Abstract
BACKGROUND: The AP2/ERF (APETALA 2/ethylene-responsive element binding factors) is a class of superfamily of plant-specific transcription factor that play an important regulatory role in many physiological and biochemical processes in plants. RESULTS: In this study, overexpression of the StERF79 gene increased drought tolerance in potato plants, whereas StERF79 RNA interference expression (RNAi) lines decreased drought tolerance in potato plants. In addition, the superoxide dismutase (SOD), peroxide dismutase (POD), and catalase (CAT) activities, as well as proline (Pro) content of StERF79 transgenic lines, showed significantly higher results than those of the wild type (WT) potato plants under natural drought stress conditions, while the malondialdehyde (MDA) content was lower. The StERF79 transcription factor can respond to drought stress by interacting with a DRE cis-acting element in the promoter region of the downstream target gene (StDHN-2), and activating its expression, the result was validated by using yeast one hybrid (Y1H), Dual-Luciferase and β-glucuronidase (GUS) staining assays both in vivo and in vitro. The StDHN-2 gene is a member of the dehydrin (DHN) subfamily of the potato plant late embryonic developmentally abundant (LEA) protein family. LEA, hydrophilic proteins found in plants, serve as cellular dewatering protectants to prevent desiccation during various stresses. CONCLUSION: The results could provide novel knowledge into the functional analysis of the StERF79 gene in positive regulation of the StDHN-2 gene to drought response and its possible mechanisms in potato plants. CLINICAL TRIAL NUMBER: Not applicable.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
StERF79 overexpression increased potato drought tolerance.
- Taxon
- potato plants
Solanum tuberosume0 - Treatment
- overexpression of the StERF79 genee0
- Gene
- StERF79e0
- Outcome
- drought tolerancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of the StERF79 gene increased drought tolerance in potato plants”
Claim · model-written summary
StERF79 RNA interference decreased potato drought tolerance.
- Taxon
- potato plantsSolanum tuberosume0
- Treatment
- StERF79 RNA interference expression (RNAi) linese0
- Gene
- StERF79e0
- Outcome
- drought tolerancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StERF79 RNA interference expression (RNAi) lines decreased drought tolerance in potato plants”
Claim · model-written summary
SOD, POD and CAT activities were significantly higher in StERF79 transgenic lines than wild type under drought.
- Taxon
- potato plantsSolanum tuberosume0
- Material
- StERF79 transgenic linese0
- Outcome
- superoxide dismutase (SOD), peroxide dismutase (POD), and catalase (CAT) activitiese0
- Direction
- significantly highere0
- Qualifier
- natural drought stress conditionse0
- Comparator
- wild type (WT) potato plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the superoxide dismutase (SOD), peroxide dismutase (POD), and catalase (CAT) activities, as well as proline (Pro) content of StERF79 transgenic lines, showed significantly higher results than those of the wild type (WT) potato plants under natural drought stress conditions”
Claim · model-written summary
Proline content was significantly higher in StERF79 transgenic lines than wild type under drought.
- Taxon
- potato plantsSolanum tuberosume0
- Material
- StERF79 transgenic linese0
- Outcome
- proline (Pro) contente0
- Direction
- significantly highere0
- Qualifier
- natural drought stress conditionse0
- Comparator
- wild type (WT) potato plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proline (Pro) content of StERF79 transgenic lines, showed significantly higher results than those of the wild type (WT) potato plants under natural drought stress conditions”
Claim · model-written summary
MDA content was lower in StERF79 transgenic lines.
- Taxon
- potato (Solanum tuberosum L.)Solanum tuberosumt0
- Outcome
- malondialdehyde (MDA) contente0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato (Solanum tuberosum L.)”
e0“while the malondialdehyde (MDA) content was lower”
Claim · model-written summary
StERF79 interacted with a DRE element in the StDHN-2 promoter and activated StDHN-2 expression under drought.
- Taxon
- potato (Solanum tuberosum L.)Solanum tuberosumt0
- Gene
- StERF79e0
- Outcome
- activating its expressione0
- Qualifier
- interacting with a DRE cis-acting element in the promoter region of the downstream target gene (StDHN-2)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potato (Solanum tuberosum L.)”
e0“The StERF79 transcription factor can respond to drought stress by interacting with a DRE cis-acting element in the promoter region of the downstream target gene (StDHN-2), and activating its expression”
Claim · model-written summary
StDHN-2 was classified within the potato LEA dehydrin subfamily.
- Taxon
- potato plantSolanum tuberosume0
- Gene
- StDHN-2e0
- Outcome
- member of the dehydrin (DHN) subfamilye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The StDHN-2 gene is a member of the dehydrin (DHN) subfamily of the potato plant late embryonic developmentally abundant (LEA) protein family”
The same questions in other papers
loss of function · tolerance: abiotic · Solanum tuberosum (potato)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.