Abstract
Two proteins of the citrus tatter leaf virus (CTLV), a strain of the apple stem grooving virus (ASGV), capable of inducing citrus bud union disorders on commercially important trifoliate and citrange rootstocks, were identified as viral suppressors of RNA silencing (VSR). Both the coat protein (CP) and the movement protein (MP) suppressed RNA silencing in GFP-transgenic Nicotiana benthamiana 16c plants in agrobacterium-mediated co-infiltration assays; the MP acted as a local VSR, while the CP acted as a systemic VSR. When the potato virus X (PVX) infectious vector harbored either the CTLV CP or MP gene, viral infection and symptom development were promoted in N. benthamiana . Deletions of amino acids in the CP sequence or the MP sequence resulted in failure to promote PVX infections as well as suppression of silencing in agrobacterium-mediated co-infiltration assays. Mass spectrometry-based immunoprecipitation proteomics showed that neither the CTLV CP nor the MP interacts with cellular components directly involved in host antiviral RNA silencing pathways. RNA immunoprecipitation (RIP) and RNA-protein pull-down assays indicated that the CTLV MP interacts with double-stranded RNA (dsRNA) presumably through a protein complex or proteins containing RNA binding domains. It is possible that the MP prevents dsRNA cleavage through this mechanism, leading to suppression of host antiviral RNA silencing. These findings confirmed that CTLV uses VSRs as part of its overall strategy to overcome host antiviral defenses and are indicative of the ability of ASGV and CTLV to infect a wide range of hosts including different species of woody and herbaceous plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
CTLV coat protein suppressed RNA silencing systemically in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- coat protein (CP)e0
- Gene
- coat protein (CP)e0
- Outcome
- RNA silencinge0
- Direction
- suppressede0
- Qualifier
- systemic VSRe0
Not reportedInteractorComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the coat protein (CP) and the movement protein (MP) suppressed RNA silencing in GFP-transgenic Nicotiana benthamiana 16c plants in agrobacterium-mediated co-infiltration assays; the MP acted as a local VSR, while the CP acted as a systemic VSR.”
Claim · model-written summary
CTLV movement protein suppressed RNA silencing locally in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- movement protein (MP)e0
- Gene
- movement protein (MP)e0
- Outcome
- RNA silencinge0
- Direction
- suppressede0
- Qualifier
- local VSRe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the coat protein (CP) and the movement protein (MP) suppressed RNA silencing in GFP-transgenic Nicotiana benthamiana 16c plants in agrobacterium-mediated co-infiltration assays; the MP acted as a local VSR, while the CP acted as a systemic VSR.”
Claim · model-written summary
PVX carrying CTLV CP promoted viral infection and symptoms in Nicotiana benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- potato virus X (PVX) infectious vectore0
- Interactor
- potato virus X (PVX)Potato virus Xe0pathogen
- Gene
- CTLV CPe0
- Outcome
- viral infection and symptom developmente0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the potato virus X (PVX) infectious vector harbored either the CTLV CP or MP gene, viral infection and symptom development were promoted in N. benthamiana .”
Claim · model-written summary
PVX carrying CTLV MP promoted viral infection and symptoms in Nicotiana benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- potato virus X (PVX) infectious vectore0
- Interactor
- potato virus X (PVX)Potato virus Xe0pathogen
- Gene
- MP genee0
- Outcome
- viral infection and symptom developmente0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the potato virus X (PVX) infectious vector harbored either the CTLV CP or MP gene, viral infection and symptom development were promoted in N. benthamiana .”
Claim · model-written summary
Deleting amino acids from CP prevented promotion of PVX infection and silencing suppression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Deletions of amino acids in the CP sequencee0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- CP sequencee0
- Outcome
- PVX infections as well as suppression of silencinge0
- Direction
- failure to promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletions of amino acids in the CP sequence or the MP sequence resulted in failure to promote PVX infections as well as suppression of silencing in agrobacterium-mediated co-infiltration assays.”
e1“Nicotiana benthamiana 16c plants”
Claim · model-written summary
Deleting amino acids from MP prevented promotion of PVX infection and silencing suppression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Deletions of amino acids in the CP sequence or the MP sequencee0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- MP sequencee0
- Outcome
- PVX infections as well as suppression of silencinge0
- Direction
- failure to promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletions of amino acids in the CP sequence or the MP sequence resulted in failure to promote PVX infections as well as suppression of silencing in agrobacterium-mediated co-infiltration assays.”
e1“Nicotiana benthamiana 16c plants”
Claim · model-written summary
Neither CTLV CP nor MP interacted directly with host antiviral silencing components.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- CTLV CP nor the MPe0
- Outcome
- interacts with cellular components directly involved in host antiviral RNA silencing pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“neither the CTLV CP nor the MP interacts with cellular components directly involved in host antiviral RNA silencing pathways.”
e1“Nicotiana benthamiana 16c plants”
Claim · model-written summary
CTLV MP interacted with dsRNA, possibly through an RNA-binding protein complex.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- CTLV MPe0
- Outcome
- interacts with double-stranded RNA (dsRNA)e0
- Qualifier
- presumably through a protein complex or proteins containing RNA binding domainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the CTLV MP interacts with double-stranded RNA (dsRNA) presumably through a protein complex or proteins containing RNA binding domains.”
e1“Nicotiana benthamiana 16c plants”
Claim · model-written summary
The authors propose MP may prevent dsRNA cleavage, suppressing host antiviral RNA silencing.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- MPe0
- Outcome
- suppression of host antiviral RNA silencinge0
- Qualifier
- prevents dsRNA cleavagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is possible that the MP prevents dsRNA cleavage through this mechanism, leading to suppression of host antiviral RNA silencing.”
e1“Nicotiana benthamiana 16c plants”
The same questions in other papers
Solanum tuberosum · Potato virus X · Nicotiana benthamianaPositive and negative results
22 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
21 papers more in this group
See all 25 papers →loss of function · Potato virus X · Nicotiana benthamianaPositive and negative results
7 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
- negative
11 papers more in this group
See all 15 papers →infection · Potato virus X · Nicotiana benthamianaResults agree
5 papers in the same direction as this paper
- negative
- negative
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.