Abstract
Despite the crucial role of microbial communities in agroecosystem functioning, a clear picture of how nitrogen shapes rhizosphere microbial complexity and community structure across diverse maize lines remains elusive. To address this gap, we conducted 16S amplicon sequencing of the rhizosphere microbial communities across a diverse range of maize inbred lines (305 genotypes) and their F1 hybrids (196 genotypes) cultivated in both low-nitrogen (unfertilized) and high-nitrogen (fertilized) soils. Our findings reveal that N fertilizer treatment had contrasting effects on the rhizosphere microbial communities of inbreds and hybrids. N fertilization increased alpha diversity but decreased the abundance of Pseudomonas taxa in inbred lines, while the opposite was true for hybrids. The proportion of variance determined by plant host factors was also better explained under low-N, demonstrating that N fertilization reduced the influence of the host over the rhizosphere microbial community. Microbial networks revealed significant differences in the number of nodes and clustering coefficients between the rhizosphere microbial communities of inbred and hybrid maize, with these differences being further differentiated by changes in nitrogen levels. Overall, our study reveals the interplay among rhizosphere microbiomes, abiotic stress induced by low soil nitrogen, and plant host factors facilitating the identification of stable microbial communities in response to environmental stress. These findings contribute to the potential engineering of resilient microbial consortia highlighting the importance of the influence of plant genotype and the environment on the rhizosphere microbiome.
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Claims 6
Claim · model-written summary
Nitrogen fertilization increased rhizosphere microbial alpha diversity in maize inbred lines.
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N fertilization increased alpha diversity but decreased the abundance of Pseudomonas taxa in inbred lines”
t0“maize lines”
Claim · model-written summary
Nitrogen fertilization decreased Pseudomonas abundance in maize inbred lines.
- Material
- inbred linese0
- Treatment
- N fertilizatione0
- Interactor
- Pseudomonas
Pseudomonase0symbiont - Outcome
- abundance of Pseudomonas taxae0
- Direction
- decreasede0
Not reportedTaxonComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N fertilization increased alpha diversity but decreased the abundance of Pseudomonas taxa in inbred lines”
Claim · model-written summary
In maize hybrids, nitrogen fertilization decreased alpha diversity.
Not reportedTaxonComparator
Verbatim
e0“N fertilization increased alpha diversity but decreased the abundance of Pseudomonas taxa in inbred lines, while the opposite was true for hybrids”
Claim · model-written summary
In maize hybrids, nitrogen fertilization increased Pseudomonas abundance.
- Material
- hybridse0
- Treatment
- N fertilizatione0
- Interactor
- PseudomonasPseudomonase0symbiont
- Outcome
- abundance of Pseudomonas taxae0
- Direction
- increasede0
Not reportedTaxonComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N fertilization increased alpha diversity but decreased the abundance of Pseudomonas taxa in inbred lines, while the opposite was true for hybrids”
Claim · model-written summary
Host factors explained more rhizosphere community variance under low nitrogen than with fertilization.
- Treatment
- N fertilizatione0
- Outcome
- proportion of variance determined by plant host factorse0
- Comparator
- low-Ne0
Not reportedTaxonDirection
Verbatim
e0“The proportion of variance determined by plant host factors was also better explained under low-N, demonstrating that N fertilization reduced the influence of the host over the rhizosphere microbial community”
Claim · model-written summary
Inbred and hybrid maize rhizosphere networks differed significantly in node number and clustering coefficients, with nitrogen levels further differentiating them.
- Material
- inbred and hybrid maizee0
- Treatment
- changes in nitrogen levelse0
- Outcome
- number of nodes and clustering coefficientse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Microbial networks revealed significant differences in the number of nodes and clustering coefficients between the rhizosphere microbial communities of inbred and hybrid maize, with these differences being further differentiated by changes in nitrogen levels”
The same questions in other papers
fertilization · ecology: diversity · Zea mays (maize)Positive and negative results
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- no effect
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.