Abstract
Faba bean (V. faba L.) cultivars Giza 5 and Giza 461 immature embryos were used to obtain callus which was used to study the effect of salinity and transformation by the Agrobacterium tumefaciens bacteria carrying the plasmid pBI121. As the salinity level increased, both fresh and dry weights of callus tissue decreased. However, NaCl + CaCl2 had a less adverse effect on both fresh and dry weights of callus tissue as compared to NaCl alone. Generally, Giza 5 cultivar produced higher fresh and dry weights compared to Giza 461 cultivar. Sugars, soluble phenols and proline concentrations increased as the salinity level increased. Proline showed higher concentration in Giza 461 cultivar compared to Giza 5 cultivar. Vicia. faba L. callus tissue proved to be sensitive to kanamycin, whereas the survival rate was only about 2% at a concentration of 75 mg/l kanamycin. Giza 5 cultivar recorded higher transformation percentage than Giza 461 cultivar. Thus, the established callus tissue transformation in the present work can offer the introduction of new desirable genes to improve V. faba L. nutritional value and increasing resistance to disease and some herbicides and consequently improving the economical plant productivity.
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Claims 8
Claim · model-written summary
Increasing salinity decreased fresh and dry weights of faba bean callus.
- Taxon
- V. faba L.
Vicia fabae1 - Treatment
- salinity levele2
- Outcome
- fresh and dry weights of callus tissuee2
- Direction
- decreasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Faba bean (V. faba L.) cultivars Giza 5 and Giza 461 immature embryos were used to obtain callus”
e2“As the salinity level increased, both fresh and dry weights of callus tissue decreased”
Claim · model-written summary
NaCl plus CaCl2 had a less adverse effect on callus fresh and dry weights than NaCl alone.
- Taxon
- V. faba L.Vicia fabae1
- Treatment
- NaCl + CaCl2e3
- Outcome
- fresh and dry weights of callus tissuee3
- Direction
- less adversee3
- Comparator
- NaCl alonee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Faba bean (V. faba L.) cultivars Giza 5 and Giza 461 immature embryos were used to obtain callus”
e3“However, NaCl + CaCl2 had a less adverse effect on both fresh and dry weights of callus tissue as compared to NaCl alone”
Claim · model-written summary
Giza 5 callus produced higher fresh and dry weights than Giza 461 callus.
- Taxon
- V. faba L.Vicia fabae1
- Material
- Giza 5e4
- Outcome
- fresh and dry weightse4
- Direction
- highere4
- Comparator
- Giza 461e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Faba bean (V. faba L.) cultivars Giza 5 and Giza 461 immature embryos were used to obtain callus”
e4“Generally, Giza 5 cultivar produced higher fresh and dry weights compared to Giza 461 cultivar”
Claim · model-written summary
Increasing salinity increased sugars, soluble phenols and proline concentrations in faba bean callus.
- Taxon
- V. faba L.Vicia fabae1
- Treatment
- salinity levele5
- Outcome
- Sugars, soluble phenols and proline concentrationse5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Faba bean (V. faba L.) cultivars Giza 5 and Giza 461 immature embryos were used to obtain callus”
e5“Sugars, soluble phenols and proline concentrations increased as the salinity level increased”
Claim · model-written summary
Giza 461 callus had higher proline concentration than Giza 5 callus.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Faba bean (V. faba L.) cultivars Giza 5 and Giza 461 immature embryos were used to obtain callus”
e6“Proline showed higher concentration in Giza 461 cultivar compared to Giza 5 cultivar”
Claim · model-written summary
Faba bean callus was sensitive to kanamycin at 75 mg/l, with about 2% survival.
- Taxon
- V. faba L.Vicia fabae1
- Treatment
- 75 mg/l kanamycine7
- Outcome
- survival ratee7
- Value
- about 2%e7
- Qualifier
- sensitivee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Faba bean (V. faba L.) cultivars Giza 5 and Giza 461 immature embryos were used to obtain callus”
e7“Vicia. faba L. callus tissue proved to be sensitive to kanamycin, whereas the survival rate was only about 2% at a concentration of 75 mg/l kanamycin”
Claim · model-written summary
Giza 5 callus had higher transformation percentage than Giza 461 callus.
- Taxon
- V. faba L.Vicia fabae1
- Material
- Giza 5e2
- Outcome
- transformation percentagee2
- Direction
- highere2
- Comparator
- Giza 461e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Faba bean (V. faba L.) cultivars Giza 5 and Giza 461 immature embryos were used to obtain callus”
e2“Giza 5 cultivar recorded higher transformation percentage than Giza 461 cultivar”
Claim · model-written summary
Callus transformation may introduce new genes to improve faba bean nutritional value.
- Taxon
- V. faba L.Vicia fabae1
- Outcome
- nutritional valuee1
- Qualifier
- introduction of new desirable genese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Thus, the established callus tissue transformation in the present work can offer the introduction of new desirable genes to improve V. faba L. nutritional value and increasing resistance to disease”
The same questions in other papers
calcium chloride dihydrate · growth · Vicia faba (faba bean)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
salinity · growth · Vicia faba (faba bean)Results differ
47 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
47 papers more in this group
See all 51 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.