Paper · 2020 · Asian Journal of Agricultural and Horticultural Research

Trials on In vitro Propagation and Using Natural Additives for Myrtus communis L. Plant

Y. A. A. Ghatas

9
claims
27
verbatim quotes
1
citations
3
research areas
✓
Open access

Abstract

Explant types, anti-oxidant pre-treatments, organic additives, natural additives, vitamins mix strengths, cytokinin types and concentrations, different medium strengths, GA3 concentrations, auxin types and concentrations were studied during the period from 2017 to 2018 to establish a protocol for in vitro propagation of Myrtle. It was found that culturing of pre-treated shoot tips with anti-oxidant solution (A.O.S) on modified Murashige and Skoog (MS), or Murashige and Skoog medium supplemented with PVP as anti-oxidants induced the best results in reducing free phenolic compounds and enhancing explant development parameters. Also, adding combination of tryptophan, adenine sulphate and coconut water as organic additives maximized survival percentage and improved explant development. In the same time, adding combination of coconut water at 5% plus Banana pulp plus Papaya extract at 50 g/L of were helpful in maximizing number of shoots/plant, shoot length and greening parameters. Also duplicating the dose of vitamin mix of Gamborg medium improved explants development and survival (%) of explant. Meanwhile, using of 1.0 mg/ L BAP increased proliferation. Meanwhile, addition of 2.0 mg/ L GA3 to half strength medium maximized shoot length. Moreover, the addition of 2.0 mg/L IBA to the culture medium induced the highest number of roots/plant.

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Claims 9

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Culturing pre-treated shoot tips with anti-oxidant solution gave the best results in reducing free phenolic compounds in vitro.

Taxon
Myrtus communis L.Myrtus communist0
Treatment
anti-oxidant solution (A.O.S)e1
Outcome
free phenolic compoundse1
Direction
reducinge1
Setting
in vitroe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Myrtus communis
Myrtus communis · TaxonGeorge E. Koronaios · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

t0

“Myrtus communis L.”

✓ located verbatim in the source · title, characters 63–81 · show in text ↑
e0

“to establish a protocol for in vitro propagation of Myrtle”

✓ located verbatim in the source · abstract, characters 270–328 · show in text ↑
e1

“culturing of pre-treated shoot tips with anti-oxidant solution (A.O.S) on modified Murashige and Skoog (MS), or Murashige and Skoog medium supplemented with PVP as anti-oxidants induced the best results in reducing free phenolic compounds and enhancing explant development parameters”

✓ located verbatim in the source · abstract, characters 348–631 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Culturing pre-treated shoot tips with anti-oxidant solution gave the best results in enhancing explant development parameters in vitro.

Taxon
Myrtus communis L.Myrtus communist0
Treatment
anti-oxidant solution (A.O.S)e1
Outcome
explant development parameterse1
Direction
enhancinge1
Setting
in vitroe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Myrtus communis
Myrtus communis · TaxonGeorge E. Koronaios · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

t0

“Myrtus communis L.”

✓ located verbatim in the source · title, characters 63–81 · show in text ↑
e0

“to establish a protocol for in vitro propagation of Myrtle”

✓ located verbatim in the source · abstract, characters 270–328 · show in text ↑
e1

“culturing of pre-treated shoot tips with anti-oxidant solution (A.O.S) on modified Murashige and Skoog (MS), or Murashige and Skoog medium supplemented with PVP as anti-oxidants induced the best results in reducing free phenolic compounds and enhancing explant development parameters”

✓ located verbatim in the source · abstract, characters 348–631 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined tryptophan, adenine sulphate and coconut water as organic additives maximized survival percentage of explants.

Taxon
Myrtus communis L.Myrtus communist0
Treatment
combination of tryptophan, adenine sulphate and coconut watere2
Outcome
survival percentagee2
Direction
maximizede2
Setting
in vitroe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Myrtus communis
Myrtus communis · TaxonGeorge E. Koronaios · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

t0

“Myrtus communis L.”

✓ located verbatim in the source · title, characters 63–81 · show in text ↑
e0

“to establish a protocol for in vitro propagation of Myrtle”

✓ located verbatim in the source · abstract, characters 270–328 · show in text ↑
e2

“adding combination of tryptophan, adenine sulphate and coconut water as organic additives maximized survival percentage and improved explant development”

✓ located verbatim in the source · abstract, characters 639–791 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined tryptophan, adenine sulphate and coconut water as organic additives improved explant development.

Taxon
Myrtus communis L.Myrtus communist0
Treatment
combination of tryptophan, adenine sulphate and coconut watere2
Outcome
explant developmente2
Direction
improvede2
Setting
in vitroe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Myrtus communis
Myrtus communis · TaxonGeorge E. Koronaios · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

t0

“Myrtus communis L.”

