Abstract
Verticillium wilt caused by the soil-borne fungus Verticillium dahliae Kleb., is a destructive plant disease that instigates severe losses in many crops. Improving plant resistance to Verticillium wilt has been a challenge in most crops. In this study, a V. dahliae secreted protein VdSP8 was identified and shown to activate hyper-sensitive response (HR) and systemic acquired resistance (SAR) to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and Botrytis cinerea in tobacco plants. We identified a β-glucosidase named GhBGLU46 as a cotton plant target of VdSP8. VdSP8 interacts with GhBGLU46 both in vivo and in vitro and promotes the β-glucosidase activity of GhBGLU46. Silencing of GhBGLU46 reduced the expression of genes involved in lignin biosynthesis, such as GhCCR4, GhCCoAOMT2, GhCAD3 and GhCAD6, thus decreasing lignin deposition and increasing Verticillium wilt susceptibility. We have shown that GhBGLU46 is indispensable for the function of VdSP8 in plant resistance. These results suggest that plants have also evolved a strategy to exploit the invading effector protein VdSP8 to enhance plant resistance.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
VdSP8 activated hypersensitive response in tobacco plants.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- VdSP8e0
- Interactor
- V. dahliae
Verticillium dahliaee0pathogen - Outcome
- hyper-sensitive response (HR)e0
- Direction
- activatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“V. dahliae secreted protein VdSP8 was identified and shown to activate hyper-sensitive response (HR) and systemic acquired resistance (SAR) to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and Botrytis cinerea in tobacco plants.”
Claim · model-written summary
VdSP8 activated systemic acquired resistance against Pst DC3000 in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- VdSP8e0
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e0pathogen
- Outcome
- systemic acquired resistance (SAR)e0
- Direction
- activatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“V. dahliae secreted protein VdSP8 was identified and shown to activate hyper-sensitive response (HR) and systemic acquired resistance (SAR) to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and Botrytis cinerea in tobacco plants.”
Claim · model-written summary
VdSP8 activated systemic acquired resistance against Botrytis cinerea in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- VdSP8e0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Outcome
- systemic acquired resistance (SAR)e0
- Direction
- activatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“V. dahliae secreted protein VdSP8 was identified and shown to activate hyper-sensitive response (HR) and systemic acquired resistance (SAR) to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and Botrytis cinerea in tobacco plants.”
Claim · model-written summary
GhBGLU46 was identified as a cotton target of VdSP8.
- Taxon
- cotton
Gossypium hirsutume0 - Gene
- GhBGLU46e0
- Outcome
- cotton plant target of VdSP8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified a β-glucosidase named GhBGLU46 as a cotton plant target of VdSP8.”
e1“V. dahliae secreted protein VdSP8”
Claim · model-written summary
VdSP8 interacted with GhBGLU46 in vivo and in vitro.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhBGLU46e0
- Outcome
- interacts with GhBGLU46e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VdSP8 interacts with GhBGLU46 both in vivo and in vitro”
t0“Cotton”
e1“V. dahliae secreted protein VdSP8”
Claim · model-written summary
VdSP8 promoted GhBGLU46 β-glucosidase activity.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- VdSP8e1
- Gene
- GhBGLU46e0
- Outcome
- β-glucosidase activity of GhBGLU46e0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promotes the β-glucosidase activity of GhBGLU46”
t0“Cotton”
e1“VdSP8 interacts with GhBGLU46”
Claim · model-written summary
GhBGLU46 silencing reduced expression of lignin biosynthesis genes.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- Silencing of GhBGLU46e0
- Gene
- GhBGLU46e0
- Outcome
- expression of genes involved in lignin biosynthesise0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of GhBGLU46 reduced the expression of genes involved in lignin biosynthesis, such as GhCCR4, GhCCoAOMT2, GhCAD3 and GhCAD6”
t0“Cotton”
Claim · model-written summary
GhBGLU46 silencing decreased lignin deposition.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- Silencing of GhBGLU46e0
- Gene
- GhBGLU46e0
- Outcome
- lignin depositione0
- Direction
- decreasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of GhBGLU46 reduced the expression of genes involved in lignin biosynthesis, such as GhCCR4, GhCCoAOMT2, GhCAD3 and GhCAD6, thus decreasing lignin deposition and increasing Verticillium wilt susceptibility.”
t0“Cotton”
Claim · model-written summary
GhBGLU46 silencing increased cotton susceptibility to Verticillium wilt.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- Silencing of GhBGLU46e0
- Interactor
- VerticilliumVerticilliume0pathogen
- Gene
- GhBGLU46e0
- Outcome
- Verticillium wilt susceptibilitye0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of GhBGLU46 reduced the expression of genes involved in lignin biosynthesis, such as GhCCR4, GhCCoAOMT2, GhCAD3 and GhCAD6, thus decreasing lignin deposition and increasing Verticillium wilt susceptibility.”
t0“Cotton”
Claim · model-written summary
GhBGLU46 was necessary for VdSP8-mediated plant resistance.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhBGLU46e0
- Outcome
- plant resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhBGLU46 is indispensable for the function of VdSP8 in plant resistance”
t0“Cotton”
e1“V. dahliae secreted protein VdSP8”
The same questions in other papers
loss of function · Verticillium · Gossypium hirsutum (cotton)Positive and negative results
7 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →loss of function · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
21 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
22 papers more in this group
See all 26 papers →loss of function · molecular: metabolites · Gossypium hirsutum (cotton)Positive and negative results
31 papers in the same direction as this paper · 8 in the opposite direction
- positive
- negative
- positive
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.