Abstract
Chitin triggers localised and systemic plant immune responses, making it a promising treatment for sustainable disease resistance. However, the precise molecular mechanisms underlying chitin-induced systemic effects in plants remain unknown. In this study, we investigated the effects of soil amendment with crab chitin flakes (hereafter chitin) on pattern-triggered immunity (PTI) and systemic disease resistance in various plant species. We found that soil amendment with chitin potentiates PTI and disease resistance against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 in lettuce, tomato and Arabidopsis as well as against the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), which causes powdery mildew in wheat. Using micrografting in Arabidopsis, we demonstrated that this systemic effect is dependent on active chitin perception in the roots. We also showed that induced systemic resistance (ISR) and pattern-recognition receptors (PRRs)/coreceptors, but not systemic acquired resistance (SAR), are involved in the systemic effects triggered by chitin soil amendment. These systemic effects correlated with the transcriptional upregulation of key PTI regulators in distal leaves upon chitin soil amendment. Notably, chitin-triggered systemic immunity was independent of microbes present in soil or chitin flakes. Together, these findings contribute to a better understanding of chitin-triggered systemic immunity, from active chitin perception in roots to the potentiation of PTI in the leaves, ultimately priming plants to mount enhanced defence responses against pathogen attacks. Our study provides valuable insights into the molecular mechanisms of chitin soil amendment and resulting induced immunity and highlights its potential use for sustainable crop protection strategies.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Chitin soil amendment potentiated pattern-triggered immunity.
Not reportedComparator
Verbatim
e0“soil amendment with chitin potentiates PTI”
t0“Plant”
Claim · model-written summary
Chitin amendment increased resistance to Pseudomonas syringae in lettuce.
- Taxon
- lettuce
Lactuca sativae0 - Treatment
- chitint0
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e0pathogen
- Outcome
- disease resistancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disease resistance against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 in lettuce, tomato and Arabidopsis”
t0“Chitin”
Claim · model-written summary
Chitin amendment increased resistance to Pseudomonas syringae in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- chitint0
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e0pathogen
- Outcome
- disease resistancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disease resistance against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 in lettuce, tomato and Arabidopsis”
t0“Chitin”
Claim · model-written summary
Chitin amendment increased resistance to Pseudomonas syringae in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- chitint0
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e0pathogen
- Outcome
- disease resistancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disease resistance against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 in lettuce, tomato and Arabidopsis”
t0“Chitin”
Claim · model-written summary
Chitin amendment increased wheat resistance to Blumeria graminis.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- Chitint0
- Interactor
- Blumeria graminis f. sp. tritici
Blumeria graminis f. sp. triticie0pathogen - Outcome
- powdery mildewe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“against the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), which causes powdery mildew in wheat”
t0“Chitin”
Claim · model-written summary
Systemic chitin response depended on active root chitin perception.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- rootse0
- Outcome
- systemic effecte0
- Qualifier
- active chitin perceptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using micrografting in Arabidopsis, we demonstrated that this systemic effect is dependent on active chitin perception in the roots”
Claim · model-written summary
Induced systemic resistance and pattern-recognition receptors/coreceptors participated in the systemic chitin effect.
Verbatim
e0“induced systemic resistance (ISR) and pattern-recognition receptors (PRRs)/coreceptors, but not systemic acquired resistance (SAR), are involved in the systemic effects triggered by chitin soil amendment”
t0“Plant”
Claim · model-written summary
Systemic acquired resistance was not involved in the chitin-triggered systemic effect.
Verbatim
e0“but not systemic acquired resistance (SAR), are involved in the systemic effects triggered by chitin soil amendment”
t0“Plant”
Claim · model-written summary
Chitin amendment was associated with transcriptional upregulation of key PTI regulators in distal leaves.
Verbatim
e0“systemic effects correlated with the transcriptional upregulation of key PTI regulators in distal leaves upon chitin soil amendment”
t0“Plant”
Claim · model-written summary
Chitin-triggered systemic immunity did not depend on microbes in soil or chitin flakes.
Verbatim
e0“chitin-triggered systemic immunity was independent of microbes present in soil or chitin flakes”
t0“Plant”
The same questions in other papers
resistance inducer · Pseudomonas syringae pv. tomato str. DC3000 · Lactuca sativa (garden lettuce)Results agree
1 paper in the same direction as this paper
- positive
resistance inducer · Pseudomonas syringae pv. tomato str. DC3000 · Solanum lycopersicum (tomato)Results agree
1 paper in the same direction as this paper
- positive
resistance inducer · Blumeria graminis f. sp. tritici · Triticum aestivum (wheat)Results agree
1 paper in the same direction as this paper
- positive
resistance inducer · Pseudomonas syringae pv. tomato str. DC3000 · ArabidopsisResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.