✓ located verbatim in the source · title, characters 63–81 · show in text ↑
e0

“to establish a protocol for in vitro propagation of Myrtle”

✓ located verbatim in the source · abstract, characters 270–328 · show in text ↑
e2

“adding combination of tryptophan, adenine sulphate and coconut water as organic additives maximized survival percentage and improved explant development”

✓ located verbatim in the source · abstract, characters 639–791 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Coconut water at 5% with banana pulp and papaya extract maximized shoot number, shoot length and greening parameters.

Taxon
Myrtus communis L.Myrtus communist0
Treatment
coconut water at 5% plus Banana pulp plus Papaya extract at 50 g/Le3
Outcome
number of shoots/plant, shoot length and greening parameterse3
Direction
maximizinge3
Setting
in vitroe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Myrtus communis
Myrtus communis · TaxonGeorge E. Koronaios · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

t0

“Myrtus communis L.”

✓ located verbatim in the source · title, characters 63–81 · show in text ↑
e0

“to establish a protocol for in vitro propagation of Myrtle”

✓ located verbatim in the source · abstract, characters 270–328 · show in text ↑
e3

“adding combination of coconut water at 5% plus Banana pulp plus Papaya extract at 50 g/L of were helpful in maximizing number of shoots/plant, shoot length and greening parameters”

✓ located verbatim in the source · abstract, characters 811–990 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Duplicating the dose of vitamin mix of Gamborg medium improved explant development and survival.

Taxon
Myrtus communis L.Myrtus communist0
Treatment
duplicating the dose of vitamin mix of Gamborg mediume4
Outcome
explants development and survival (%)e4
Direction
improvede4
Setting
in vitroe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Myrtus communis
Myrtus communis · TaxonGeorge E. Koronaios · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

t0

“Myrtus communis L.”

✓ located verbatim in the source · title, characters 63–81 · show in text ↑
e0

“to establish a protocol for in vitro propagation of Myrtle”

✓ located verbatim in the source · abstract, characters 270–328 · show in text ↑
e4

“duplicating the dose of vitamin mix of Gamborg medium improved explants development and survival (%) of explant”

✓ located verbatim in the source · abstract, characters 997–1108 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

1.0 mg/L BAP increased explant proliferation.

Taxon
Myrtus communis L.Myrtus communist0
Treatment
1.0 mg/ L BAPe5
Outcome
proliferatione5
Direction
increasede5
Setting
in vitroe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Myrtus communis
Myrtus communis · TaxonGeorge E. Koronaios · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

t0

“Myrtus communis L.”

✓ located verbatim in the source · title, characters 63–81 · show in text ↑
e0

“to establish a protocol for in vitro propagation of Myrtle”

✓ located verbatim in the source · abstract, characters 270–328 · show in text ↑
e5

“using of 1.0 mg/ L BAP increased proliferation”

✓ located verbatim in the source · abstract, characters 1121–1167 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

2.0 mg/L GA3 added to half strength medium maximized shoot length.

Taxon
Myrtus communis L.Myrtus communist0
Treatment
2.0 mg/ L GA3e6
Outcome
shoot lengthe6
Direction
maximizede6
Qualifier
half strength mediume6
Setting
in vitroe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Myrtus communis
Myrtus communis · TaxonGeorge E. Koronaios · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

t0

“Myrtus communis L.”

✓ located verbatim in the source · title, characters 63–81 · show in text ↑
e0

“to establish a protocol for in vitro propagation of Myrtle”

✓ located verbatim in the source · abstract, characters 270–328 · show in text ↑
e6

“addition of 2.0 mg/ L GA3 to half strength medium maximized shoot length”

✓ located verbatim in the source · abstract, characters 1180–1252 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

2.0 mg/L IBA induced the highest number of roots per plant.

Taxon
Myrtus communis L.Myrtus communist0
Treatment
2.0 mg/L IBAe7
Outcome
number of roots/plante7
Direction
higheste7
Setting
in vitroe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Myrtus communis
Myrtus communis · TaxonGeorge E. Koronaios · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

t0

“Myrtus communis L.”

✓ located verbatim in the source · title, characters 63–81 · show in text ↑
e0

“to establish a protocol for in vitro propagation of Myrtle”

✓ located verbatim in the source · abstract, characters 270–328 · show in text ↑
e7

“the addition of 2.0 mg/L IBA to the culture medium induced the highest number of roots/plant”

✓ located verbatim in the source · abstract, characters 1264–1356 · show in text ↑

indole-3-butyric acid · growth · Myrtus communisResults agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.