Paper · 2025 · Horticulturae

Bacillus thuringiensis and Trichoderma asperellum as Biostimulants in Hydroponic Tendril Pea (Pisum sativum) Microgreens

Ramona Pérez-Leal; María Janeth Rodríguez-Roque; Angélica Anahí Acevedo-Barrera; et al.

8
claims
18
verbatim quotes
4
citations
3
research areas
✓
Open access · cc-by

Abstract

The study evaluated the effect of Bacillus thuringiensis (Bt) and Trichoderma asperellum (Ta) as biostimulants in hydroponically grown tendril pea (Pisum sativum) microgreens. A completely randomized experimental design was conducted under controlled conditions; the study included the root pea-spraying treatment with Bt, Ta, and their combination, alongside a non-inoculated control. The results showed that treatments with Ta significantly enhanced growth parameters, such as stem length and diameter, stipular leaf area, and fresh weight of the microgreens. Specifically, fresh biomass increased by 140% and dry biomass by 80% with Ta compared to the control, while combined treatment with Bt and Ta reduced nitrates by 39.6%. Bt and Bt + Ta increased chlorophyll b and carotenoids contents, suggesting improved photosynthetic activity. No significant differences in visual appearance were observed. In conclusion, the application of Ta and Bt can enhance tendril pea microgreens’ yield and certain biochemical (chlorophyll b and carotenoids) qualities without affecting their visual quality, supporting the application of these microorganisms as biostimulants. These findings underscore the potential to scale these treatments in commercial hydroponic systems, offering a sustainable approach to optimizing the production of this emerging crop type.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Trichoderma asperellum significantly enhanced growth parameters (stem length and diameter, stipular leaf area) of tendril pea microgreens relative to the non-inoculated control.

Taxon
tendril pea (Pisum sativum)Lathyrus oleraceuse0
Treatment
Trichoderma asperellume0
Interactor
Trichoderma asperellumTrichoderma asperellume0other
Outcome
stem length and diameter, stipular leaf areae2
Direction
significantly enhancede2
Comparator
non-inoculated controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗
Trichoderma asperellum
Trichoderma asperellum · InteractorTashkoskip · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The study evaluated the effect of Bacillus thuringiensis (Bt) and Trichoderma asperellum (Ta) as biostimulants in hydroponically grown tendril pea (Pisum sativum) microgreens”

✓ located verbatim in the source · abstract, characters 0–174 · show in text ↑
e1

“alongside a non-inoculated control”

✓ located verbatim in the source · abstract, characters 350–384 · show in text ↑
e2

“treatments with Ta significantly enhanced growth parameters, such as stem length and diameter, stipular leaf area, and fresh weight of the microgreens”

✓ located verbatim in the source · abstract, characters 410–560 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma asperellum increased fresh biomass of tendril pea microgreens by 140% compared with the control.

Taxon
tendril pea (Pisum sativum)Lathyrus oleraceuse0
Treatment
Trichoderma asperellume0
Interactor
Trichoderma asperellumTrichoderma asperellume0other
Outcome
fresh biomasse3
Direction
increasede3
Value
140%e3
Comparator
controle3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗
Trichoderma asperellum
Trichoderma asperellum · InteractorTashkoskip · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The study evaluated the effect of Bacillus thuringiensis (Bt) and Trichoderma asperellum (Ta) as biostimulants in hydroponically grown tendril pea (Pisum sativum) microgreens”

✓ located verbatim in the source · abstract, characters 0–174 · show in text ↑
e3

“Specifically, fresh biomass increased by 140% and dry biomass by 80% with Ta compared to the control”

✓ located verbatim in the source · abstract, characters 562–662 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma asperellum increased dry biomass of tendril pea microgreens by 80% compared with the control.

Taxon
tendril pea (Pisum sativum)Lathyrus oleraceuse0
Treatment
Trichoderma asperellume0
Interactor
Trichoderma asperellumTrichoderma asperellume0other
Outcome
dry biomasse3
Direction
increasede3
Value
80%e3
Comparator
controle3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗
Trichoderma asperellum
Trichoderma asperellum · InteractorTashkoskip · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The study evaluated the effect of Bacillus thuringiensis (Bt) and Trichoderma asperellum (Ta) as biostimulants in hydroponically grown tendril pea (Pisum sativum) microgreens”

✓ located verbatim in the source · abstract, characters 0–174 · show in text ↑
e3

“Specifically, fresh biomass increased by 140% and dry biomass by 80% with Ta compared to the control”

✓ located verbatim in the source · abstract, characters 562–662 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Combined treatment with Bt and Ta reduced nitrates in tendril pea microgreens by 39.6%.

