Abstract
Soybean (Glycine max L.) is an essential oilseed crop widely recognised for its high protein and oil content. Soybean (Co1) is a high-yielding variety and well-suited for cultivation in Tamil Nadu and nearby regions. Direct and indirect organogenesis of soybean using different growth regulators is helpful for regeneration, micropropagation and genetic transformation. We evaluated the impact of various plant growth regulators (BAP, KIN, ZT and TDZ) for direct organogenesis using split seeds and cotyledonary nodes of soybean (Co1). Among the different combinations, B5 medium with BAP 1.0 mg/L (for multiple shoots) and KIN 1.0 mg/L (for shoot length) revealed the better outcomes for the direct shoot rejuvenation in the split-seeds. The highest frequency of multiple shoots from the cotyledonary node was induced in MS medium containing TDZ at 0.5 mg/L. Different levels of 2,4-D have been assessed for callus induction from various explants and a higher percentage of callus was developed in hypocotyls. MS media containing 2,4-D (2.0 mg/L) and KIN (0.2 mg/L) leads to better callus development and the friable callus was further used for shoot induction using BAP, TDZ and ZT. After 2 - 3 weeks, initiation of shoot formation from embryonic callus was observed in MS medium supplemented with 1.0 mg/L ZT and 0.5 mg/L IAA. Shoot induction from split seeds showed better response than the cotyledonary node and callus. These findings are expected to be useful for the regeneration of soybean (Co1) to develop transgenic lines for enrichment of nutritional value.
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Claims 7
Claim · model-written summary
B5 medium with BAP 1.0 mg/L gave better multiple shoot formation in split seeds.
- Taxon
- soybean
Glycine maxe1 - Organ / stage
- split-seedse0
- Treatment
- B5 medium with BAP 1.0 mg/Le0
- Outcome
- multiple shootse0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B5 medium with BAP 1.0 mg/L (for multiple shoots) and KIN 1.0 mg/L (for shoot length) revealed the better outcomes for the direct shoot rejuvenation in the split-seeds”
e1“cotyledonary nodes of soybean (Co1)”
Claim · model-written summary
KIN 1.0 mg/L improved shoot length in split seeds.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- split-seedse0
- Treatment
- KIN 1.0 mg/Le0
- Outcome
- shoot lengthe0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“KIN 1.0 mg/L (for shoot length) revealed the better outcomes for the direct shoot rejuvenation in the split-seeds”
e1“cotyledonary nodes of soybean (Co1)”
Claim · model-written summary
MS medium with TDZ 0.5 mg/L induced the highest frequency of multiple shoots from cotyledonary nodes.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- cotyledonary nodee0
- Treatment
- MS medium containing TDZ at 0.5 mg/Le0
- Outcome
- frequency of multiple shootse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest frequency of multiple shoots from the cotyledonary node was induced in MS medium containing TDZ at 0.5 mg/L”
e1“cotyledonary nodes of soybean (Co1)”
Claim · model-written summary
Hypocotyls produced a higher percentage of callus.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- hypocotylse0
- Outcome
- percentage of calluse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a higher percentage of callus was developed in hypocotyls”
e1“cotyledonary nodes of soybean (Co1)”
Claim · model-written summary
MS medium with 2,4-D 2.0 mg/L and KIN 0.2 mg/L gave better callus development.
- Taxon
- soybeanGlycine maxe1
- Treatment
- MS media containing 2,4-D (2.0 mg/L) and KIN (0.2 mg/L)e0
- Outcome
- callus developmente0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS media containing 2,4-D (2.0 mg/L) and KIN (0.2 mg/L) leads to better callus development”
e1“cotyledonary nodes of soybean (Co1)”
Claim · model-written summary
Embryonic callus initiated shoots after 2–3 weeks on MS medium with ZT and IAA.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- embryonic calluse0
- Treatment
- MS medium supplemented with 1.0 mg/L ZT and 0.5 mg/L IAAe0
- Outcome
- initiation of shoot formatione0
- Value
- 2 - 3 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 2 - 3 weeks, initiation of shoot formation from embryonic callus was observed in MS medium supplemented with 1.0 mg/L ZT and 0.5 mg/L IAA”
e1“cotyledonary nodes of soybean (Co1)”
Claim · model-written summary
Split seeds responded better for shoot induction than cotyledonary nodes and callus.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- split seedse0
- Outcome
- Shoot inductione0
- Direction
- bettere0
- Comparator
- cotyledonary node and calluse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot induction from split seeds showed better response than the cotyledonary node and callus”
e1“cotyledonary nodes of soybean (Co1)”
The same questions in other papers
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See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.