Abstract
Selenium (Se) is an essential micronutrient for human health, yet its dietary intake is insufficient in many populations worldwide. Agronomic biofortification represents an effective strategy to enrich crops with Se, and Totally Controlled Environment Agriculture (TCEA) provides a reliable platform to evaluate cultivar-specific responses under standardized conditions. This study evaluated the effects of foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars, ‘Fine Verde’ (FV) and ‘Red Rubin’ (RR), in a micro-TCEA system. The yield was not significantly different at 5–10 µM but declined by 21% at 15 µM, particularly for FV. RR out-yielded FV (+14%), whereas FV produced taller shoots. The 5 µM Se concentration did not affect the total chlorophyll content and quantum yield of photosystem II under control conditions. The highest Se dose (15 µM) decreased the chlorophyll content and electron transport rate by 18% and 12%, respectively, while increasing the stomatal conductance (50%) and transpiration rate (120%). The total phenolics content in RR was double that in FV and increased with Se, whereas the NO3− concentration in RR decreased by 9% at 10 µM. Principal component analysis separated treatments by Se dose (PC1 = 44.5%) and cultivar (PC2 = 42.7%), showing RR’s stronger connection of RR to biomass and antioxidant accumulation under moderate Se. Overall, a single foliar application of 5 µM sodium selenate appears optimal to achieve effective Se enrichment while maintaining productivity and quality. These findings support basil as a promising candidate for Se biofortification in TCEA systems, with potential contributions to dietary Se intake.
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Claims 15
Claim · model-written summary
Basil yield was not significantly different at foliar selenate doses of 5-10 µM.
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Treatment
- foliar sodium selenate doses of 0, 5, 10, and 15 µMe0
- Outcome
- yielde1
- Direction
- not significantly differente1
- Qualifier
- 5–10 µMe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“The yield was not significantly different at 5–10 µM but declined by 21% at 15 µM”
Claim · model-written summary
Basil yield declined by 21% at 15 µM selenate, particularly for FV.
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Treatment
- foliar sodium selenate doses of 0, 5, 10, and 15 µMe0
- Outcome
- yielde1
- Direction
- declinede1
- Value
- 21%e1
- Qualifier
- 15 µMe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“The yield was not significantly different at 5–10 µM but declined by 21% at 15 µM”
Claim · model-written summary
Cultivar RR out-yielded FV by 14%.
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Material
- RRe1
- Outcome
- out-yieldede1
- Direction
- out-yieldede1
- Value
- +14%e1
- Comparator
- FVe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“RR out-yielded FV (+14%)”
Claim · model-written summary
Cultivar FV produced taller shoots.
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Material
- FVe1
- Outcome
- taller shootse1
- Direction
- tallere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“whereas FV produced taller shoots”
Claim · model-written summary
Under control conditions, 5 µM Se did not affect total chlorophyll content or quantum yield of photosystem II.
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Treatment
- 5 µM Se concentratione1
- Outcome
- total chlorophyll content and quantum yield of photosystem IIe1
- Direction
- did not affecte1
- Setting
- control conditionse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“The 5 µM Se concentration did not affect the total chlorophyll content and quantum yield of photosystem II under control conditions”
Claim · model-written summary
The highest Se dose (15 µM) changed basil chlorophyll content (decreased, 18%).
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Treatment
- highest Se dose (15 µM)e1
- Outcome
- chlorophyll contente1
- Direction
- decreasede1
- Value
- 18%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“The highest Se dose (15 µM) decreased the chlorophyll content and electron transport rate by 18% and 12%, respectively, while increasing the stomatal conductance (50%) and transpiration rate (120%)”
Claim · model-written summary
The highest Se dose (15 µM) changed basil electron transport rate (decreased, 12%).
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Treatment
- highest Se dose (15 µM)e1
- Outcome
- electron transport ratee1
- Direction
- decreasede1
- Value
- 12%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“The highest Se dose (15 µM) decreased the chlorophyll content and electron transport rate by 18% and 12%, respectively, while increasing the stomatal conductance (50%) and transpiration rate (120%)”
Claim · model-written summary
The highest Se dose (15 µM) changed basil stomatal conductance (increasing, 50%).
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Treatment
- highest Se dose (15 µM)e1
- Outcome
- stomatal conductancee1
- Direction
- increasinge1
- Value
- 50%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“The highest Se dose (15 µM) decreased the chlorophyll content and electron transport rate by 18% and 12%, respectively, while increasing the stomatal conductance (50%) and transpiration rate (120%)”
Claim · model-written summary
The highest Se dose (15 µM) changed basil transpiration rate (increasing, 120%).
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Treatment
- highest Se dose (15 µM)e1
- Outcome
- transpiration ratee1
- Direction
- increasinge1
- Value
- 120%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“The highest Se dose (15 µM) decreased the chlorophyll content and electron transport rate by 18% and 12%, respectively, while increasing the stomatal conductance (50%) and transpiration rate (120%)”
Claim · model-written summary
Total phenolics content in RR was double that in FV.
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Material
- RRe1
- Outcome
- total phenolics contente1
- Direction
- doublee1
- Comparator
- FVe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“The total phenolics content in RR was double that in FV and increased with Se”
Claim · model-written summary
Total phenolics content in RR increased with Se.
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Material
- RRe1
- Treatment
- See1
- Outcome
- total phenolics contente1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“The total phenolics content in RR was double that in FV and increased with Se”
Claim · model-written summary
NO3- concentration in RR decreased by 9% at 10 µM selenate.
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Material
- RRe1
- Treatment
- foliar sodium selenate doses of 0, 5, 10, and 15 µMe0
- Outcome
- NO3− concentratione1
- Direction
- decreasede1
- Value
- 9%e1
- Qualifier
- 10 µMe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“the NO3− concentration in RR decreased by 9% at 10 µM”
Claim · model-written summary
Principal component analysis separated treatments by Se dose (PC1 = 44.5%) and cultivar (PC2 = 42.7%).
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Outcome
- separated treatmentse1
- Value
- PC1 = 44.5%e1
- Qualifier
- PC2 = 42.7%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“Principal component analysis separated treatments by Se dose (PC1 = 44.5%) and cultivar (PC2 = 42.7%)”
Claim · model-written summary
RR showed a stronger connection to biomass and antioxidant accumulation under moderate Se.
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Material
- RRe1
- Treatment
- moderate See1
- Outcome
- biomass and antioxidant accumulatione1
- Direction
- stronger connectione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“RR’s stronger connection of RR to biomass and antioxidant accumulation under moderate Se”
Claim · model-written summary
A single foliar application of 5 µM sodium selenate appears optimal for Se enrichment while maintaining productivity and quality.
- Taxon
- Ocimum basilicum L.
Ocimum basilicumt0 - Outcome
- optimale1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Ocimum basilicum L.”
e0“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”
e1“a single foliar application of 5 µM sodium selenate appears optimal to achieve effective Se enrichment while maintaining productivity and quality”
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6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.