Paper · 2025 · Tropical Journal of Phytochemistry and Pharmaceutical Sciences

Differential Phytochemical Response to Increased Nitrogen in Manihot esculenta Crantz (Cassava) Grown in Mining Soil

Imade M Eseigbe; Usunobun Usunomena

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claims
19
verbatim quotes
0
citations
2
research areas
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Open access · cc-by-nc

Abstract

Ikpeshi and Okpella, communities in Edo State, Nigeria, are areas with active mining operations. These activities can significantly impact the surrounding environment, including the plant life and, consequently, the phytochemical composition of plants growing in these areas. This study investigated the effects of nitrogen amendment on the phytochemical composition of cassava grown in mining soil. The experiment was conducted on loamy sandy soil collected from two different sites each from Ikpeshi and Okpella, and the experiment was arranged in a completely randomized design in triplicate with two treatments of 2 g and 2.5 g nitrogen. Steroids, terpenoids, flavonoids, anthraquinones, phlobatannins, saponins, cardiac glycosides with a steroidal nucleus (CG1), cardiac glycosides with deoxy sugar, cardiac glycosides with cardenolides, and phenols, except for anthraquinone, which was absent, were analyzed in the methanolic extract cassava. The highest concentration of all parameters was recorded for CG1 at Okpella site A (4.91 ± 0.16), and phlobatannin was observed to have the lowest concentration of all parameters, at 0.04 ± 0.02 for Ikpeshi site A at 2 g nitrogen. Increased nitrogen concentration significantly increased the concentrations of alkaloids, flavonoids, and phenols, while other phytochemicals remained relatively stable. At 2.5 g nitrogen, the highest flavonoid concentration was recorded for IKA (1.69 ± 0.02), the highest phenol concentration was recorded for IKB (1.39 ± 0.06), and the highest alkaloid concentration was recorded for OKB (1.69 ± 0.28). This study provides evidence that nitrogen amendment influences the phytochemical composition of cassava grown in post-mining soils from Ikpeshi and Okpella.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

Anthraquinone was absent from cassava extract.

Outcome
anthraquinone, which was absente0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“Steroids, terpenoids, flavonoids, anthraquinones, phlobatannins, saponins, cardiac glycosides with a steroidal nucleus (CG1), cardiac glycosides with deoxy sugar, cardiac glycosides with cardenolides, and phenols, except for anthraquinone, which was absent, were analyzed in the methanolic extract cassava.”

✓ located verbatim in the source · abstract, characters 642–948 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

CG1 had the highest concentration of all measured parameters at Okpella site A.

Outcome
highest concentration of all parameters was recorded for CG1e0
Direction
higheste0
Value
4.91 ± 0.16e0
Setting
Okpella site Ae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“Cassava”

✓ located verbatim in the source · title, characters 87–94 · show in text ↑
e0

“The highest concentration of all parameters was recorded for CG1 at Okpella site A (4.91 ± 0.16)”

✓ located verbatim in the source · abstract, characters 949–1045 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Phlobatannin had the lowest measured concentration at Ikpeshi site A with 2 g nitrogen.

Treatment
2 g nitrogene0
Outcome
phlobatannin was observed to have the lowest concentratione0
Direction
loweste0
Value
0.04 ± 0.02e0
Setting
Ikpeshi site Ae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“Cassava”

✓ located verbatim in the source · title, characters 87–94 · show in text ↑
e0

“phlobatannin was observed to have the lowest concentration of all parameters, at 0.04 ± 0.02 for Ikpeshi site A at 2 g nitrogen.”

✓ located verbatim in the source · abstract, characters 1051–1179 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Higher nitrogen significantly increased alkaloid concentration.

Treatment
Increased nitrogen concentratione0
Outcome
concentrations of alkaloidse0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“Cassava”

✓ located verbatim in the source · title, characters 87–94 · show in text ↑
e0

“Increased nitrogen concentration significantly increased the concentrations of alkaloids, flavonoids, and phenols”

✓ located verbatim in the source · abstract, characters 1180–1293 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Higher nitrogen significantly increased flavonoid concentration.

Treatment
Increased nitrogen concentratione0
Outcome
flavonoidse0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“Cassava”

✓ located verbatim in the source · title, characters 87–94 · show in text ↑
e0

“Increased nitrogen concentration significantly increased the concentrations of alkaloids, flavonoids, and phenols”

✓ located verbatim in the source · abstract, characters 1180–1293 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Higher nitrogen significantly increased phenol concentration.

Treatment
Increased nitrogen concentratione0
Outcome
phenolse0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“Cassava”

✓ located verbatim in the source · title, characters 87–94 · show in text ↑
e0

“Increased nitrogen concentration significantly increased the concentrations of alkaloids, flavonoids, and phenols”

✓ located verbatim in the source · abstract, characters 1180–1293 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Other measured phytochemicals remained relatively stable with increased nitrogen.

Treatment
Increased nitrogen concentratione0
Outcome
other phytochemicalse0
Direction
remained relatively stablee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“Cassava”

✓ located verbatim in the source · title, characters 87–94 · show in text ↑
e0

“Increased nitrogen concentration significantly increased the concentrations of alkaloids, flavonoids, and phenols, while other phytochemicals remained relatively stable.”

✓ located verbatim in the source · abstract, characters 1180–1349 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

At 2.5 g nitrogen, IKA had the highest flavonoid concentration of 1.69 ± 0.02.

Treatment
2.5 g nitrogene0
Outcome
flavonoid concentratione0
Direction
higheste0
Value
1.69 ± 0.02e0
Setting
IKAe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“Cassava”

✓ located verbatim in the source · title, characters 87–94 · show in text ↑
e0

“At 2.5 g nitrogen, the highest flavonoid concentration was recorded for IKA (1.69 ± 0.02)”

✓ located verbatim in the source · abstract, characters 1350–1439 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

At 2.5 g nitrogen, IKB had the highest phenol concentration of 1.39 ± 0.06.

Outcome
phenol concentratione0
Direction
higheste0
Value
1.39 ± 0.06e0
Setting
IKBe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“Cassava”

✓ located verbatim in the source · title, characters 87–94 · show in text ↑
e0

“the highest phenol concentration was recorded for IKB (1.39 ± 0.06)”

✓ located verbatim in the source · abstract, characters 1441–1508 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

At 2.5 g nitrogen, OKB had the highest alkaloid concentration of 1.69 ± 0.28.

Outcome
alkaloid concentratione0
Direction
higheste0
Value
1.69 ± 0.28e0
Setting
OKBe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Manihot esculenta
Manihot esculenta · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

t0

“Cassava”

✓ located verbatim in the source · title, characters 87–94 · show in text ↑
e0

“the highest alkaloid concentration was recorded for OKB (1.69 ± 0.28)”

✓ located verbatim in the source · abstract, characters 1514–1583 · show in text ↑

nitrogen · quality: phytochemicals · Manihot esculenta (cassava)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.