Abstract
FgGCN5, a GCN5 homolog in Fusarium graminearum, plays a critical role in hyphal vegetative growth, asexual and sexual reproduction, deoxynivalenol (DON) biosynthesis and plant infection. For nuclear localized GCN5, four conserved sequence motifs (I–IV) are presented in the catalytic domain and a bromodomain in the carboxy-terminus. As a lysine acetyltransferase, conserved negatively charged residues are present to neutralize the protons from lysine substrates. However, the role of conserved motifs/domains and residues in FgGCN5 are unclear. Here, we generated deletion mutant strains for each the conserved motifs/domains and a glutamate residue 130 (E130) replacement mutant. Deletion of each conserved motif in the catalytic domain and replacement of E130 site resulted in manifold defects in hyphae growth, asexual and sexual development, DON biosynthesis, and plant infection. Phenotypic defects in the mutant strains were similar to deletion mutants. The deletion of the bromodomain led a significant reduction in DON production and virulence, with no effects on hyphae growth, asexual or sexual reproduction. FgGCN5 was further found to localize to the nucleus in conidia and hyphae cells. In conclusion, FgGCN5 encodes a nuclear localized acetyltransferase. The conserved motifs in the catalytic domain and E130 are essential for correct functions of the gene. The conserved bromodomain is important for DON production and pathogen virulence. This was the first report to identify the functions of conserved motifs/domains in FgGCN5, which will contribute to our understanding of the mechanism(s) by which FgGCN5 regulates F. graminearum.
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Claims 6
Claim · model-written summary
Deleting each conserved catalytic-domain motif, or replacing E130, caused defects in hyphae growth, asexual and sexual development of F. graminearum.
- Taxon
- Fusarium graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0 - Treatment
- Deletion of each conserved motif in the catalytic domaine1
- Gene
- FgGCN5e0
- Outcome
- hyphae growth, asexual and sexual developmente1
- Direction
- decreaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“FgGCN5, a GCN5 homolog in Fusarium graminearum”
e1“Deletion of each conserved motif in the catalytic domain and replacement of E130 site resulted in manifold defects in hyphae growth, asexual and sexual development, DON biosynthesis, and plant infection”
Claim · model-written summary
Deleting catalytic-domain motifs or replacing E130 caused defects in DON biosynthesis and plant infection by F. graminearum.
- Taxon
- Fusarium graminearumFusarium graminearume0
- Treatment
- Deletion of each conserved motif in the catalytic domaine1
- Gene
- FgGCN5e0
- Outcome
- DON biosynthesis, and plant infectione1
- Direction
- decreaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FgGCN5, a GCN5 homolog in Fusarium graminearum”
e1“Deletion of each conserved motif in the catalytic domain and replacement of E130 site resulted in manifold defects in hyphae growth, asexual and sexual development, DON biosynthesis, and plant infection”
Claim · model-written summary
Phenotypic defects of the mutant strains were similar to those of deletion mutants.
- Taxon
- Fusarium graminearumFusarium graminearume0
- Treatment
- mutant strainse2
- Gene
- FgGCN5e0
- Outcome
- Phenotypic defectse2
- Direction
- no_changeinferrede2
- Qualifier
- similare2
- Comparator
- deletion mutantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FgGCN5, a GCN5 homolog in Fusarium graminearum”
e2“Phenotypic defects in the mutant strains were similar to deletion mutants”
Claim · model-written summary
Bromodomain deletion significantly reduced DON production and virulence of F. graminearum.
- Taxon
- Fusarium graminearumFusarium graminearume0
- Treatment
- The deletion of the bromodomaine3
- Gene
- FgGCN5e0
- Outcome
- DON production and virulencee3
- Direction
- reductione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FgGCN5, a GCN5 homolog in Fusarium graminearum”
e3“The deletion of the bromodomain led a significant reduction in DON production and virulence, with no effects on hyphae growth, asexual or sexual reproduction”
Claim · model-written summary
Bromodomain deletion had no effect on hyphae growth, asexual or sexual reproduction of F. graminearum.
- Taxon
- Fusarium graminearumFusarium graminearume0
- Treatment
- The deletion of the bromodomaine3
- Gene
- FgGCN5e0
- Outcome
- hyphae growth, asexual or sexual reproductione3
- Direction
- no_changeinferrede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FgGCN5, a GCN5 homolog in Fusarium graminearum”
e3“The deletion of the bromodomain led a significant reduction in DON production and virulence, with no effects on hyphae growth, asexual or sexual reproduction”
Claim · model-written summary
FgGCN5 localizes to the nucleus in conidia and hyphae cells of F. graminearum.
- Taxon
- Fusarium graminearumFusarium graminearume0
- Organ / stage
- conidia and hyphae cellse4
- Gene
- FgGCN5e0
- Outcome
- localize to the nucleuse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FgGCN5, a GCN5 homolog in Fusarium graminearum”
e4“FgGCN5 was further found to localize to the nucleus in conidia and hyphae cells”
The same questions in other papers
loss of function · pest · Fusarium graminearumPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
loss of function · disease · Fusarium graminearumPositive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.