Paper · 2025 · Agronomy

Foliar Biofortification with Sodium Selenate Enhances Selenium Content in Ocimum basilicum L. Cultivars in a Totally Controlled Environment System

Cosimo Profico; Saeed Fattahi Siah Kamari; Vali Rabiei; et al.

15
claims
45
verbatim quotes
2
citations
2
research areas
✓
Open access · cc-by

Abstract

Selenium (Se) is an essential micronutrient for human health, yet its dietary intake is insufficient in many populations worldwide. Agronomic biofortification represents an effective strategy to enrich crops with Se, and Totally Controlled Environment Agriculture (TCEA) provides a reliable platform to evaluate cultivar-specific responses under standardized conditions. This study evaluated the effects of foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars, ‘Fine Verde’ (FV) and ‘Red Rubin’ (RR), in a micro-TCEA system. The yield was not significantly different at 5–10 µM but declined by 21% at 15 µM, particularly for FV. RR out-yielded FV (+14%), whereas FV produced taller shoots. The 5 µM Se concentration did not affect the total chlorophyll content and quantum yield of photosystem II under control conditions. The highest Se dose (15 µM) decreased the chlorophyll content and electron transport rate by 18% and 12%, respectively, while increasing the stomatal conductance (50%) and transpiration rate (120%). The total phenolics content in RR was double that in FV and increased with Se, whereas the NO3− concentration in RR decreased by 9% at 10 µM. Principal component analysis separated treatments by Se dose (PC1 = 44.5%) and cultivar (PC2 = 42.7%), showing RR’s stronger connection of RR to biomass and antioxidant accumulation under moderate Se. Overall, a single foliar application of 5 µM sodium selenate appears optimal to achieve effective Se enrichment while maintaining productivity and quality. These findings support basil as a promising candidate for Se biofortification in TCEA systems, with potential contributions to dietary Se intake.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 15

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Basil yield was not significantly different at foliar selenate doses of 5-10 µM.

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Treatment
foliar sodium selenate doses of 0, 5, 10, and 15 µMe0
Outcome
yielde1
Direction
not significantly differente1
Qualifier
5–10 µMe1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“The yield was not significantly different at 5–10 µM but declined by 21% at 15 µM”

✓ located verbatim in the source · abstract, characters 569–650 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Basil yield declined by 21% at 15 µM selenate, particularly for FV.

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Treatment
foliar sodium selenate doses of 0, 5, 10, and 15 µMe0
Outcome
yielde1
Direction
declinede1
Value
21%e1
Qualifier
15 µMe1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“The yield was not significantly different at 5–10 µM but declined by 21% at 15 µM”

✓ located verbatim in the source · abstract, characters 569–650 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Cultivar RR out-yielded FV by 14%.

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Material
RRe1
Outcome
out-yieldede1
Direction
out-yieldede1
Value
+14%e1
Comparator
FVe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“RR out-yielded FV (+14%)”

✓ located verbatim in the source · abstract, characters 673–697 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Cultivar FV produced taller shoots.

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Material
FVe1
Outcome
taller shootse1
Direction
tallere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“whereas FV produced taller shoots”

✓ located verbatim in the source · abstract, characters 699–732 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Under control conditions, 5 µM Se did not affect total chlorophyll content or quantum yield of photosystem II.

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Treatment
5 µM Se concentratione1
Outcome
total chlorophyll content and quantum yield of photosystem IIe1
Direction
did not affecte1
Setting
control conditionse1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“The 5 µM Se concentration did not affect the total chlorophyll content and quantum yield of photosystem II under control conditions”

✓ located verbatim in the source · abstract, characters 734–865 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The highest Se dose (15 µM) changed basil chlorophyll content (decreased, 18%).

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Treatment
highest Se dose (15 µM)e1
Outcome
chlorophyll contente1
Direction
decreasede1
Value
18%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“The highest Se dose (15 µM) decreased the chlorophyll content and electron transport rate by 18% and 12%, respectively, while increasing the stomatal conductance (50%) and transpiration rate (120%)”

✓ located verbatim in the source · abstract, characters 867–1064 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The highest Se dose (15 µM) changed basil electron transport rate (decreased, 12%).

