Paper · 2025 · EUREKA Life Sciences

Extraction and characterization of chitosan from the fish scale (Labeo Rohita) application on preserving nutritional quality, shelf life and sensory quality of apple samples (Malus Domestica)

Ashmita Timilsina; Bishal Thagunna; Simran Bhattarai; et al.

9
claims
12
verbatim quotes
1
citations
3
research areas
✓
Open access · cc-by

Abstract

Chitosan is a unique natural alkaline polysaccharide known for its excellent biological compatibility and biodegradability. This study emphasizes the value of post-harvest treatments for strawberries using chitosan as a method of preservation. Chitosan was extracted from fish scales (Labeo rohita) through a chemical process that involved both demineralization and deproteinization, resulting in a 1.5 % solution in its natural state. Various assessments, including physiochemical (weight loss, pH, total soluble solid, acidity, vitamin C, reducing sugar,) microbial (mesophilic bacteria, yeast and mold), sensory, and data analyses, were performed on chitosan-treated apple samples over 25 days, with comparisons made against uncoated apple samples. The total yield of chitosan from fish scale was 13.33 %. The moisture, ash and pH of the extracted chitosan was found to be 2.3 %, 2.0 % and 7.0. The chitosan was then coated to apple samples, and the sample’s physical and chemical composition was retained when compared to the uncoated samples. After the 25th day of storage, the weight loss of the coated and uncoated samples was 7.98 % and 11.86 %, from 0.87 %. Microbial growth was also noticeably lower in the treated apples compared to the control samples. After the 25th day of storage, the mesophilic bacteria in coated and uncoated samples were 3 CFU/g and 149 CFU/g. Similarly, after the 25th day of storage, the yeast and mold in coated and uncoated samples was 3 CFU/g and 170 CFU/g respectively. In terms of sensory attributes, the coated apples did not deliver any distinct flavors or aromas, and they were accepted for storage up to 25 days, while uncoated apples were not accepted before 10 days. Chitosan-coated apples received a strong level of acceptance. Therefore, chitosan extracts can serve as effective natural additives for extending the shelf-life of apples

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Claims 9

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chitosan coating retained the physical and chemical composition of apple samples relative to uncoated samples.

Treatment
chitosane0
Outcome
physical and chemical compositione0
Direction
retainede0
Comparator
uncoated samplese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“The chitosan was then coated to apple samples, and the sample’s physical and chemical composition was retained when compared to the uncoated samples.”

✓ located verbatim in the source · abstract, characters 898–1047 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

After 25 days, coated apple samples lost 7.98% weight versus 11.86% for uncoated samples.

Treatment
coatede0
Outcome
weight losse0
Direction
lowerinferrede0
Value
7.98 %e0
Qualifier
11.86 %e0
Comparator
uncoated samplese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“After the 25th day of storage, the weight loss of the coated and uncoated samples was 7.98 % and 11.86 %, from 0.87 %.”

✓ located verbatim in the source · abstract, characters 1048–1166 · show in text ↑
t0

“apple samples (Malus Domestica)”

✓ located verbatim in the source · title, characters 160–191 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Microbial growth was lower on chitosan treated apples than on controls.

Treatment
treated applese0
Outcome
Microbial growthe0
Direction
lowere0
Comparator
control samplese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Microbial growth was also noticeably lower in the treated apples compared to the control samples.”

✓ located verbatim in the source · abstract, characters 1167–1264 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 25 days, coated apples had 3 CFU/g mesophilic bacteria versus 149 CFU/g in uncoated apples.

Treatment
coatede0
Outcome
mesophilic bacteriae0
Direction
lowerinferrede0
Value
3 CFU/ge0
Qualifier
149 CFU/ge0
Comparator
uncoated samplese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“After the 25th day of storage, the mesophilic bacteria in coated and uncoated samples were 3 CFU/g and 149 CFU/g.”

✓ located verbatim in the source · abstract, characters 1265–1378 · show in text ↑
t0

“apple samples (Malus Domestica)”

✓ located verbatim in the source · title, characters 160–191 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 25 days, coated apples had 3 CFU/g yeast and mold versus 170 CFU/g in uncoated apples.

Treatment
coatede0
Outcome
yeast and molde0
Direction
lowerinferrede0
Value
3 CFU/ge0
Qualifier
170 CFU/ge0
Comparator
uncoated samplese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“after the 25th day of storage, the yeast and mold in coated and uncoated samples was 3 CFU/g and 170 CFU/g respectively.”

✓ located verbatim in the source · abstract, characters 1390–1510 · show in text ↑
t0

“apple samples (Malus Domestica)”

✓ located verbatim in the source · title, characters 160–191 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Coated apples did not develop distinct flavors or aromas.

Treatment
coatede0
Outcome
did not deliver any distinct flavors or aromase0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“the coated apples did not deliver any distinct flavors or aromas”

✓ located verbatim in the source · abstract, characters 1543–1607 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Coated apples were accepted for storage up to 25 days, whereas uncoated apples were rejected before 10 days.

Treatment
coatede0
Outcome
accepted for storagee0
Direction
up toe0
Value
25 dayse0
Qualifier
before 10 dayse0
Comparator
uncoated applese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“they were accepted for storage up to 25 days, while uncoated apples were not accepted before 10 days.”

✓ located verbatim in the source · abstract, characters 1613–1714 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Chitosan coated apples received strong acceptance.

Treatment
Chitosan-coatede0
Outcome
strong level of acceptancee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Chitosan-coated apples received a strong level of acceptance.”

✓ located verbatim in the source · abstract, characters 1715–1776 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors propose chitosan extracts as additives to extend apple shelf life.

Treatment
chitosan extractse0
Outcome
extending the shelf-life of applese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“chitosan extracts can serve as effective natural additives for extending the shelf-life of apples”

✓ located verbatim in the source · abstract, characters 1788–1885 · show in text ↑

chitosan · quality: general · Malus domestica (apple)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 reporting no difference from an alternative

  1. no effect
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.