanism of yeast extract (YE) regulating the synthesis of nonpsychotropic cannabinoids and other terpenoids was clarified through the integration of plant biotechnology and omics. YE (400 mg/L, 96 h) influenced the defense system and enhanced antioxidant activity in hemp adventitious roots (HARs). YE increased cannabinol, tanshinone IIA, dihydrotanshinone I, and trijuganone B contents in HARs from the bioreactor by 231.52%, 205.86%, 300.55%, and 192.59%, respectively. 106 genes and 32 transcription factor families cooperatively regulated terpenoid synthesis. Meanwhile, CsMYC02 and CsMYC12 were speculated to up-regulate the expression of CsDXS2, CsPT5, and CsCBDAS1 within this pathway. This study provides an efficient and green strategy for nonpsychoactive cannabinoid production and enriches the potential functions of the MYC family in response to YE induction, regulating terpenoid synthesis.
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Claims 8
Claim · model-written summary
YE enhanced antioxidant activity and influenced the defense system of hemp adventitious roots.
- Taxon
- hemp
Cannabis sativa subsp. sativae0 - Organ / stage
- adventitious rootse0
- Treatment
- YE (400 mg/L, 96 h)e0
- Outcome
- antioxidant activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“YE (400 mg/L, 96 h) influenced the defense system and enhanced antioxidant activity in hemp adventitious roots (HARs).”
Claim · model-written summary
YE increased cannabinol content by 231.52% in hemp adventitious roots.
- Taxon
- hempCannabis sativa subsp. sativae1
- Treatment
- YEe0
- Outcome
- cannabinole0
- Direction
- increasede0
- Value
- 231.52%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“YE increased cannabinol, tanshinone IIA, dihydrotanshinone I, and trijuganone B contents in HARs from the bioreactor by 231.52%, 205.86%, 300.55%, and 192.59%, respectively.”
e1“hemp adventitious roots (HARs)”
Claim · model-written summary
YE increased tanshinone IIA content by 205.86% in hemp adventitious roots.
- Taxon
- hempCannabis sativa subsp. sativae1
- Treatment
- YEe0
- Outcome
- tanshinone IIAe0
- Direction
- increasede0
- Value
- 205.86%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“YE increased cannabinol, tanshinone IIA, dihydrotanshinone I, and trijuganone B contents in HARs from the bioreactor by 231.52%, 205.86%, 300.55%, and 192.59%, respectively.”
e1“hemp adventitious roots (HARs)”
Claim · model-written summary
YE increased dihydrotanshinone I content by 300.55% in hemp adventitious roots.
- Taxon
- hempCannabis sativa subsp. sativae1
- Treatment
- YEe0
- Outcome
- dihydrotanshinone Ie0
- Direction
- increasede0
- Value
- 300.55%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“YE increased cannabinol, tanshinone IIA, dihydrotanshinone I, and trijuganone B contents in HARs from the bioreactor by 231.52%, 205.86%, 300.55%, and 192.59%, respectively.”
e1“hemp adventitious roots (HARs)”
Claim · model-written summary
YE increased trijuganone B content by 192.59% in hemp adventitious roots.
- Taxon
- hempCannabis sativa subsp. sativae1
- Treatment
- YEe0
- Outcome
- trijuganone Be0
- Direction
- increasede0
- Value
- 192.59%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“YE increased cannabinol, tanshinone IIA, dihydrotanshinone I, and trijuganone B contents in HARs from the bioreactor by 231.52%, 205.86%, 300.55%, and 192.59%, respectively.”
e1“hemp adventitious roots (HARs)”
Claim · model-written summary
The authors report 106 genes and 32 transcription factor families cooperatively regulating terpenoid synthesis.
- Taxon
- hempCannabis sativa subsp. sativae1
- Gene
- genese0
- Outcome
- terpenoid synthesise0
- Value
- 106 genes and 32 transcription factor familiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“106 genes and 32 transcription factor families cooperatively regulated terpenoid synthesis.”
e1“hemp adventitious roots (HARs)”
Claim · model-written summary
CsMYC02 and CsMYC12 were proposed to upregulate CsDXS2, CsPT5 and CsCBDAS1 expression.
- Taxon
- hempCannabis sativa subsp. sativae1
- Gene
- CsMYC02 and CsMYC12e0
- Outcome
- expression of CsDXS2, CsPT5, and CsCBDAS1e0
- Direction
- up-regulatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsMYC02 and CsMYC12 were speculated to up-regulate the expression of CsDXS2, CsPT5, and CsCBDAS1 within this pathway.”
e1“hemp adventitious roots (HARs)”
Claim · model-written summary
The authors present yeast extract induction as an efficient green strategy for nonpsychoactive cannabinoid production.
- Taxon
- hempCannabis sativa subsp. sativae1
- Outcome
- efficient and green strategy for nonpsychoactive cannabinoid productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study provides an efficient and green strategy for nonpsychoactive cannabinoid production”
e1“hemp adventitious roots (HARs)”
The same questions in other papers
botanical · molecular: metabolites · Cannabis sativaPositive and negative results
1 in the opposite direction
- negative
botanical · quality: phytochemicals · Cannabis sativaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.