Taxon
tendril pea (Pisum sativum)Lathyrus oleraceuse0
Treatment
combined treatment with Bt and Tae4
Interactor
Bacillus thuringiensisBacillus thuringiensise0other
Second interactor
Trichoderma asperellumTrichoderma asperellume0other
Outcome
nitratese4
Direction
reducede4
Value
39.6%e4
Comparator
non-inoculated controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗
Bacillus thuringiensis
Bacillus thuringiensis · InteractorSam LaRussa · CC BY 4.0 · iNaturalist ↗
Trichoderma asperellum
Trichoderma asperellum · Second interactorTashkoskip · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The study evaluated the effect of Bacillus thuringiensis (Bt) and Trichoderma asperellum (Ta) as biostimulants in hydroponically grown tendril pea (Pisum sativum) microgreens”

✓ located verbatim in the source · abstract, characters 0–174 · show in text ↑
e1

“alongside a non-inoculated control”

✓ located verbatim in the source · abstract, characters 350–384 · show in text ↑
e4

“while combined treatment with Bt and Ta reduced nitrates by 39.6%”

✓ located verbatim in the source · abstract, characters 664–729 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Bt and Bt + Ta increased chlorophyll b and carotenoid contents of tendril pea microgreens.

Taxon
tendril pea (Pisum sativum)Lathyrus oleraceuse0
Treatment
Bt and Bt + Tae5
Interactor
Bacillus thuringiensisBacillus thuringiensise0other
Outcome
chlorophyll b and carotenoids contentse5
Direction
increasede5
Comparator
non-inoculated controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗
Bacillus thuringiensis
Bacillus thuringiensis · InteractorSam LaRussa · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The study evaluated the effect of Bacillus thuringiensis (Bt) and Trichoderma asperellum (Ta) as biostimulants in hydroponically grown tendril pea (Pisum sativum) microgreens”

✓ located verbatim in the source · abstract, characters 0–174 · show in text ↑
e1

“alongside a non-inoculated control”

✓ located verbatim in the source · abstract, characters 350–384 · show in text ↑
e5

“Bt and Bt + Ta increased chlorophyll b and carotenoids contents, suggesting improved photosynthetic activity”

✓ located verbatim in the source · abstract, characters 731–839 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Bt and Bt + Ta treatments suggest improved photosynthetic activity in tendril pea microgreens.

Taxon
tendril pea (Pisum sativum)Lathyrus oleraceuse0
Treatment
Bt and Bt + Tae5
Outcome
improved photosynthetic activitye5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The study evaluated the effect of Bacillus thuringiensis (Bt) and Trichoderma asperellum (Ta) as biostimulants in hydroponically grown tendril pea (Pisum sativum) microgreens”

✓ located verbatim in the source · abstract, characters 0–174 · show in text ↑
e5

“Bt and Bt + Ta increased chlorophyll b and carotenoids contents, suggesting improved photosynthetic activity”

✓ located verbatim in the source · abstract, characters 731–839 · show in text ↑
Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

No significant differences in visual appearance were observed among the treatments of tendril pea microgreens.

Taxon
tendril pea (Pisum sativum)Lathyrus oleraceuse0
Outcome
visual appearancee6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The study evaluated the effect of Bacillus thuringiensis (Bt) and Trichoderma asperellum (Ta) as biostimulants in hydroponically grown tendril pea (Pisum sativum) microgreens”

✓ located verbatim in the source · abstract, characters 0–174 · show in text ↑
e6

“No significant differences in visual appearance were observed”

✓ located verbatim in the source · abstract, characters 841–902 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Application of Ta and Bt can enhance yield and biochemical (chlorophyll b and carotenoids) qualities of tendril pea microgreens without affecting their visual quality.

Taxon
tendril pea microgreense7
Outcome
yield and certain biochemical (chlorophyll b and carotenoids) qualitiese7

Verbatim

e7

“the application of Ta and Bt can enhance tendril pea microgreens’ yield and certain biochemical (chlorophyll b and carotenoids) qualities without affecting their visual quality”

✓ located verbatim in the source · abstract, characters 919–1095 · show in text ↑

Trichoderma asperellum · growth · Lathyrus oleraceus (pea)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.