Paper · 2025 · Scientific Reports

Efficient temporary pollen storage techniques for quality kiwifruit production

Sudip Kumar Dutta

11
claims
22
verbatim quotes
2
citations
3
research areas
✓
Open access · cc-by-nc-nd

Abstract

Asynchronisation in kiwifruit male and female flower anthesis is a common phenomenon across the kiwifruit-growing areas worldwide. This situation demands efficient short-term pollen storage techniques for successful kiwifruit production. Kiwifruit pollen was stored at room temperature, 0 °C, - 4 °C, - 10 °C, and - 20 °C. Six distinct storage periods- 7, 14, 21, 28, 35 and 42 days were provided for pollen collected in cryo-vials under various storage temperatures. As tested by in vitro germination and acetocarmine tests, the analysis of variance clearly showed that storage method, days of storage, and the interaction between storage methods and days of storage had a highly significant effect (p ≤ 0.0001) on pollen viability. Room-temperature storage revealed a sharp decline in pollen viability as assessed by acetocarmine test and in vitro germination test and complete viability was lost within 42 days after pollen storage. Pollen storage at 0 °C, - 4 °C, - 10 °C and - 20 °C storage revealed considerable good pollen viability in terms of in vitro germination test and acetocarmine test as compared to room-temperature storage after 42 days of storage. The rate of decline of pollen viability was in the order of 0 °C > - 4 °C > - 10 °C > - 20 °C. Pollination with low-temperature stored pollen also revealed significantly higher fruit yield and quality parameters in comparison to room-temperature stored pollen. Therefore, our findings point to a dependable approach for preserving kiwifruit pollen those farmers may apply to get good quality and high yielding fruits.

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Claims 11

Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Storage method, duration, and their interaction significantly affected kiwifruit pollen viability.

Taxon
KiwifruitActinidia deliciosae1
Treatment
storage methode0
Outcome
pollen viabilitye0
Value
p ≤ 0.0001e0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Actinidia deliciosa
Actinidia deliciosa · TaxonLuis Miguel Bugallo Sánchez ( Lmbuga ) Copyright: GFDL ( GNU Free Documentation License ) Publicada por/ Publish by : Luis Miguel Bugallo Sánchez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“storage method, days of storage, and the interaction between storage methods and days of storage had a highly significant effect (p ≤ 0.0001) on pollen viability”

✓ located verbatim in the source · abstract, characters 571–732 · show in text ↑
e1

“Kiwifruit pollen”

✓ located verbatim in the source · abstract, characters 238–254 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Room temperature storage caused a sharp decline in pollen viability.

Taxon
KiwifruitActinidia deliciosae1
Treatment
Room-temperature storagee0
Outcome
pollen viabilitye0
Direction
declinee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Actinidia deliciosa
Actinidia deliciosa · TaxonLuis Miguel Bugallo Sánchez ( Lmbuga ) Copyright: GFDL ( GNU Free Documentation License ) Publicada por/ Publish by : Luis Miguel Bugallo Sánchez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Room-temperature storage revealed a sharp decline in pollen viability as assessed by acetocarmine test and in vitro germination test”

✓ located verbatim in the source · abstract, characters 734–866 · show in text ↑
e1

“Kiwifruit pollen”

✓ located verbatim in the source · abstract, characters 238–254 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Kiwifruit pollen stored at room temperature lost all viability within 42 days.

Taxon
KiwifruitActinidia deliciosae2
Treatment
Room-temperature storagee1
Outcome
complete viability was loste0
Value
42 dayse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Actinidia deliciosa
Actinidia deliciosa · TaxonLuis Miguel Bugallo Sánchez ( Lmbuga ) Copyright: GFDL ( GNU Free Documentation License ) Publicada por/ Publish by : Luis Miguel Bugallo Sánchez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“complete viability was lost within 42 days after pollen storage”

✓ located verbatim in the source · abstract, characters 871–934 · show in text ↑
e1

“Room-temperature storage”

✓ located verbatim in the source · abstract, characters 734–758 · show in text ↑
e2

“Kiwifruit pollen”

✓ located verbatim in the source · abstract, characters 238–254 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Storage at 0 °C maintained better pollen viability than room temperature after 42 days.

Taxon
KiwifruitActinidia deliciosae1
Treatment
0 °Ce0
Outcome
pollen viabilitye0
Direction
goode0
Qualifier
after 42 days of storagee0
Comparator
room-temperature storagee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Actinidia deliciosa
Actinidia deliciosa · TaxonLuis Miguel Bugallo Sánchez ( Lmbuga ) Copyright: GFDL ( GNU Free Documentation License ) Publicada por/ Publish by : Luis Miguel Bugallo Sánchez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pollen storage at 0 °C, - 4 °C, - 10 °C and - 20 °C storage revealed considerable good pollen viability in terms of in vitro germination test and acetocarmine test as compared to room-temperature storage after 42 days of storage”

✓ located verbatim in the source · abstract, characters 936–1164 · show in text ↑
e1

“Kiwifruit pollen”

✓ located verbatim in the source · abstract, characters 238–254 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Storage at - 4 °C maintained better pollen viability than room temperature after 42 days.

Taxon
KiwifruitActinidia deliciosae1
Treatment
- 4 °Ce0
Outcome
pollen viabilitye0
Direction
goode0
Qualifier
after 42 days of storagee0
Comparator
room-temperature storagee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Actinidia deliciosa
Actinidia deliciosa · TaxonLuis Miguel Bugallo Sánchez ( Lmbuga ) Copyright: GFDL ( GNU Free Documentation License ) Publicada por/ Publish by : Luis Miguel Bugallo Sánchez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pollen storage at 0 °C, - 4 °C, - 10 °C and - 20 °C storage revealed considerable good pollen viability in terms of in vitro germination test and acetocarmine test as compared to room-temperature storage after 42 days of storage”

✓ located verbatim in the source · abstract, characters 936–1164 · show in text ↑
e1

“Kiwifruit pollen”

✓ located verbatim in the source · abstract, characters 238–254 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Storage at - 10 °C maintained better pollen viability than room temperature after 42 days.

