Abstract
All organisms experience stress as an inevitable part of life, from single-celled microorganisms to complex multicellular beings. The ability to recover from stress is a fundamental trait that determines the overall resilience of an organism, yet stress recovery is understudied. To investigate how plants recover from drought, we examine a fine-scale time series of RNA sequencing starting 15 min after rehydration following moderate drought. We reveal that drought recovery is a rapid process involving the activation of thousands of recovery-specific genes. To capture these rapid recovery responses in different Arabidopsis thaliana (A. thaliana) leaf cell types, we perform a single-nucleus transcriptome analysis at the onset of drought recovery, identifying a cell type-specific transcriptional state developing independently across cell types. To further validate the cell-type specific transcriptional changes observed during drought recovery, we employ spatial transcriptomics using multiplexed error-robust fluorescence in situ hybridization (MERFISH), revealing anatomical localization of recovery-induced gene expression programs across Arabidopsis leaf tissues. Furthermore, we reveal a recovery-induced activation of the immune system that occurs autonomously, and which enhances pathogen resistance in vivo in A. thaliana, wild tomato (Solanum pennellii) and domesticated tomato (Solanum lycopersicum cv. M82). Since rehydration promotes microbial proliferation and thereby increases the risk of infection, the activation of drought recovery-induced immunity may be crucial for plant survival in natural environments. These findings indicate that drought recovery coincides with a preventive defense response, unraveling the complex regulatory mechanisms that facilitate stress recovery in different plant cell types.
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Claims 7
Claim · model-written summary
Drought recovery activated thousands of recovery-specific genes.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Outcome
- recovery-specific genese0
- Direction
- activatione0
- Value
- thousandse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“drought recovery is a rapid process involving the activation of thousands of recovery-specific genes”
t0“Arabidopsis thaliana”
Claim · model-written summary
Arabidopsis leaf cell types independently developed a recovery-specific transcriptional state.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- cell type-specific transcriptional statee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identifying a cell type-specific transcriptional state developing independently across cell types”
t0“Arabidopsis thaliana”
Claim · model-written summary
Spatial transcriptomics localized recovery-induced gene expression programs across Arabidopsis leaf tissues.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- anatomical localization of recovery-induced gene expression programse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“revealing anatomical localization of recovery-induced gene expression programs across Arabidopsis leaf tissues”
Claim · model-written summary
Drought recovery autonomously activated plant immunity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- activation of the immune systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“recovery-induced activation of the immune system that occurs autonomously”
t0“Arabidopsis thaliana”
Claim · model-written summary
Drought recovery enhanced pathogen resistance in A. thaliana.
- Taxon
- A. thalianaArabidopsis thalianae0
- Treatment
- drought recoveryt0
- Outcome
- pathogen resistancee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhances pathogen resistance in vivo in A. thaliana, wild tomato (Solanum pennellii) and domesticated tomato (Solanum lycopersicum cv. M82)”
t0“Drought recovery”
Claim · model-written summary
Drought recovery enhanced pathogen resistance in wild tomato.
Not reportedComparator
Verbatim
e0“enhances pathogen resistance in vivo in A. thaliana, wild tomato (Solanum pennellii) and domesticated tomato (Solanum lycopersicum cv. M82)”
t0“Drought recovery”
Claim · model-written summary
Drought recovery enhanced pathogen resistance in domesticated tomato.
- Taxon
- domesticated tomatoe0
- Treatment
- drought recoveryt0
- Outcome
- pathogen resistancee0
- Direction
- enhancese0
Not reportedComparator
Verbatim
e0“enhances pathogen resistance in vivo in A. thaliana, wild tomato (Solanum pennellii) and domesticated tomato (Solanum lycopersicum cv. M82)”
t0“Drought recovery”
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See all 5 papers →drought · disease · Solanum lycopersicum (tomato)Positive and negative results
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See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.