Paper · 2025 · AlQalam Journal of Medical and Applied Sciences

A study of the effect of soaking faba bean seeds in several treatments on germination, some morphological and physiological growth characteristics, and their tolerance to salt stress.

Suad Mohammed Al-Siriti; Aya Ali Alsbani; Naima Mohammed

15
claims
29
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

This study evaluated the effect of soaking broad bean Vicia faba L. seeds in various treatment solutions (calcium chloride (CaCl₂) at 1% and 0.5% concentrations, gibberellic acid (GA) at 1000 and 500 mg/L, salicylic acid (SA) at 0.5% and 0.25%, and water soaking) for 24 hours, compared to a control group (no soaking). The goal was to mitigate the negative impact of irrigation water salinity on seed germination and certain morphological and physiological growth traits of broad beans. The results showed that seeds soaked in 1% and 0.5% calcium chloride solutions achieved a 100% germination rate. Calcium chloride soaking enhanced the seeds' tolerance to salt stress by improving vital metabolic activities, which stabilized cell membranes. Post germination, these seeds also exhibited resistance to the adverse effects of salinity (induced by 0.1% sodium chloride irrigation) across all studied traits. For instance, the highest shoot lengths (53 cm with freshwater irrigation and 48.7 cm with 0.1% NaCl irrigation) were recorded in seeds soaked in 1% CaCl₂. Additionally, significant increases were observed in total plant height, internode distance, fresh shoot weight, and stem thickness compared to other treatments, and leaf area compared to the control. These improvements ultimately enhanced chlorophyll (a) and (b) levels, boosting photosynthetic efficiency. Although irrigation with 0.1% NaCl reduced these values compared to freshwater irrigation, indicating that salt stress hindered cell expansion, seed soaking in optimal CaCl₂ concentrations alleviated this effect. This supports calcium's role in enhancing plant salt tolerance by strengthening cell membranes and wall. In contrast, seeds treated with gibberellic acid and salicylic acid showed very poor germination, likely due to the excessive concentrations used, which negatively impacted seed viability.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 15

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking seeds in 1% calcium chloride produced 100% germination.

Taxon
faba beanVicia fabat0
Treatment
1%e0
Outcome
germination ratee0
Value
100%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“seeds soaked in 1% and 0.5% calcium chloride solutions achieved a 100% germination rate”

✓ located verbatim in the source · abstract, characters 512–599 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking seeds in 0.5% calcium chloride produced 100% germination.

Taxon
faba beanVicia fabat0
Treatment
0.5% calcium chloride solutionse0
Outcome
germination ratee0
Value
100%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“seeds soaked in 1% and 0.5% calcium chloride solutions achieved a 100% germination rate”

✓ located verbatim in the source · abstract, characters 512–599 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Calcium chloride soaking enhanced salt tolerance through metabolic activity and membrane stability.

Treatment
Calcium chloride soakinge0
Outcome
seeds' tolerance to salt stresse0
Direction
enhancede0
Qualifier
improving vital metabolic activities, which stabilized cell membranese0

Not reportedTaxon

Verbatim

e0

“Calcium chloride soaking enhanced the seeds' tolerance to salt stress by improving vital metabolic activities, which stabilized cell membranes”

✓ located verbatim in the source · abstract, characters 601–743 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Calcium chloride soaked seeds resisted adverse salinity effects across studied traits.

Taxon
faba beanVicia fabat0
Treatment
0.1% sodium chloride irrigatione0
Outcome
resistance to the adverse effects of salinitye0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Post germination, these seeds also exhibited resistance to the adverse effects of salinity (induced by 0.1% sodium chloride irrigation) across all studied traits”

✓ located verbatim in the source · abstract, characters 745–906 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

One percent CaCl₂ soaking produced a maximum shoot length of 53 cm under freshwater irrigation.

Taxon
faba beanVicia fabat0
Treatment
1% CaCl₂e0
Outcome
shoot lengthse0
Direction
higheste0
Value
53 cme0
Qualifier
freshwater irrigatione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the highest shoot lengths (53 cm with freshwater irrigation and 48.7 cm with 0.1% NaCl irrigation) were recorded in seeds soaked in 1% CaCl₂”

✓ located verbatim in the source · abstract, characters 922–1062 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

One percent CaCl₂ soaking produced a maximum shoot length of 48.7 cm under 0.1% NaCl irrigation.

