Abstract
This survey aims to develop a successful protocol through in vitro multiplication and ex vitro rooting of a native shrub, i.e., Myrtus communis L., in order to obtain the rapid production of large number of healthy plants. The in vitro proliferation showed how the two different genotypes (MR and MB) produced higher values in terms of shoot lengths, mean multiplication index (MMI) and number of nodes when treated with 6-benzylaminopurine (BAP) compared to the thidiazuron (TDZ) + 1-naphthalene acetic acid (NAA) combination. Concerning in vitro rooting, the experiment resulted in a high rooting rate (75%) and an average number of roots (5.13) by quickly dipping the basal portions in an indole-3-butyric acid (IBA) concentration of 3300 mg L−1, followed by transfer to a hormone-free growth medium (BM). In comparison, the other two concentrations tested (0.3 and 0.6 mg L−1) resulted in higher root length values. During the acclimatization phase, the study showed how the different treatments affected the development of the test shoots differently. In particular, shoots of both genotypes pre-treated by quickly dipping them in Clonex® to induce ex vitro rooting showed abundant root production only 10 days after the treatment compared to the in vitro rooting, in which roots were formed after 30 days. This suggests that ex vitro pre-treatment with Clonex® not only accelerates the rooting process compared to in vitro treatment, resulting in lower costs and easier processing, but also significantly increases root density, contributing to improved transplant success and plantlets quality. These results support the thesis that ex vitro rooting optimises plant propagation protocols, offering economic and practical advantages for nurseries and other propagation systems.
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Claims 6
Claim · model-written summary
BAP increased shoot length, multiplication index and nodes in both genotypes relative to TDZ+NAA.
- Taxon
- Myrtus communis L.
_in_Athens_on_July_4%2C_2023.jpg?width=60)
Myrtus communist0 - Treatment
- 6-benzylaminopurine (BAP)e0
- Outcome
- shoot lengths, mean multiplication index (MMI) and number of nodese0
- Direction
- highere0
- Comparator
- thidiazuron (TDZ) + 1-naphthalene acetic acid (NAA) combinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_in_Athens_on_July_4%2C_2023.jpg?width=500)
Verbatim
e0“the two different genotypes (MR and MB) produced higher values in terms of shoot lengths, mean multiplication index (MMI) and number of nodes when treated with 6-benzylaminopurine (BAP) compared to the thidiazuron (TDZ) + 1-naphthalene acetic acid (NAA) combination”
t0“Myrtus communis L.”
Claim · model-written summary
IBA dip at 3300 mg L−1 yielded 75% rooting.
- Taxon
- Myrtus communis L.Myrtus communist0
- Treatment
- indole-3-butyric acid (IBA) concentration of 3300 mg L−1e0
- Outcome
- rooting ratee0
- Direction
- highe0
- Value
- 75%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high rooting rate (75%) and an average number of roots (5.13) by quickly dipping the basal portions in an indole-3-butyric acid (IBA) concentration of 3300 mg L−1”
t0“Myrtus communis L.”
Claim · model-written summary
IBA dip at 3300 mg L−1 yielded 5.13 roots on average.
- Taxon
- Myrtus communis L.Myrtus communist0
- Treatment
- indole-3-butyric acid (IBA) concentration of 3300 mg L−1e0
- Outcome
- average number of rootse0
- Value
- 5.13e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high rooting rate (75%) and an average number of roots (5.13) by quickly dipping the basal portions in an indole-3-butyric acid (IBA) concentration of 3300 mg L−1”
t0“Myrtus communis L.”
Claim · model-written summary
The two lower IBA concentrations produced longer roots.
- Taxon
- Myrtus communis L.Myrtus communist0
- Treatment
- 0.3 and 0.6 mg L−1e0
- Outcome
- root length valuese0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the other two concentrations tested (0.3 and 0.6 mg L−1) resulted in higher root length values”
t0“Myrtus communis L.”
Claim · model-written summary
Clonex pretreatment produced abundant roots by day 10 versus day 30 for in vitro rooting.
- Taxon
- Myrtus communis L.Myrtus communist0
- Treatment
- Clonex®e0
- Outcome
- root productione0
- Direction
- abundante0
- Value
- 10 dayse0
- Qualifier
- 30 dayse0
- Comparator
- in vitro rootinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoots of both genotypes pre-treated by quickly dipping them in Clonex® to induce ex vitro rooting showed abundant root production only 10 days after the treatment compared to the in vitro rooting, in which roots were formed after 30 days”
t0“Myrtus communis L.”
Claim · model-written summary
Authors concluded ex vitro Clonex pretreatment accelerates rooting and increases root density.
- Taxon
- Myrtus communis L.Myrtus communist0
- Treatment
- Clonex®e0
- Outcome
- root densitye0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ex vitro pre-treatment with Clonex® not only accelerates the rooting process compared to in vitro treatment, resulting in lower costs and easier processing, but also significantly increases root density”
t0“Myrtus communis L.”
The same questions in other papers
N-benzyladenine · pest · Myrtus communisResults agree
1 paper in the same direction as this paper
- positive
indole-3-butyric acid · growth · Myrtus communisResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.