Abstract
Tuber rot caused by Fusarium cugenangense is a serious problem in the cultivation and postharvest preservation of Gastrodia. It is essential to explore effective biocontrol methods to control this disease. In this work, we investigated the biocontrol efficiency and defense responses of an endophytic Streptomyces globisporus strain (WB03) isolated from the coralloid roots of Cycas panzhihuaensis against Gastrodia tuber rot disease. S. globisporus WB03 can secrete extracellular hydrolases that produce active compounds. Treatment with WB03 damaged the cell membranes of F. cugenangense and increased the leakage of cell contents from the pathogen’s mycelia. In addition, WB03 produced phytohormones involved in root development in Arabidopsis. Spraying WB03 on Arabidopsis leaves upregulated the expression of resistance genes PR1, PR5, and PDF1.2 and downregulated the transcription factor genes WRKY38 and WRKY64. In addition, WB03 also enhanced the activity of the antioxidant enzymes superoxide dismutase and peroxidase, reduced the symptoms of rot disease, and reduced the size of the lesion in Gastrodia tubers. This study suggests that endophytic S. globisporus WB03 may be effective in controlling tuber rot disease in Gastrodia.
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Claims 9
Claim · model-written summary
WB03 secreted extracellular hydrolases producing active compounds.
- Interactor
- S. globisporus WB03Streptomyces globisporuse0biocontrol_agent
- Outcome
- secrete extracellular hydrolases that produce active compoundse0
GastrodiaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S. globisporus WB03 can secrete extracellular hydrolases that produce active compounds.”
t0“Gastrodia”
Claim · model-written summary
WB03 damaged F. cugenangense cell membranes.
- Treatment
- Treatment with WB03e0
- Interactor
- F. cugenangenseFusarium cugenangensee0pathogen
- Second interactor
- WB03e0biocontrol_agent
- Outcome
- cell membranes of F. cugenangensee0
- Direction
- damagede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with WB03 damaged the cell membranes of F. cugenangense”
t0“Gastrodia”
Claim · model-written summary
WB03 increased leakage of F. cugenangense mycelial contents.
- Treatment
- WB03e1
- Interactor
- F. cugenangenseFusarium cugenangensee2pathogene0
- Outcome
- leakage of cell contents from the pathogen’s myceliae0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased the leakage of cell contents from the pathogen’s mycelia.”
t0“Gastrodia”
e1“S. globisporus WB03 can secrete extracellular hydrolases that produce active compounds.”
e2“Treatment with WB03 damaged the cell membranes of F. cugenangense”
e3“S. globisporus WB03 can secrete extracellular hydrolases that produce active compounds.”
Claim · model-written summary
WB03 produced phytohormones involved in Arabidopsis root development.
- Taxon
- Arabidopsis
Arabidopsise0 - Interactor
- WB03e0biocontrol_agent
- Outcome
- phytohormones involved in root developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WB03 produced phytohormones involved in root development in Arabidopsis.”
Claim · model-written summary
WB03 leaf spraying upregulated PR1, PR5 and PDF1.2 in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Spraying WB03e0
- Interactor
- WB03e0biocontrol_agent
- Gene
- PR1, PR5, and PDF1.2e0
- Outcome
- expression of resistance genese0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Spraying WB03 on Arabidopsis leaves upregulated the expression of resistance genes PR1, PR5, and PDF1.2”
Claim · model-written summary
WB03 leaf spraying downregulated WRKY38 and WRKY64 in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise3
- Treatment
- Spraying WB03e1
- Gene
- WRKY38 and WRKY64e0
- Outcome
- transcription factor genes WRKY38 and WRKY64e0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“downregulated the transcription factor genes WRKY38 and WRKY64.”
t0“Gastrodia elata”
e1“Spraying WB03 on Arabidopsis leaves upregulated the expression of resistance genes PR1, PR5, and PDF1.2”
e2“S. globisporus WB03 can secrete extracellular hydrolases that produce active compounds.”
e3“Spraying WB03 on Arabidopsis leaves”
Claim · model-written summary
WB03 enhanced superoxide dismutase and peroxidase activity in Gastrodia tubers.
- Treatment
- WB03e0
- Interactor
- WB03e0biocontrol_agent
- Outcome
- activity of the antioxidant enzymes superoxide dismutase and peroxidasee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WB03 also enhanced the activity of the antioxidant enzymes superoxide dismutase and peroxidase”
t0“Gastrodia”
Claim · model-written summary
WB03 reduced rot disease symptoms in Gastrodia tubers.
- Treatment
- WB03e1
- Interactor
- Fusarium cugenangenseFusarium cugenangenset0pathogene0
- Outcome
- symptoms of rot diseasee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced the symptoms of rot disease, and reduced the size of the lesion in Gastrodia tubers.”
t0“Fusarium cugenangense Causing Gastrodia”
e1“S. globisporus WB03 can secrete extracellular hydrolases that produce active compounds.”
e2“S. globisporus WB03 can secrete extracellular hydrolases that produce active compounds.”
Claim · model-written summary
WB03 reduced lesion size in Gastrodia tubers.
- Treatment
- WB03e1
- Interactor
- Fusarium cugenangenseFusarium cugenangenset0pathogene0
- Outcome
- size of the lesione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced the size of the lesion in Gastrodia tubers.”
t0“Fusarium cugenangense Causing Gastrodia”
e1“S. globisporus WB03 can secrete extracellular hydrolases that produce active compounds.”
e2“S. globisporus WB03 can secrete extracellular hydrolases that produce active compounds.”
The same questions in other papers
application · molecular: expression · ArabidopsisResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.