Abstract
Woronin bodies are unique organelles in Pezizomycotina fungi that allow hyphae compartmentalization and prevent cytoplasmatic bleeding after mechanical injury. Several studies have related the peroxisomal protein HEX1, the major component of Woronin bodies with other biological processes such as hyphal growth, osmotic stress tolerance and pathogenicity. Trichoderma spp. are plant-beneficial multipurpose biological control agents, and proteomic and transcriptomic studies have shown that HEX1 and its corresponding gene are overrepresented when grown in the presence of fungal cell walls and plant polymers. To further investigate the involvement of hex1 in Trichoderma biology, we generated hex1 deletion transformants using the wheat endophytic strain T. simmonsii T137 as host. Results confirmed that hex1 gene is involved in the prevention of cytoplasmatic bleeding, and also has a role in fungal growth and biocontrol potential against phytopathogenic fungi and oomycetes. The involvement of hex1 in the fungal response to osmotic and oxidative stresses is conditioned by the type of stress and by the nutrient richness of the culture medium. The hex1 deletion also affected the interaction with wheat, but did not affect the plant protective effect of T137 against water stress. Overall, this study shows the implication of HEX1 in a wide range of biological processes necessary for T. simmonsii to deploy its abilities to be used as an agriculturally beneficial fungus. • The deletion of hex1 in T. simmonsii T137 reduces its biocontrol potential • HEX1 is involved in the distinct fungal responses to oxidative and osmotic stresses • The role of HEX1 in fungal tolerance to abiotic stress is only observed in rich media • HEX1 is involved in T. simmonsii -wheat interactions but not in drought protection
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Claims 2
Claim · model-written summary
hex1 deletion affected the interaction of T. simmonsii with wheat.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- hex1 deletione0
- Outcome
- interaction with wheate0
Not reportedInteractorInteractor roleComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hex1 deletion also affected the interaction with wheat”
t0“Trichoderma simmonsii is involved in stress responses, biocontrol potential and wheat plant growth”
Claim · model-written summary
hex1 deletion did not affect T137 protection of wheat from water stress.
- Taxon
- wheatTriticum aestivume0
- Treatment
- hex1 deletione0
- Outcome
- plant protective effect of T137 against water stresse0
- Direction
- did not affecte0
Not reportedInteractorInteractor roleComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hex1 deletion also affected the interaction with wheat, but did not affect the plant protective effect of T137 against water stress”
t0“Trichoderma simmonsii is involved in stress responses, biocontrol potential and wheat plant growth”
The same questions in other papers
loss of function · control · Triticum aestivum (wheat)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.