Abstract
MicroRNAs (miRNAs) modulate many biological processes through inactivation of specific mRNA targets such as those encoding transcription factors. A delicate spatial/temporal balance between specific miRNAs and their targets is central to achieving the appropriate biological outcomes. Somatic embryogenesis in cotton (Gossypium hirsutum), which goes through initial cellular dedifferentiation, callus proliferation, and somatic embryo development, is of great importance for both fundamental research and biotechnological applications. In this study, we characterize the function of the GhmiR157a-GhSPL10 miRNA-transcription factor module during somatic embryogenesis in cotton. We show that overexpression of GhSPL10, a target of GhmiR157a, increases free auxin and ethylene content and expression of associated signaling pathways, activates the flavonoid biosynthesis pathway, and promotes initial cellular dedifferentiation and callus proliferation. Inhibition of expression of the flavonoid synthesis gene F3H in GhSPL10 overexpression lines (35S:rSPL10-7) blocked callus initiation, while exogenous application of several types of flavonol promoted callus proliferation, associated with cell cycle-related gene expression. Inhibition of ethylene synthesis by aminoethoxyvinylglycine treatment in the 35S:rSPL10-7 line severely inhibited callus initiation, while activation of ethylene signaling through 1-aminocyclopropane 1-carboxylic acid treatment, EIN2 overexpression, or inhibition of the ethylene negative regulator CTR1 by RNA interference promoted flavonoid-related gene expression and flavonol accumulation. These results show that an up-regulation of ethylene signaling and the activation of flavonoid biosynthesis in GhSPL10 overexpression lines were associated with initial cellular dedifferentiation and callus proliferation. Our results demonstrate the importance of a GhmiR157a-GhSPL10 gene module in regulating somatic embryogenesis via hormonal and flavonoid pathways.
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Claims 11
Claim · model-written summary
GhSPL10 overexpression raised free auxin and ethylene content.
- Taxon
- cotton
Gossypium hirsutumt0 - Treatment
- overexpression of GhSPL10e0
- Gene
- GhSPL10e0
- Outcome
- free auxin and ethylene contente0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GhSPL10, a target of GhmiR157a, increases free auxin and ethylene content”
t0“cotton”
Claim · model-written summary
GhSPL10 overexpression increased expression of associated signaling pathways.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- overexpression of GhSPL10e0
- Gene
- GhSPL10e0
- Outcome
- expression of associated signaling pathwayse0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GhSPL10, a target of GhmiR157a, increases free auxin and ethylene content and expression of associated signaling pathways”
t0“cotton”
Claim · model-written summary
GhSPL10 overexpression activated flavonoid biosynthesis.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- overexpression of GhSPL10e0
- Gene
- GhSPL10e0
- Outcome
- flavonoid biosynthesis pathwaye0
- Direction
- activatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GhSPL10, a target of GhmiR157a, increases free auxin and ethylene content and expression of associated signaling pathways, activates the flavonoid biosynthesis pathway”
t0“cotton”
Claim · model-written summary
GhSPL10 overexpression promoted initial dedifferentiation and callus proliferation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- overexpression of GhSPL10e0
- Gene
- GhSPL10e0
- Outcome
- initial cellular dedifferentiation and callus proliferatione0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GhSPL10, a target of GhmiR157a, increases free auxin and ethylene content and expression of associated signaling pathways, activates the flavonoid biosynthesis pathway, and promotes initial cellular dedifferentiation and callus proliferation”
t0“cotton”
Claim · model-written summary
F3H inhibition blocked callus initiation in GhSPL10 overexpression lines.
- Taxon
- cottonGossypium hirsutumt0
- Material
- 35S:rSPL10-7e0
- Treatment
- Inhibition of expression of the flavonoid synthesis gene F3He0
- Gene
- F3He0
- Outcome
- callus initiatione0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of expression of the flavonoid synthesis gene F3H in GhSPL10 overexpression lines (35S:rSPL10-7) blocked callus initiation”
t0“cotton”
Claim · model-written summary
Exogenous flavonol promoted callus proliferation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- exogenous application of several types of flavonole0
- Outcome
- callus proliferatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous application of several types of flavonol promoted callus proliferation, associated with cell cycle-related gene expression”
t0“cotton”
Claim · model-written summary
Flavonol-promoted callus proliferation was associated with cell cycle gene expression.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- cell cycle-related gene expressione0
- Outcome
- callus proliferatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“flavonol promoted callus proliferation, associated with cell cycle-related gene expression”
t0“cotton”
Claim · model-written summary
Inhibiting ethylene synthesis with aminoethoxyvinylglycine severely inhibited callus initiation.
- Taxon
- cottonGossypium hirsutumt0
- Material
- 35S:rSPL10-7e0
- Treatment
- aminoethoxyvinylglycine treatmente0
- Outcome
- callus initiatione0
- Direction
- severely inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of ethylene synthesis by aminoethoxyvinylglycine treatment in the 35S:rSPL10-7 line severely inhibited callus initiation”
t0“cotton”
Claim · model-written summary
ACC treatment promoted flavonoid-related gene expression and flavonol accumulation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- 1-aminocyclopropane 1-carboxylic acid treatmente0
- Outcome
- flavonoid-related gene expression and flavonol accumulatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1-aminocyclopropane 1-carboxylic acid treatment, EIN2 overexpression, or inhibition of the ethylene negative regulator CTR1 by RNA interference promoted flavonoid-related gene expression and flavonol accumulation”
t0“cotton”
Claim · model-written summary
EIN2 overexpression promoted flavonoid-related gene expression and flavonol accumulation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- EIN2 overexpressione0
- Gene
- EIN2e0
- Outcome
- flavonoid-related gene expression and flavonol accumulatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EIN2 overexpression, or inhibition of the ethylene negative regulator CTR1 by RNA interference promoted flavonoid-related gene expression and flavonol accumulation”
t0“cotton”
Claim · model-written summary
CTR1 RNA interference promoted flavonoid-related gene expression and flavonol accumulation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- inhibition of the ethylene negative regulator CTR1 by RNA interferencee0
- Gene
- CTR1e0
- Outcome
- flavonoid-related gene expression and flavonol accumulatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of the ethylene negative regulator CTR1 by RNA interference promoted flavonoid-related gene expression and flavonol accumulation”
t0“cotton”
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See all 26 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.