Taxon
KiwifruitActinidia deliciosae1
Treatment
- 10 °Ce0
Outcome
pollen viabilitye0
Direction
goode0
Qualifier
after 42 days of storagee0
Comparator
room-temperature storagee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Actinidia deliciosa
Actinidia deliciosa · TaxonLuis Miguel Bugallo Sánchez ( Lmbuga ) Copyright: GFDL ( GNU Free Documentation License ) Publicada por/ Publish by : Luis Miguel Bugallo Sánchez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pollen storage at 0 °C, - 4 °C, - 10 °C and - 20 °C storage revealed considerable good pollen viability in terms of in vitro germination test and acetocarmine test as compared to room-temperature storage after 42 days of storage”

✓ located verbatim in the source · abstract, characters 936–1164 · show in text ↑
e1

“Kiwifruit pollen”

✓ located verbatim in the source · abstract, characters 238–254 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Storage at - 20 °C maintained better pollen viability than room temperature after 42 days.

Taxon
KiwifruitActinidia deliciosae1
Treatment
- 20 °Ce0
Outcome
pollen viabilitye0
Direction
goode0
Qualifier
after 42 days of storagee0
Comparator
room-temperature storagee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Actinidia deliciosa
Actinidia deliciosa · TaxonLuis Miguel Bugallo Sánchez ( Lmbuga ) Copyright: GFDL ( GNU Free Documentation License ) Publicada por/ Publish by : Luis Miguel Bugallo Sánchez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pollen storage at 0 °C, - 4 °C, - 10 °C and - 20 °C storage revealed considerable good pollen viability in terms of in vitro germination test and acetocarmine test as compared to room-temperature storage after 42 days of storage”

✓ located verbatim in the source · abstract, characters 936–1164 · show in text ↑
e1

“Kiwifruit pollen”

✓ located verbatim in the source · abstract, characters 238–254 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Viability decline was greatest at 0 °C and least at - 20 °C among cold treatments.

Taxon
KiwifruitActinidia deliciosae1
Outcome
rate of decline of pollen viabilitye0
Qualifier
0 °C > - 4 °C > - 10 °C > - 20 °Ce0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Actinidia deliciosa
Actinidia deliciosa · TaxonLuis Miguel Bugallo Sánchez ( Lmbuga ) Copyright: GFDL ( GNU Free Documentation License ) Publicada por/ Publish by : Luis Miguel Bugallo Sánchez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“rate of decline of pollen viability was in the order of 0 °C > - 4 °C > - 10 °C > - 20 °C”

✓ located verbatim in the source · abstract, characters 1170–1259 · show in text ↑
e1

“Kiwifruit pollen”

✓ located verbatim in the source · abstract, characters 238–254 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Pollination with cold-stored pollen increased fruit yield versus room temperature stored pollen.

Taxon
kiwifruitActinidia deliciosae1
Treatment
low-temperature stored pollene0
Outcome
fruit yielde0
Direction
highere0
Comparator
room-temperature stored pollene0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Actinidia deliciosa
Actinidia deliciosa · TaxonLuis Miguel Bugallo Sánchez ( Lmbuga ) Copyright: GFDL ( GNU Free Documentation License ) Publicada por/ Publish by : Luis Miguel Bugallo Sánchez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pollination with low-temperature stored pollen also revealed significantly higher fruit yield and quality parameters in comparison to room-temperature stored pollen”

✓ located verbatim in the source · abstract, characters 1261–1425 · show in text ↑
e1

“kiwifruit”

✓ located verbatim in the source · title, characters 58–67 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Pollination with cold-stored pollen improved fruit quality parameters versus room temperature stored pollen.

Taxon
kiwifruitActinidia deliciosae1
Treatment
low-temperature stored pollene0
Outcome
quality parameterse0
Direction
highere0
Comparator
room-temperature stored pollene0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Actinidia deliciosa
Actinidia deliciosa · TaxonLuis Miguel Bugallo Sánchez ( Lmbuga ) Copyright: GFDL ( GNU Free Documentation License ) Publicada por/ Publish by : Luis Miguel Bugallo Sánchez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Pollination with low-temperature stored pollen also revealed significantly higher fruit yield and quality parameters in comparison to room-temperature stored pollen”

✓ located verbatim in the source · abstract, characters 1261–1425 · show in text ↑
e1

“kiwifruit”

✓ located verbatim in the source · title, characters 58–67 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors recommend cold storage of kiwifruit pollen to obtain high-quality, high-yielding fruit.

Taxon
kiwifruitActinidia deliciosae0
Outcome
preserving kiwifruit pollene0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Actinidia deliciosa
Actinidia deliciosa · TaxonLuis Miguel Bugallo Sánchez ( Lmbuga ) Copyright: GFDL ( GNU Free Documentation License ) Publicada por/ Publish by : Luis Miguel Bugallo Sánchez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“dependable approach for preserving kiwifruit pollen those farmers may apply to get good quality and high yielding fruits”

✓ located verbatim in the source · abstract, characters 1462–1582 · show in text ↑

storage · yield · Actinidia deliciosa (kiwifruit)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

storage · quality: general · Actinidia deliciosa (kiwifruit)Positive and negative results

7 papers: 5 positive, 2 negative or no effect

4 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

3 papers more in this group

See all 7 papers →

cold · quality: general · Actinidia deliciosa (kiwifruit)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.