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Treatment
highest Se dose (15 µM)e1
Outcome
electron transport ratee1
Direction
decreasede1
Value
12%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“The highest Se dose (15 µM) decreased the chlorophyll content and electron transport rate by 18% and 12%, respectively, while increasing the stomatal conductance (50%) and transpiration rate (120%)”

✓ located verbatim in the source · abstract, characters 867–1064 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The highest Se dose (15 µM) changed basil stomatal conductance (increasing, 50%).

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Treatment
highest Se dose (15 µM)e1
Outcome
stomatal conductancee1
Direction
increasinge1
Value
50%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“The highest Se dose (15 µM) decreased the chlorophyll content and electron transport rate by 18% and 12%, respectively, while increasing the stomatal conductance (50%) and transpiration rate (120%)”

✓ located verbatim in the source · abstract, characters 867–1064 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The highest Se dose (15 µM) changed basil transpiration rate (increasing, 120%).

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Treatment
highest Se dose (15 µM)e1
Outcome
transpiration ratee1
Direction
increasinge1
Value
120%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“The highest Se dose (15 µM) decreased the chlorophyll content and electron transport rate by 18% and 12%, respectively, while increasing the stomatal conductance (50%) and transpiration rate (120%)”

✓ located verbatim in the source · abstract, characters 867–1064 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Total phenolics content in RR was double that in FV.

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Material
RRe1
Outcome
total phenolics contente1
Direction
doublee1
Comparator
FVe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“The total phenolics content in RR was double that in FV and increased with Se”

✓ located verbatim in the source · abstract, characters 1066–1143 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Total phenolics content in RR increased with Se.

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Material
RRe1
Treatment
See1
Outcome
total phenolics contente1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“The total phenolics content in RR was double that in FV and increased with Se”

✓ located verbatim in the source · abstract, characters 1066–1143 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

NO3- concentration in RR decreased by 9% at 10 µM selenate.

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Material
RRe1
Treatment
foliar sodium selenate doses of 0, 5, 10, and 15 µMe0
Outcome
NO3− concentratione1
Direction
decreasede1
Value
9%e1
Qualifier
10 µMe1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“the NO3− concentration in RR decreased by 9% at 10 µM”

✓ located verbatim in the source · abstract, characters 1153–1206 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Principal component analysis separated treatments by Se dose (PC1 = 44.5%) and cultivar (PC2 = 42.7%).

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Outcome
separated treatmentse1
Value
PC1 = 44.5%e1
Qualifier
PC2 = 42.7%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“Principal component analysis separated treatments by Se dose (PC1 = 44.5%) and cultivar (PC2 = 42.7%)”

✓ located verbatim in the source · abstract, characters 1208–1309 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

RR showed a stronger connection to biomass and antioxidant accumulation under moderate Se.

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Material
RRe1
Treatment
moderate See1
Outcome
biomass and antioxidant accumulatione1
Direction
stronger connectione1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“RR’s stronger connection of RR to biomass and antioxidant accumulation under moderate Se”

✓ located verbatim in the source · abstract, characters 1319–1407 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

A single foliar application of 5 µM sodium selenate appears optimal for Se enrichment while maintaining productivity and quality.

Taxon
Ocimum basilicum L.Ocimum basilicumt0
Outcome
optimale1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Ocimum basilicum L.”

✓ located verbatim in the source · title, characters 74–93 · show in text ↑
e0

“foliar sodium selenate doses of 0, 5, 10, and 15 µM on two basil (Ocimum basilicum L.) cultivars”

✓ located verbatim in the source · abstract, characters 407–503 · show in text ↑
e1

“a single foliar application of 5 µM sodium selenate appears optimal to achieve effective Se enrichment while maintaining productivity and quality”

✓ located verbatim in the source · abstract, characters 1418–1563 · show in text ↑

selenium atom · physiology: photosynthesis · Ocimum basilicum (basil)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

selenium atom · physiology: pigments · Ocimum basilicum (basil)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. no effect
See all 4 papers →

application · yield · Ocimum basilicum (basil)Positive and negative results

10 papers: 9 positive, 1 negative or no effect

9 in the opposite direction

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.