Abstract
The long-term application of traditional chemical herbicides has caused a significant escalation in herbicide resistance of barnyard grass (Echinochloa crus-galli). As an eco-friendly alternative, biological herbicides demonstrate substantial application potential. Acknowledging the growing herbicide resistance of E. crus-galli, this study aimed to screen target bacteria with inhibitory effects on the growth for bio-herbicide development. By using ungerminated E. crus-galli seeds as the screening substrate, a bacterial strain (BFYBC-8) with potent inhibitory activity was isolated and identified as Brucella pseudorignonensis. Pot experiments revealed that inoculation with B. pseudorignonensis BFYBC-8 significantly suppressed E. crus-galli growth, reducing plant height by 16.7% and root length by 85.1%, while markedly inhibiting biomass accumulation. Fluorescent labeling with green fluorescent protein (GFP) showed that BFYBC-8 successfully colonized the root intercellular spaces of E. crus-galli and extended continuously along the tissue matrix. Additionally, the strain’s supernatant metabolic products exhibited exceptional thermostability: inhibitory activity against E. crus-galli was maintained after thermal treatment at 28 °C, 60 °C, 80 °C, and 100 °C. Crucially, the bacterium displayed no toxicity to agronomically important crops such as rice, wheat, and corn. This study highlights B. pseudorignonensis BFYBC-8 as a promising candidate for bioherbicide development and provides an important reference for applying seed-associated pathogenic bacteria in developing bioherbicides for sustainable weed management.
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Claims 7
Claim · model-written summary
BFYBC-8 was isolated from ungerminated seeds and identified as Brucella pseudorignonensis.
- Taxon
- E. crus-galli
Echinochloa crus-gallie0 - Interactor
- Brucella pseudorignonensise0biocontrol_agent
- Outcome
- a bacterial strain (BFYBC-8) with potent inhibitory activity was isolated and identified as Brucella pseudorignonensise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By using ungerminated E. crus-galli seeds as the screening substrate, a bacterial strain (BFYBC-8) with potent inhibitory activity was isolated and identified as Brucella pseudorignonensis”
Claim · model-written summary
BFYBC-8 reduced barnyard grass height by 16.7%.
- Taxon
- E. crus-galliEchinochloa crus-gallie0
- Treatment
- inoculation with B. pseudorignonensis BFYBC-8e0
- Interactor
- B. pseudorignonensis BFYBC-8e0biocontrol_agent
- Outcome
- plant heighte0
- Direction
- reducinge0
- Value
- 16.7%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inoculation with B. pseudorignonensis BFYBC-8 significantly suppressed E. crus-galli growth, reducing plant height by 16.7%”
Claim · model-written summary
BFYBC-8 reduced barnyard grass root length by 85.1%.
- Taxon
- E. crus-galliEchinochloa crus-gallie0
- Treatment
- inoculation with B. pseudorignonensis BFYBC-8e0
- Interactor
- B. pseudorignonensis BFYBC-8e0biocontrol_agent
- Outcome
- root lengthe0
- Direction
- reducinge0
- Value
- 85.1%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inoculation with B. pseudorignonensis BFYBC-8 significantly suppressed E. crus-galli growth, reducing plant height by 16.7% and root length by 85.1%”
Claim · model-written summary
BFYBC-8 inhibited barnyard grass biomass accumulation.
- Taxon
- E. crus-galliEchinochloa crus-gallie0
- Treatment
- inoculation with B. pseudorignonensis BFYBC-8e0
- Interactor
- B. pseudorignonensis BFYBC-8e0biocontrol_agent
- Outcome
- biomass accumulatione0
- Direction
- inhibitinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inoculation with B. pseudorignonensis BFYBC-8 significantly suppressed E. crus-galli growth, reducing plant height by 16.7% and root length by 85.1%, while markedly inhibiting biomass accumulation”
Claim · model-written summary
BFYBC-8 colonized root intercellular spaces and extended along the tissue matrix.
- Taxon
- E. crus-galliEchinochloa crus-gallie0
- Interactor
- BFYBC-8e0biocontrol_agent
- Outcome
- colonized the root intercellular spacese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BFYBC-8 successfully colonized the root intercellular spaces of E. crus-galli and extended continuously along the tissue matrix”
Claim · model-written summary
Supernatant inhibition persisted after heating from 28 to 100 °C.
- Taxon
- E. crus-galliEchinochloa crus-gallie0
- Outcome
- inhibitory activity against E. crus-gallie0
- Qualifier
- thermal treatment at 28 °C, 60 °C, 80 °C, and 100 °Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibitory activity against E. crus-galli was maintained after thermal treatment at 28 °C, 60 °C, 80 °C, and 100 °C”
Claim · model-written summary
The bacterium showed no toxicity to rice, wheat, and corn.
- Taxon
- rice, wheat, and corn
Oryza sativa
Triticum aestivum
Zea mays subsp. mayse0 - Outcome
- no toxicitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the bacterium displayed no toxicity to agronomically important crops such as rice, wheat, and corn”
The same questions in other papers
inoculation · growth · Echinochloa crus-galli (barnyard grass)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.