Abstract
Abstract Low-temperature stress, which reduces the productivity of cultivated plants and their cultivation potential in temperate climate zones, is accompanied by oxidative stress. The reduction of ROS generation in plant cells can be achieved, among other methods, by activating alternative electron transport pathways in mitochondria. The aim of the study was to assess the influence of the heterologous AtNDB2 (mitochondrial external alternative NADH dehydrogenase from Arabidopsis thaliana L. Heynh.) gene expression on the functional state of the photosynthetic apparatus, the level of ROS generation, the expression of genes encoding enzymes that form the mitochondrial alternative electron transport pathway, and the superoxide dismutase (SOD) activity under low positive temperature stress in tobacco plants (Nicotiana tabacum L.). It was shown that in the leaves of transgenic plants (13S line) under low-temperature stress, the decrease in the maximum quantum yield of PSII was less than in wild type (WT) plants. Also higher value of the non-photochemical quenching of chlorophyll fluorescence (NPQ) in the leaves of 13S line was detected. A decrease in the ROS production in the leaves of 13S plants under low-temperature stress was observed. AtNDB2 heterologous expression was accompanied by an increase in the number of mRNA of genes encoding other mitochondrial alternative electron transport pathway components—NDA2 and AOX1a. This fact confirms the possibility of forming this alternative pathway in the mitochondria of 13S plants—NDB2/NDA2–Q/QH2–AOX. Under control conditions, AtNDB2 expression led to a slight Mn-SOD activity increase. Under cold stress, the different SOD isoforms activity in the 13S plant leaves was lower than in WT plants.
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Claims 7
Claim · model-written summary
Under low-temperature stress, the decrease in maximum quantum yield of PSII in 13S transgenic plants was less than in wild type.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- 13S linee1
- Treatment
- low-temperature stresse1
- Outcome
- maximum quantum yield of PSIIe1
- Direction
- lesse1
- Comparator
- wild type (WT) plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco plants (Nicotiana tabacum L.)”
e1“in the leaves of transgenic plants (13S line) under low-temperature stress, the decrease in the maximum quantum yield of PSII was less than in wild type (WT) plants”
Claim · model-written summary
The 13S line showed higher non-photochemical quenching of chlorophyll fluorescence (NPQ) in its leaves.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- 13S linee2
- Outcome
- non-photochemical quenching of chlorophyll fluorescence (NPQ)e2
- Direction
- highere2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco plants (Nicotiana tabacum L.)”
e2“Also higher value of the non-photochemical quenching of chlorophyll fluorescence (NPQ) in the leaves of 13S line was detected”
Claim · model-written summary
ROS production decreased in 13S plant leaves under low-temperature stress.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- 13S plantse3
- Treatment
- low-temperature stresse3
- Outcome
- ROS productione3
- Direction
- decreasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco plants (Nicotiana tabacum L.)”
e3“A decrease in the ROS production in the leaves of 13S plants under low-temperature stress was observed”
Claim · model-written summary
AtNDB2 expression increased the mRNA numbers of the genes NDA2 and AOX1a.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- AtNDB2 heterologous expressione4
- Gene
- NDA2 and AOX1ae5
- Outcome
- number of mRNA of genese4
- Direction
- increasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco plants (Nicotiana tabacum L.)”
e4“AtNDB2 heterologous expression was accompanied by an increase in the number of mRNA of genes encoding other mitochondrial alternative electron transport pathway components”
e5“NDA2 and AOX1a”
Claim · model-written summary
The authors interpret the AtNDB2 result as confirming the possibility of an NDB2/NDA2-Q/QH2-AOX alternative pathway in 13S mitochondria.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- 13S plantse6
- Outcome
- possibility of forming this alternative pathwaye6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco plants (Nicotiana tabacum L.)”
e6“This fact confirms the possibility of forming this alternative pathway in the mitochondria of 13S plants”
Claim · model-written summary
Under control conditions, AtNDB2 expression slightly increased Mn-SOD activity.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- AtNDB2 expressione7
- Outcome
- Mn-SOD activitye7
- Direction
- increasee7
- Qualifier
- Under control conditionse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco plants (Nicotiana tabacum L.)”
e7“Under control conditions, AtNDB2 expression led to a slight Mn-SOD activity increase”
Claim · model-written summary
Under cold stress, SOD isoform activity in 13S plant leaves was lower than in wild type.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- 13S plante7
- Treatment
- cold stresse7
- Outcome
- SOD isoforms activitye7
- Direction
- lowere7
- Comparator
- WT plantse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco plants (Nicotiana tabacum L.)”
e7“Under cold stress, the different SOD isoforms activity in the 13S plant leaves was lower than in WT plants”
The same questions in other papers
cold · physiology: oxidative · Nicotiana tabacum (tobacco)Results differ
6 papers in the same direction as this paper · 8 in the opposite direction
- negative
- negative
- negative
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.