Abstract
Chromatin dynamics are essential for regulating DNA processes in response to environmental stimuli. Although ISWI-family enzymes are known to remodel chromatin by sliding nucleosomes in budding yeast, their functional roles and outputs in eukaryotes remain largely unknown. In this study, we investigated chromatin accessibility in the phytopathogenic fungus Fusarium graminearum treated with and without putrescine, a compound that rapidly induces the biosynthesis of the mycotoxin deoxynivalenol (DON). Putrescine globally alters chromatin accessibility, with the ATP-dependent chromatin remodeler FgIsw1 emerging as a key regulator. Unexpectedly, deletion of FgIsw1 did not affect the transcription of DON biosynthesis genes (Tri) but significantly disrupted transfer RNA (tRNA) transcription, leading to a dramatic decline in translation of DON biosynthesis enzymes. Mechanistically, FgIsw1 maintains nucleosome phasing in tRNA chromatin regions, ensuring efficient tRNA transcription. As a result, ΔFgIsw1 was unable to produce DON and lost its virulence on the host plant. These results highlight a novel role of chromatin remodelers in regulating protein translation through the control of tRNA transcription.
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Claims 8
Claim · model-written summary
Putrescine globally changed fungal chromatin accessibility.
- Taxon
- Fusarium graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume1 - Treatment
- putrescinee1
- Outcome
- chromatin accessibilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“Putrescine globally alters chromatin accessibility, with the ATP-dependent chromatin remodeler FgIsw1 emerging as a key regulator.”
e1“the phytopathogenic fungus Fusarium graminearum treated with and without putrescine”
Claim · model-written summary
FgIsw1 emerged as a key chromatin accessibility regulator.
- Taxon
- Fusarium graminearumFusarium graminearume1
- Gene
- FgIsw1e0
- Outcome
- key regulatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Putrescine globally alters chromatin accessibility, with the ATP-dependent chromatin remodeler FgIsw1 emerging as a key regulator.”
e1“the phytopathogenic fungus Fusarium graminearum”
Claim · model-written summary
FgIsw1 deletion did not affect DON biosynthesis gene transcription.
- Taxon
- Fusarium graminearumFusarium graminearume1
- Treatment
- deletion of FgIsw1e0
- Gene
- FgIsw1e0
- Outcome
- transcription of DON biosynthesis genes (Tri)e0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“deletion of FgIsw1 did not affect the transcription of DON biosynthesis genes (Tri)”
e1“the phytopathogenic fungus Fusarium graminearum”
Claim · model-written summary
FgIsw1 deletion significantly disrupted tRNA transcription.
- Taxon
- Fusarium graminearumFusarium graminearume1
- Treatment
- deletion of FgIsw1e0
- Gene
- FgIsw1e0
- Outcome
- transfer RNA (tRNA) transcriptione0
- Direction
- disruptede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“deletion of FgIsw1 did not affect the transcription of DON biosynthesis genes (Tri) but significantly disrupted transfer RNA (tRNA) transcription”
e1“the phytopathogenic fungus Fusarium graminearum”
Claim · model-written summary
FgIsw1 deletion caused a dramatic decline in translation of DON biosynthesis enzymes.
- Taxon
- Fusarium graminearumFusarium graminearume1
- Treatment
- deletion of FgIsw1e0
- Gene
- FgIsw1e0
- Outcome
- translation of DON biosynthesis enzymese0
- Direction
- declinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“deletion of FgIsw1 did not affect the transcription of DON biosynthesis genes (Tri) but significantly disrupted transfer RNA (tRNA) transcription, leading to a dramatic decline in translation of DON biosynthesis enzymes.”
e1“the phytopathogenic fungus Fusarium graminearum”
Claim · model-written summary
FgIsw1 maintains nucleosome phasing in tRNA chromatin regions for efficient tRNA transcription.
- Taxon
- Fusarium graminearumFusarium graminearume1
- Gene
- FgIsw1e0
- Outcome
- efficient tRNA transcriptione0
- Qualifier
- nucleosome phasing in tRNA chromatin regionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FgIsw1 maintains nucleosome phasing in tRNA chromatin regions, ensuring efficient tRNA transcription.”
e1“the phytopathogenic fungus Fusarium graminearum”
Claim · model-written summary
The FgIsw1 deletion strain was unable to produce DON.
- Taxon
- Fusarium graminearumFusarium graminearume1
- Treatment
- ΔFgIsw1e0
- Outcome
- produce DONe0
- Direction
- unablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ΔFgIsw1 was unable to produce DON and lost its virulence on the host plant.”
e1“the phytopathogenic fungus Fusarium graminearum”
Claim · model-written summary
The FgIsw1 deletion strain lost virulence on the host plant.
- Taxon
- host plante0
- Treatment
- ΔFgIsw1e0
- Interactor
- ΔFgIsw1e0pathogen
- Outcome
- virulence on the host plante0
- Direction
- loste0
Not reportedComparator
Verbatim
e0“ΔFgIsw1 was unable to produce DON and lost its virulence on the host plant.”
The same questions in other papers
loss of function · disease · Fusarium graminearumPositive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.