Abstract
Crown gall disease (CGD), which is caused by the bacterium Agrobacterium, is a common plant disease that often results in significant economic losses. Biocontrol offers an environmentally friendly and sustainable method to control CGD. In this study, we investigated the biocontrol effect and mechanism of CGD in Japanese cherry trees by Bacillus velezensis FZB42, a prototype strain of the plant growth-promoting rhizobacteria (PGPR). We found that the B. velezensis FZB42 mutants unable to produce one or a combination of four antimicrobial compounds (surfactin, macrolactin, bacilysin, and difficidin) significantly reduced their inhibitory activities against A. tumefaciens XYT-58 and their ability to control the occurrence of CGD. Using the purified compounds, we further demonstrated that bacilysin and surfactin can inhibit the growth of XYT-58 in vitro. Inoculation with B. velezensis FZB42 significantly increased the expression of genes related to the jasmonic acid, ethylene, and salicylic acid pathways in cherry seedlings when they were infected with XYT-58. To our knowledge, this is the first report that bacilysin and difficidin can also suppress the growth of Agrobacterium. Furthermore, our results indicate that multiple antimicrobial compounds and induced systemic resistance are involved in the biocontrol of CGD by B. velezensis. The mechanism elucidated here provides guidance for the development and application of this type of PGPR strain in the biocontrol of CGD.
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Claims 8
Claim · model-written summary
Loss of antimicrobial compound production reduced FZB42 mutant inhibition of A. tumefaciens XYT-58.
- Taxon
- A. tumefaciens XYT-58
Agrobacterium tumefaciense0 - Treatment
- B. velezensis FZB42 mutants unable to produce one or a combination of four antimicrobial compoundse0
- Interactor
- B. velezensis FZB42
Bacillus velezensise0biocontrol_agent - Outcome
- inhibitory activities against A. tumefaciens XYT-58e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B. velezensis FZB42 mutants unable to produce one or a combination of four antimicrobial compounds (surfactin, macrolactin, bacilysin, and difficidin) significantly reduced their inhibitory activities against A. tumefaciens XYT-58”
Claim · model-written summary
Those FZB42 mutants had reduced ability to control crown gall in cherry.
- Taxon
- Japanese cherry treese1
- Treatment
- B. velezensis FZB42 mutants unable to produce one or a combination of four antimicrobial compoundse0
- Interactor
- A. tumefaciens XYT-58Agrobacterium tumefaciense0pathogen
- Second interactor
- B. velezensis FZB42Bacillus velezensise0biocontrol_agent
- Outcome
- ability to control the occurrence of CGDe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B. velezensis FZB42 mutants unable to produce one or a combination of four antimicrobial compounds (surfactin, macrolactin, bacilysin, and difficidin) significantly reduced their inhibitory activities against A. tumefaciens XYT-58 and their ability to control the occurrence of CGD.”
e1“CGD in Japanese cherry trees by Bacillus velezensis FZB42”
Claim · model-written summary
Purified bacilysin inhibited XYT-58 growth in vitro.
Not reportedComparator
Verbatim
e0“bacilysin and surfactin can inhibit the growth of XYT-58 in vitro.”
Claim · model-written summary
Purified surfactin inhibited XYT-58 growth in vitro.
Not reportedComparator
Verbatim
e0“bacilysin and surfactin can inhibit the growth of XYT-58 in vitro.”
Claim · model-written summary
FZB42 inoculation increased expression of jasmonic acid, ethylene and salicylic acid pathway genes in infected cherry seedlings.
- Taxon
- cherrye0
- Organ / stage
- seedlingse0
- Treatment
- Inoculation with B. velezensis FZB42e0
- Interactor
- XYT-58e0pathogen
- Second interactor
- B. velezensis FZB42Bacillus velezensise0biocontrol_agent
- Outcome
- expression of genes related to the jasmonic acid, ethylene, and salicylic acid pathwayse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation with B. velezensis FZB42 significantly increased the expression of genes related to the jasmonic acid, ethylene, and salicylic acid pathways in cherry seedlings when they were infected with XYT-58.”
Claim · model-written summary
Bacilysin suppressed Agrobacterium growth.
- Taxon
- Agrobacterium
.jpg?width=60)
Agrobacteriume0 - Treatment
- bacilysine0
- Outcome
- growth of Agrobacteriume0
- Direction
- suppresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“bacilysin and difficidin can also suppress the growth of Agrobacterium.”
Claim · model-written summary
Difficidin suppressed Agrobacterium growth.
- Taxon
- AgrobacteriumAgrobacteriume0
- Treatment
- difficidine0
- Outcome
- growth of Agrobacteriume0
- Direction
- suppresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bacilysin and difficidin can also suppress the growth of Agrobacterium.”
Claim · model-written summary
Multiple antimicrobial compounds and induced systemic resistance contribute to FZB42 control of crown gall.
- Taxon
- Japanese cherry treese1
- Interactor
- B. velezensisBacillus velezensise0biocontrol_agent
- Outcome
- biocontrol of CGDe0
- Qualifier
- multiple antimicrobial compounds and induced systemic resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“multiple antimicrobial compounds and induced systemic resistance are involved in the biocontrol of CGD by B. velezensis.”
e1“CGD in Japanese cherry trees by Bacillus velezensis FZB42”