Abstract
Glycerophosphodiester phosphodiesterase (GDPD) catalyzes the hydrolysis of glycerophosphodiesters into sn-glycerol-3-phosphate (G-3-P) and corresponding alcohols, which is integral to various physiological processes in plants. However, our comprehension of the GDPD gene family in foxtail millet ( Setaria italica L.) remains limited and unclear. This study aimed to identify and predict the function of GDPD gene family members in foxtail millet through a comprehensive genome-wide analysis.14 SiGDPD genes were identified in the foxtail millet genome. Phylogenetic analysis categorized SiGDPD proteins into five groups. Promoter regions of SiGDPD genes contained multiple cis -acting elements related to light response, hormone regulation, and stress response. Phylogenetic and collinearity analyses demonstrated conservation of GDPD proteins among foxtail millet, sorghum, rice, and maize, with the SiGDPD gene family undergoing purifying selection during evolution.Tissue differential expression analysis revealed distinct expression patterns of SiGDPD genes across various tissues, showing spatiotemporal expression characteristics. Under low phosphorus stress, the expression levels of SiGDPD3 and SiGDPD14 significantly increased, while SiGDPD1 , SiGDPD5 , SiGDPD6 , and SiGDPD11 showed significant decreases.To identify the function of SiGDPD14 , an over-expressed transgenic Arabidopsis was generated. The results showed that transgenic Arabidopsis thaliana plants over-expressing SiGDPD14 exhibited enhanced tolerance to low phosphorus stress.Taken together, the results of this study provide valuable information for further studies on candidate SiGDPD genes involved in the phosphate deficiency response in foxtail millet.
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Claims 9
Claim · model-written summary
Fourteen SiGDPD genes were identified in the foxtail millet genome.
- Taxon
- foxtail millet
Setaria italicae0 - Gene
- SiGDPDe0
- Outcome
- SiGDPD genese0
- Value
- 14e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“14 SiGDPD genes were identified in the foxtail millet genome.”
Claim · model-written summary
SiGDPD proteins fell into five phylogenetic groups.
- Taxon
- foxtail milletSetaria italicat0
- Gene
- SiGDPDe0
- Outcome
- SiGDPD proteinse0
- Value
- five groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis categorized SiGDPD proteins into five groups.”
t0“foxtail millet”
Claim · model-written summary
SiGDPD promoters contained elements associated with light, hormone and stress response.
- Taxon
- foxtail milletSetaria italicat0
- Gene
- SiGDPD genese0
- Outcome
- multiple cis -acting elements related to light response, hormone regulation, and stress responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoter regions of SiGDPD genes contained multiple cis -acting elements related to light response, hormone regulation, and stress response.”
t0“foxtail millet”
Claim · model-written summary
GDPD proteins were conserved among foxtail millet, sorghum, rice and maize.
- Taxon
- foxtail milletSetaria italicae0
- Gene
- GDPD proteinse0
- Outcome
- conservation of GDPD proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic and collinearity analyses demonstrated conservation of GDPD proteins among foxtail millet, sorghum, rice, and maize”
Claim · model-written summary
The SiGDPD family showed evidence of purifying selection.
- Taxon
- foxtail milletSetaria italicat0
- Gene
- SiGDPD gene familye0
- Outcome
- purifying selectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the SiGDPD gene family undergoing purifying selection during evolution.”
t0“foxtail millet”
Claim · model-written summary
SiGDPD genes had distinct tissue expression patterns.
- Taxon
- foxtail milletSetaria italicat0
- Organ / stage
- various tissuese0
- Gene
- SiGDPD genese0
- Outcome
- distinct expression patterns of SiGDPD genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tissue differential expression analysis revealed distinct expression patterns of SiGDPD genes across various tissues, showing spatiotemporal expression characteristics.”
t0“foxtail millet”
Claim · model-written summary
Low phosphorus significantly increased SiGDPD3 and SiGDPD14 expression.
- Taxon
- foxtail milletSetaria italicat0
- Treatment
- low phosphorus stresse0
- Gene
- SiGDPD3 and SiGDPD14e0
- Outcome
- expression levels of SiGDPD3 and SiGDPD14e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under low phosphorus stress, the expression levels of SiGDPD3 and SiGDPD14 significantly increased”
t0“foxtail millet”
Claim · model-written summary
Low phosphorus significantly decreased expression of SiGDPD1, SiGDPD5, SiGDPD6 and SiGDPD11.
- Taxon
- foxtail milletSetaria italicat0
- Treatment
- low phosphorus stresse0
- Gene
- SiGDPD1 , SiGDPD5 , SiGDPD6 , and SiGDPD11e0
- Outcome
- SiGDPD1 , SiGDPD5 , SiGDPD6 , and SiGDPD11e0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under low phosphorus stress, the expression levels of SiGDPD3 and SiGDPD14 significantly increased, while SiGDPD1 , SiGDPD5 , SiGDPD6 , and SiGDPD11 showed significant decreases.”
t0“foxtail millet”
Claim · model-written summary
Arabidopsis overexpressing SiGDPD14 had enhanced low phosphorus tolerance.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- over-expressing SiGDPD14e0
- Gene
- SiGDPD14e0
- Outcome
- tolerance to low phosphorus stresse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic Arabidopsis thaliana plants over-expressing SiGDPD14 exhibited enhanced tolerance to low phosphorus stress.”
The same questions in other papers
phosphorus · molecular: expression · Setaria italica (foxtail millet)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.