Paper · 2025 · Fayoum Journal of Agricultural Research and Development /Fayoum Journal of Agricultural Research and Development

Soaking seeds in biostimulants restores germination, growth, and physiology of Glycine max seedlings affected by salt stress

Nada M. Nady; Mostafa M. Rady

6
claims
12
verbatim quotes
0
citations
1
research areas
✓
Open access · cc-by

Abstract

The main approach to control stressed crops is using plant biostimulants, such as diluted lemon fruit juice (DLFJ) and bee honey (DBH), which promote plant growth and physiology. This study investigated the potential promotive effects of soaking Glycine max seeds in DLFJ or DBH under normal conditions (NCs) or salt stress conditions (SSCs) on seed germination percentage (GP), seedling growth, and physiology. The result indicated that the ideal requirement for seed soaking was to soak the seeds for 8 h in 4% DLFJ or 6% DBH to obtain optimal results, with 6% DBH outperforming 4% DLFJ. The SSCs (irrigation with saline water; EC = 8.60 dS m–1) markedly reduced seed PG, seedling fresh and dry weights, root activity, photosynthetic efficiency (total chlorophyll content, PSII Fv/Fm, PSII PIABS), relative water content, and membrane stability index compared with the NCs (irrigation with normal water; EC = 1.60 dS m–1). When soybean seedlings were irrigated with normal water or saline water, soaking seeds in 4% DLFJ or 6% DBH significantly increased the above parameters compared to controls (soaking seeds in distilled water). The responses of the above parameters were generally more pronounced under SSCs than under NCs and were typically more pronounced with 6% DBH treatment than with 4% DLFJ treatment. In conclusion, 6% DBH is a natural multi-biostimulator that is significantly better than DLFJ in reducing the effects of salt stress on G. max seedlings.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Six percent honey outperformed four percent lemon juice for the tested seedling outcomes.

Taxon
Glycine max seedlingsGlycine maxt0
Treatment
6% DBHe0
Outcome
optimal resultse0
Direction
outperforminge0
Qualifier
8 he0
Comparator
4% DLFJe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Glycine max seedlings”

✓ located verbatim in the source · title, characters 79–100 · show in text ↑
e0

“soak the seeds for 8 h in 4% DLFJ or 6% DBH to obtain optimal results, with 6% DBH outperforming 4% DLFJ”

✓ located verbatim in the source · abstract, characters 484–588 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Saline irrigation reduced germination, seedling weights and root activity relative to normal irrigation.

Taxon
Glycine max seedlingsGlycine maxt0
Treatment
irrigation with saline watere0
Outcome
seed PG, seedling fresh and dry weights, root activitye0
Direction
reducede0
Comparator
irrigation with normal watere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Glycine max seedlings”

✓ located verbatim in the source · title, characters 79–100 · show in text ↑
e0

“The SSCs (irrigation with saline water; EC = 8.60 dS m–1) markedly reduced seed PG, seedling fresh and dry weights, root activity, photosynthetic efficiency (total chlorophyll content, PSII Fv/Fm, PSII PIABS), relative water content, and membrane stability index compared with the NCs (irrigation with normal water; EC = 1.60 dS m–1)”

✓ located verbatim in the source · abstract, characters 590–923 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Saline irrigation reduced photosynthetic efficiency, water content and membrane stability.

Taxon
Glycine max seedlingsGlycine maxt0
Treatment
irrigation with saline watere0
Outcome
photosynthetic efficiency (total chlorophyll content, PSII Fv/Fm, PSII PIABS), relative water content, and membrane stability indexe0
Direction
reducede0
Comparator
irrigation with normal watere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Glycine max seedlings”

✓ located verbatim in the source · title, characters 79–100 · show in text ↑
e0

“The SSCs (irrigation with saline water; EC = 8.60 dS m–1) markedly reduced seed PG, seedling fresh and dry weights, root activity, photosynthetic efficiency (total chlorophyll content, PSII Fv/Fm, PSII PIABS), relative water content, and membrane stability index compared with the NCs (irrigation with normal water; EC = 1.60 dS m–1)”

✓ located verbatim in the source · abstract, characters 590–923 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Four percent diluted lemon juice increased the measured germination, growth and physiology parameters versus water soaking.

Taxon
Glycine max seedlingsGlycine maxt0
Treatment
4% DLFJe0
Outcome
above parameterse0
Direction
increasede0
Comparator
soaking seeds in distilled watere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Glycine max seedlings”

✓ located verbatim in the source · title, characters 79–100 · show in text ↑
e0

“soaking seeds in 4% DLFJ or 6% DBH significantly increased the above parameters compared to controls (soaking seeds in distilled water)”

✓ located verbatim in the source · abstract, characters 998–1133 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Six percent diluted bee honey increased the measured germination, growth and physiology parameters versus water soaking.

Taxon
Glycine max seedlingsGlycine maxt0
Treatment
6% DBHe0
Outcome
above parameterse0
Direction
increasede0
Comparator
soaking seeds in distilled watere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Glycine max seedlings”

✓ located verbatim in the source · title, characters 79–100 · show in text ↑
e0

“soaking seeds in 4% DLFJ or 6% DBH significantly increased the above parameters compared to controls (soaking seeds in distilled water)”

✓ located verbatim in the source · abstract, characters 998–1133 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Biostimulant responses were generally stronger under saline than normal irrigation.

Taxon
Glycine max seedlingsGlycine maxt0
Treatment
SSCse0
Outcome
responses of the above parameterse0
Direction
more pronouncede0
Comparator
NCse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Glycine max seedlings”

✓ located verbatim in the source · title, characters 79–100 · show in text ↑
e0

“The responses of the above parameters were generally more pronounced under SSCs than under NCs”

✓ located verbatim in the source · abstract, characters 1135–1229 · show in text ↑

salinity · physiology: photosynthesis · Glycine max (soybean)Results differ

37 papers: 8 positive, 29 negative or no effect

28 papers in the same direction as this paper · 8 in the opposite direction

  1. positive
  2. positive
  3. positive

33 papers more in this group

See all 37 papers →

irrigation · physiology: photosynthesis · Glycine max (soybean)Positive and negative results

14 papers: 11 positive, 3 negative or no effect

2 papers in the same direction as this paper · 11 in the opposite direction

  1. positive
  2. positive
  3. positive

10 papers more in this group

See all 14 papers →

irrigation · growth · Glycine max (soybean)Positive and negative results

114 papers: 79 positive, 35 negative or no effect

34 papers in the same direction as this paper · 79 in the opposite direction

  1. positive
  2. positive
  3. positive

110 papers more in this group

See all 114 papers →

salinity · growth · Glycine max (soybean)Positive and negative results

116 papers: 6 positive, 110 negative or no effect

109 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. positive
  3. positive

112 papers more in this group

See all 116 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.