Abstract
A short heat pre-treatment (1 h at 38°C) was found to protect both suspension-cultured apple fruit cells and tobacco cells from cold-induced cell death. Tobacco cells were more sensitive to low temperatures than apple cells, with significant cell death after 48 h at 0 or –2°C. Real-time measurements of H2O2 levels during the heat pre-treatment revealed a substantial burst of this reactive oxygen species in both cell types. Real-time and longer-term measurements also showed a large burst of H2O2 production from tobacco cells, but not apple cells, when exposed to low temperatures. Lower temperatures reduced levels of peroxidase activity (both total and intracellular), with the heat pre-treatment preventing some of the cold-induced reduction of this activity in both apple and tobacco cells. The greater sensitivity to low temperature of the tobacco cells may be related to higher H2O2 production, with the heat treatment maintaining higher peroxidase activity. The lesser sensitivity of the apple cells may be due to the lack of a H2O2 burst and maintenance of peroxidase activity by the heat treatment. These results support a role for oxidative metabolism in the beneficial effects of heat in inducing low temperature tolerance.
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Claims 11
Claim · model-written summary
Heat pretreatment protected apple fruit cells from cold induced death.
- Taxon
- apple
Malus domesticae0 - Organ / stage
- fruit cellse0
- Treatment
- heat pre-treatment (1 h at 38°C)e0
- Outcome
- cold-induced cell deathe0
- Direction
- protecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A short heat pre-treatment (1 h at 38°C) was found to protect both suspension-cultured apple fruit cells and tobacco cells from cold-induced cell death.”
Claim · model-written summary
Heat pretreatment protected tobacco cells from cold induced death.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- heat pre-treatment (1 h at 38°C)e0
- Outcome
- cold-induced cell deathe0
- Direction
- protecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A short heat pre-treatment (1 h at 38°C) was found to protect both suspension-cultured apple fruit cells and tobacco cells from cold-induced cell death.”
Claim · model-written summary
Tobacco cells were more sensitive to low temperatures than apple cells, with significant death after 48 hours at 0 or minus 2 degrees C.
- Taxon
- TobaccoNicotiana tabacume0
- Outcome
- cell deathe0
- Direction
- more sensitivee0
- Qualifier
- after 48 h at 0 or –2°Ce0
- Comparator
- apple cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tobacco cells were more sensitive to low temperatures than apple cells, with significant cell death after 48 h at 0 or –2°C.”
Claim · model-written summary
Heat pretreatment caused a substantial hydrogen peroxide burst in apple cells.
- Taxon
- appleMalus domesticae1
- Treatment
- heat pre-treatmente0
- Outcome
- H2O2 levelse0
- Direction
- burste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Real-time measurements of H2O2 levels during the heat pre-treatment revealed a substantial burst of this reactive oxygen species in both cell types.”
e1“apple fruit cells”
Claim · model-written summary
Heat pretreatment caused a substantial hydrogen peroxide burst in tobacco cells.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- heat pre-treatmente0
- Outcome
- H2O2 levelse0
- Direction
- burste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Real-time measurements of H2O2 levels during the heat pre-treatment revealed a substantial burst of this reactive oxygen species in both cell types.”
e1“Tobacco cells were more sensitive”
Claim · model-written summary
Low temperatures caused a large hydrogen peroxide production burst in tobacco cells.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- low temperaturese0
- Outcome
- H2O2 productione0
- Direction
- burste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a large burst of H2O2 production from tobacco cells, but not apple cells, when exposed to low temperatures”
Claim · model-written summary
Apple cells did not show a hydrogen peroxide production burst under low temperatures.
- Taxon
- appleMalus domesticae0
- Treatment
- low temperaturese0
- Outcome
- H2O2 productione0
- Direction
- note0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a large burst of H2O2 production from tobacco cells, but not apple cells, when exposed to low temperatures”
Claim · model-written summary
Lower temperatures reduced total and intracellular peroxidase activity in apple cells.
- Taxon
- appleMalus domesticae1
- Treatment
- Lower temperaturese0
- Outcome
- peroxidase activity (both total and intracellular)e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lower temperatures reduced levels of peroxidase activity (both total and intracellular), with the heat pre-treatment preventing some of the cold-induced reduction of this activity in both apple and tobacco cells.”
e1“apple fruit cells”
Claim · model-written summary
Lower temperatures reduced total and intracellular peroxidase activity in tobacco cells.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Lower temperaturese0
- Outcome
- peroxidase activity (both total and intracellular)e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lower temperatures reduced levels of peroxidase activity (both total and intracellular), with the heat pre-treatment preventing some of the cold-induced reduction of this activity in both apple and tobacco cells.”
Claim · model-written summary
Heat pretreatment prevented some cold induced reduction of peroxidase activity in apple cells.
- Taxon
- appleMalus domesticae0
- Treatment
- heat pre-treatmente0
- Outcome
- cold-induced reduction of this activitye0
- Direction
- preventinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heat pre-treatment preventing some of the cold-induced reduction of this activity in both apple and tobacco cells.”
Claim · model-written summary
Heat pretreatment prevented some cold induced reduction of peroxidase activity in tobacco cells.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- heat pre-treatmente0
- Outcome
- cold-induced reduction of this activitye0
- Direction
- preventinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heat pre-treatment preventing some of the cold-induced reduction of this activity in both apple and tobacco cells.”
The same questions in other papers
cold · physiology: oxidative · Nicotiana tabacum (tobacco)Results differ
7 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →heat · cytology · Nicotiana tabacum (tobacco)Positive and negative results
3 in the opposite direction
- negative
- negative
- negative
heat · cytology · Malus domestica (apple)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.