Paper · 2025 · Food Science & Nutrition

Effect of Low Light Intensity With Supplemental Far‐Red Light on Growth, Yield and Quality of Broccoli Microgreens

Shirin Shahkoomahally; Irma Ortiz; Xudong Zhu; et al.

9
claims
19
verbatim quotes
6
citations
2
research areas
✓
Open access · cc-by

Abstract

ABSTRACT One of the main obstacles to indoor agriculture is the high expense of lighting energy. The purpose of this study was to investigate how to grow broccoli microgreens under low light with higher yield and better quality. Broccoli seedlings were exposed to different photosynthetic photon flux densities (PPFD) ranging from 50 to 150 μmol/m 2 s, along with supplemental far‐red (FR) light (20% of total photon flux density (TPFD)) at 50 and 75 μmol/m 2 s. Broccoli grown under 50, 75, and 100 μmol/m 2 s exhibited the highest fresh weight. As light intensity decreased, the hypocotyl length of broccoli microgreens increased. High chlorophyll, carotenoid, and anthocyanin contents were observed at 100 and 150 μmol/m 2 s, while ascorbic acid and total phenolics were higher at 50 and 75 μmol/m 2 s. The addition of FR light resulted in increased plant height, fresh weight, and antioxidant concentration. However, there was a significant decrease in total phenolic content. These results indicate that broccoli microgreen can adapt to low light with high yield and quality. Addition of FR under low light can further increase microgreen yield and plant height. Furthermore, postharvest quality and shelf‐life of microgreens under 50 and 75 μmol/m 2 s were better than those under 100 and 150 μmol/m 2 s. This research provides the platform for further managing microgreen growth under low light to reduce energy consumption for controlled environment agriculture (CEA).

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 9

Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Broccoli microgreens grown under 50, 75 and 100 μmol/m2 s had the highest fresh weight.

Treatment
50, 75, and 100 μmol/m 2 se1
Outcome
fresh weighte1
Direction
higheste1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Broccoli seedlings were exposed to different photosynthetic photon flux densities (PPFD) ranging from 50 to 150 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 229–351 · show in text ↑
e1

“Broccoli grown under 50, 75, and 100 μmol/m 2 s exhibited the highest fresh weight”

✓ located verbatim in the source · abstract, characters 463–545 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Hypocotyl length of broccoli microgreens increased as light intensity decreased.

Treatment
light intensitye2
Outcome
hypocotyl lengthe2
Direction
increasede2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Broccoli seedlings were exposed to different photosynthetic photon flux densities (PPFD) ranging from 50 to 150 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 229–351 · show in text ↑
e2

“As light intensity decreased, the hypocotyl length of broccoli microgreens increased”

✓ located verbatim in the source · abstract, characters 547–631 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Chlorophyll, carotenoid and anthocyanin contents were higher at 100 and 150 μmol/m2 s.

Treatment
100 and 150 μmol/m 2 se3
Outcome
chlorophyll, carotenoid, and anthocyanin contentse3
Direction
higherinferrede3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Broccoli seedlings were exposed to different photosynthetic photon flux densities (PPFD) ranging from 50 to 150 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 229–351 · show in text ↑
e3

“High chlorophyll, carotenoid, and anthocyanin contents were observed at 100 and 150 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 633–727 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Ascorbic acid and total phenolics were higher at 50 and 75 μmol/m2 s.

Treatment
50 and 75 μmol/m 2 se4
Outcome
ascorbic acid and total phenolicse4
Direction
highere4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Broccoli seedlings were exposed to different photosynthetic photon flux densities (PPFD) ranging from 50 to 150 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 229–351 · show in text ↑
e4

“while ascorbic acid and total phenolics were higher at 50 and 75 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 729–804 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Addition of far-red light increased plant height, fresh weight and antioxidant concentration of broccoli microgreens.

Treatment
addition of FR lighte5
Outcome
plant height, fresh weight, and antioxidant concentratione5
Direction
increasede5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Broccoli seedlings were exposed to different photosynthetic photon flux densities (PPFD) ranging from 50 to 150 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 229–351 · show in text ↑
e5

“The addition of FR light resulted in increased plant height, fresh weight, and antioxidant concentration”

✓ located verbatim in the source · abstract, characters 806–910 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Far-red light addition significantly decreased total phenolic content of broccoli microgreens.

Treatment
addition of FR lighte5
Outcome
total phenolic contente6
Direction
decreasee6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Broccoli seedlings were exposed to different photosynthetic photon flux densities (PPFD) ranging from 50 to 150 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 229–351 · show in text ↑
e5

“The addition of FR light resulted in increased plant height, fresh weight, and antioxidant concentration”

✓ located verbatim in the source · abstract, characters 806–910 · show in text ↑
e6

“However, there was a significant decrease in total phenolic content”

✓ located verbatim in the source · abstract, characters 912–979 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Postharvest quality and shelf-life of microgreens under 50 and 75 μmol/m2 s were better than under 100 and 150 μmol/m2 s.

Treatment
under 50 and 75 μmol/m 2 se7
Outcome
postharvest quality and shelf‐lifee7
Direction
bettere7
Comparator
100 and 150 μmol/m 2 se7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Broccoli seedlings were exposed to different photosynthetic photon flux densities (PPFD) ranging from 50 to 150 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 229–351 · show in text ↑
e7

“postharvest quality and shelf‐life of microgreens under 50 and 75 μmol/m 2 s were better than those under 100 and 150 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 1181–1309 · show in text ↑
Observationauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Broccoli microgreens can adapt to low light with high yield and quality.

Outcome
broccoli microgreen can adapt to low light with high yield and qualitye1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Broccoli seedlings were exposed to different photosynthetic photon flux densities (PPFD) ranging from 50 to 150 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 229–351 · show in text ↑
e1

“These results indicate that broccoli microgreen can adapt to low light with high yield and quality”

✓ located verbatim in the source · abstract, characters 981–1079 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Adding far-red light under low light can further increase microgreen yield and plant height.

Treatment
Addition of FR under low lighte1
Outcome
microgreen yield and plant heighte1
Direction
increasee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Broccoli seedlings were exposed to different photosynthetic photon flux densities (PPFD) ranging from 50 to 150 μmol/m 2 s”

✓ located verbatim in the source · abstract, characters 229–351 · show in text ↑
e1

“Addition of FR under low light can further increase microgreen yield and plant height”

✓ located verbatim in the source · abstract, characters 1081–1166 · show in text ↑

light · yield · Brassica oleracea (wild cabbage)Positive and negative results

17 papers: 14 positive, 3 negative or no effect

13 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. negative
  3. negative

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light · quality: phytochemicals · Brassica oleracea (wild cabbage)Positive and negative results

34 papers: 31 positive, 3 negative or no effect

2 papers in the same direction as this paper · 31 in the opposite direction

  1. positive
  2. positive
  3. positive

30 papers more in this group

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light · growth · Brassica oleracea (wild cabbage)Positive and negative results

54 papers: 40 positive, 14 negative or no effect

39 papers in the same direction as this paper · 13 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. positive
  3. negative

50 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.