Abstract
Grapevine is naturally exposed to stresses like heat, drought, and hypoxia. A recent study found very low oxygen levels inside grape berries, linked to ethanol content. Other studies have established a link between ethanol and tolerance to various stresses: heat, drought and high salinity. The causes of such tolerances are not well understood. In our study, 3-week-old Gamay calli, Vitis vinifera, were characterized for endogenous oxygen and ethanol concentrations. A global transcriptomic study was conducted to explore the response of grapevine cells to ethanol, which, to our knowledge, is the first such analysis in plants. RNA-seq analysis was performed on cells at 6 and 24 h after treatment with 1 mM ethanol. After 6 h, ethanol addition led to 386 differentially expressed genes (DEGs), with notable upregulation of genes related to heat response, especially small Heat Shock Proteins (sHSPs). Further experiments showed that ethanol priming in grape cells or in Arabidopsis seedlings reduced pigment and electrolyte leakage under heat stress, respectively. This study supports the idea that ethanol priming helps protect plants against heat stress and provides a valuable RNA-seq dataset for further research into the underlying mechanisms, where sHSPs play a potentially crucial role in this adaptive response.
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Claims 5
Claim · model-written summary
Ethanol addition led to 386 differentially expressed genes in grapevine cells after 6 h
- Treatment
- ethanol additione1
- Outcome
- differentially expressed genes (DEGs)e1
- Value
- 386e1
- Qualifier
- After 6 he1
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Responses of grapevine cells”
e1“After 6 h, ethanol addition led to 386 differentially expressed genes (DEGs)”
Claim · model-written summary
Ethanol addition upregulated genes related to heat response in grapevine cells, especially small Heat Shock Proteins
- Treatment
- ethanol additione1
- Gene
- small Heat Shock Proteins (sHSPs)e2
- Outcome
- genes related to heat responsee2
- Direction
- upregulatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Responses of grapevine cells”
e1“After 6 h, ethanol addition led to 386 differentially expressed genes (DEGs)”
e2“with notable upregulation of genes related to heat response, especially small Heat Shock Proteins (sHSPs)”
Claim · model-written summary
Ethanol priming reduced pigment leakage in grape cells under heat stress
- Organ / stage
- grape cellse3
- Treatment
- ethanol priminge3
- Outcome
- pigmente3
- Direction
- reducede3
- Qualifier
- under heat stresse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Responses of grapevine cells”
e3“Further experiments showed that ethanol priming in grape cells or in Arabidopsis seedlings reduced pigment and electrolyte leakage under heat stress, respectively”
Claim · model-written summary
Ethanol priming reduced electrolyte leakage in Arabidopsis seedlings under heat stress
- Taxon
- Arabidopsis
Arabidopsise3 - Organ / stage
- seedlingse3
- Treatment
- ethanol priminge3
- Outcome
- electrolyte leakagee3
- Direction
- reducede3
- Qualifier
- under heat stresse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Responses of grapevine cells”
e3“Further experiments showed that ethanol priming in grape cells or in Arabidopsis seedlings reduced pigment and electrolyte leakage under heat stress, respectively”
Claim · model-written summary
Small Heat Shock Proteins may play a crucial role in the adaptive response of plants to ethanol priming
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Responses of grapevine cells”
e4“where sHSPs play a potentially crucial role in this adaptive response”
The same questions in other papers
ethanol · molecular: expression · VitisPositive and negative results
1 in the opposite direction
- no effect
seed treatment · physiology: oxidative · ArabidopsisResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.