Abstract
Respiratory burst oxidase homologs (Rboh) genes is essential for synthesizing reactive oxygen species, which play a crucial role in environmental stress response. The Rboh gene family has been studied in model plants such as Arabidopsis. Nevertheless, Rboh remained largely unexplored in Rice (Oryza sativa L.). Here, we performed characterization of the Rboh genes family in rice (OsRboh) under Xanthomonas oryzae pv. oryzae (Xoo), salicylic acid (SA), and methyl jasmonate (MeJA) treatments. Nine OsRboh genes were retrieved distributed across six chromosomes (1, 5, 8, 9, 11, 12).These genes vary in amino acid sequence length (728-1034), isoelectric point (9.05-9.84), and molecular weight (8.341-115.014 kDa). Analysis of gene structure, motifs and conserved domains showed that OsRboh genes have similar protein sequences and functions. The promoter region of OsRboh genes was found to contain mainly cis-acting elements associated with light, jasmonic acid (JA), abscisic acid (ABA), and SA responsiveness. Predictions of functional protein-protein interaction showed that OsRboh genes were associated with MAPK signaling, plant-pathogen interaction, and other mRNA surveillance pathways. Prediction of miRNA targets and post-translational modification sites indicated that OsRboh genes may be regulated by miRNA and protein phosphorylation. Phylogenetic analysis showed that OsRboh genes were distributed into 7 clusters. Furthermore, 9 OsRboh genes were differentially expressed in different tissues (roots, stems, and leaves). OsRbohA, OsRbohB, and OsRbohD are significant genes in rice defense responses, showing unique and increased expression profiles under (Xoo-PXO99), (MeJA), and (SA) treatments. These genes important function in triggering defense mechanisms is further stressed by the high (> 20-fold) changes in expression they exhibit under these treatments. These findings enhance our understanding of rice OsRboh genes functions and contribute to stress tolerance improvement strategies.
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Claims 9
Claim · model-written summary
Nine OsRboh genes occur across six rice chromosomes.
- Taxon
- rice
Oryza sativat0 - Gene
- OsRbohe0
- Outcome
- Nine OsRboh genese0
- Value
- Ninee0
- Qualifier
- six chromosomes (1, 5, 8, 9, 11, 12)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nine OsRboh genes were retrieved distributed across six chromosomes (1, 5, 8, 9, 11, 12).”
t0“rice”
Claim · model-written summary
OsRboh proteins vary in sequence length, isoelectric point, and molecular weight.
- Taxon
- riceOryza sativat0
- Outcome
- amino acid sequence length (728-1034), isoelectric point (9.05-9.84), and molecular weight (8.341-115.014 kDa)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These genes vary in amino acid sequence length (728-1034), isoelectric point (9.05-9.84), and molecular weight (8.341-115.014 kDa).”
t0“rice”
Claim · model-written summary
OsRboh genes have similar protein sequences and functions.
- Taxon
- riceOryza sativat0
- Gene
- OsRbohe0
- Outcome
- similar protein sequences and functionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of gene structure, motifs and conserved domains showed that OsRboh genes have similar protein sequences and functions.”
t0“rice”
Claim · model-written summary
OsRboh promoters mainly contain elements responsive to light, JA, ABA, and SA.
- Taxon
- riceOryza sativat0
- Gene
- OsRbohe0
- Outcome
- cis-acting elements associated with light, jasmonic acid (JA), abscisic acid (ABA), and SA responsivenesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The promoter region of OsRboh genes was found to contain mainly cis-acting elements associated with light, jasmonic acid (JA), abscisic acid (ABA), and SA responsiveness.”
t0“rice”
Claim · model-written summary
Predicted OsRboh interactions involve MAPK signaling, plant-pathogen interaction, and mRNA surveillance.
- Taxon
- riceOryza sativat0
- Gene
- OsRbohe0
- Outcome
- MAPK signaling, plant-pathogen interaction, and other mRNA surveillance pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Predictions of functional protein-protein interaction showed that OsRboh genes were associated with MAPK signaling, plant-pathogen interaction, and other mRNA surveillance pathways.”
t0“rice”
Claim · model-written summary
Predicted miRNA targets and modification sites suggest regulation of OsRboh genes by miRNA and phosphorylation.
- Taxon
- riceOryza sativat0
- Gene
- OsRbohe0
- Outcome
- regulated by miRNA and protein phosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Prediction of miRNA targets and post-translational modification sites indicated that OsRboh genes may be regulated by miRNA and protein phosphorylation.”
t0“rice”
Claim · model-written summary
OsRboh genes formed seven phylogenetic clusters.
- Taxon
- riceOryza sativat0
- Gene
- OsRbohe0
- Outcome
- distributed into 7 clusterse0
- Value
- 7 clusterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis showed that OsRboh genes were distributed into 7 clusters.”
t0“rice”
Claim · model-written summary
Nine OsRboh genes differed in expression among roots, stems, and leaves.
- Taxon
- riceOryza sativat0
- Organ / stage
- roots, stems, and leavese0
- Gene
- OsRbohe0
- Outcome
- differentially expressede0
- Value
- 9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, 9 OsRboh genes were differentially expressed in different tissues (roots, stems, and leaves).”
t0“rice”
Claim · model-written summary
OsRbohA, OsRbohB, and OsRbohD showed increased expression under Xoo-PXO99, MeJA, and SA treatments, with changes exceeding 20-fold.
- Taxon
- riceOryza sativae0
- Treatment
- (Xoo-PXO99), (MeJA), and (SA) treatmentse0
- Interactor
- Xoo-PXO99e0pathogen
- Gene
- OsRbohA, OsRbohB, and OsRbohDe0
- Outcome
- expression profilese0
- Direction
- increasede0
- Value
- > 20-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsRbohA, OsRbohB, and OsRbohD are significant genes in rice defense responses, showing unique and increased expression profiles under (Xoo-PXO99), (MeJA), and (SA) treatments. These genes important function in triggering defense mechanisms is further stressed by the high (> 20-fold) changes in expression they exhibit under these treatments.”
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See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.