Taxon
faba beanVicia fabat0
Treatment
1% CaCl₂e0
Outcome
shoot lengthse0
Direction
higheste0
Value
48.7 cme0
Qualifier
0.1% NaCl irrigatione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the highest shoot lengths (53 cm with freshwater irrigation and 48.7 cm with 0.1% NaCl irrigation) were recorded in seeds soaked in 1% CaCl₂”

✓ located verbatim in the source · abstract, characters 922–1062 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Soaking significantly increased plant height, internode distance, and fresh shoot weight over other treatments.

Taxon
faba beanVicia fabat0
Outcome
total plant height, internode distance, fresh shoot weighte0
Direction
increasese0
Comparator
other treatmentse0

Not reportedTreatment

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“significant increases were observed in total plant height, internode distance, fresh shoot weight, and stem thickness compared to other treatments”

✓ located verbatim in the source · abstract, characters 1078–1224 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Soaking significantly increased stem thickness over other treatments.

Taxon
faba beanVicia fabat0
Outcome
stem thicknesse0
Direction
increasese0
Comparator
other treatmentse0

Not reportedTreatment

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“significant increases were observed in total plant height, internode distance, fresh shoot weight, and stem thickness compared to other treatments”

✓ located verbatim in the source · abstract, characters 1078–1224 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking increased leaf area relative to control.

Taxon
faba beanVicia fabat0
Outcome
leaf areae0
Comparator
the controle0

Not reportedTreatmentDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“leaf area compared to the control”

✓ located verbatim in the source · abstract, characters 1230–1263 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The improvements raised chlorophyll a and b levels.

Taxon
faba beanVicia fabat0
Outcome
chlorophyll (a) and (b) levelse0
Direction
enhancede0
Qualifier
These improvementse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“These improvements ultimately enhanced chlorophyll (a) and (b) levels, boosting photosynthetic efficiency”

✓ located verbatim in the source · abstract, characters 1265–1370 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Higher chlorophyll levels boosted photosynthetic efficiency.

Taxon
faba beanVicia fabat0
Outcome
photosynthetic efficiencye0
Direction
boostinge0
Qualifier
chlorophyll (a) and (b) levelse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“enhanced chlorophyll (a) and (b) levels, boosting photosynthetic efficiency”

✓ located verbatim in the source · abstract, characters 1295–1370 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Irrigation with 0.1% NaCl reduced the studied values relative to freshwater irrigation.

Taxon
faba beanVicia fabat0
Treatment
0.1% NaCle0
Outcome
these valuese0
Direction
reducede0
Comparator
freshwater irrigatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“irrigation with 0.1% NaCl reduced these values compared to freshwater irrigation”

✓ located verbatim in the source · abstract, characters 1381–1461 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Optimal CaCl₂ soaking alleviated the salinity effect.

Taxon
faba beanVicia fabat0
Treatment
optimal CaCl₂ concentrationse0
Outcome
this effecte0
Direction
alleviatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“seed soaking in optimal CaCl₂ concentrations alleviated this effect”

✓ located verbatim in the source · abstract, characters 1516–1583 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Gibberellic acid treatment gave very poor germination.

Taxon
faba beanVicia fabat0
Treatment
gibberellic acide0
Outcome
germinatione0
Direction
very poore0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“seeds treated with gibberellic acid and salicylic acid showed very poor germination”

✓ located verbatim in the source · abstract, characters 1703–1786 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Salicylic acid treatment gave very poor germination.

Taxon
faba beanVicia fabat0
Treatment
salicylic acide0
Outcome
germinatione0
Direction
very poore0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“seeds treated with gibberellic acid and salicylic acid showed very poor germination”

✓ located verbatim in the source · abstract, characters 1703–1786 · show in text ↑
t0

“faba bean”

✓ located verbatim in the source · title, characters 33–42 · show in text ↑

salicylic acid · growth · Vicia faba (faba bean)Results differ

21 papers: 19 positive, 2 negative or no effect

1 paper in the same direction as this paper · 19 in the opposite direction

  1. positive
  2. positive
  3. positive

17 papers more in this group

See all 21 papers →

calcium chloride dihydrate · growth · Vicia faba (faba bean)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

Gibberellic acid · growth · Vicia faba (faba bean)Positive and negative results

11 papers: 10 positive, 1 negative or no effect

10 in the opposite direction

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

irrigation · disease · Vicia faba (faba bean)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.