Abstract
Nucleotide binding leucine-rich repeat (NB-LRR) proteins play an important role in plant and mammalian innate immunity. In plants, these resistance proteins recognize specific pathogen-derived effector proteins. Recognition subsequently triggers a rapid and efficient defense response often associated with the hypersensitive response and other poorly understood processes that suppress the pathogen. To investigate mechanisms associated with the activation of disease resistance responses, we investigated proteins binding to the potato (Solanum tuberosum) NB-LRR protein Rx that confers extreme resistance to Potato virus X (PVX) in potato and Nicotiana benthamiana. By affinity purification experiments, we identified an endogenous N. benthamiana Ran GTPase-Activating Protein2 (RanGAP2) as an Rx-associated protein in vivo. Further characterization confirmed the specificity of this interaction and showed that the association occurs through their N-terminal domains. By specific virus-induced gene silencing of RanGAP2 in N. benthamiana carrying Rx, we demonstrated that this interaction is required for extreme resistance to PVX and suggest that RanGAP2 is part of the Rx signaling complex. These results implicate RanGAP-mediated cellular mechanisms, including nucleocytoplasmic trafficking, in the activation of disease resistance.
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Claims 6
Claim · model-written summary
N. benthamiana RanGAP2 was identified as an Rx-associated protein in vivo.
- Taxon
- N. benthamiana
Nicotiana benthamianae0 - Gene
- Ran GTPase-Activating Protein2 (RanGAP2)e0
- Outcome
- Rx-associated protein in vivoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified an endogenous N. benthamiana Ran GTPase-Activating Protein2 (RanGAP2) as an Rx-associated protein in vivo”
Claim · model-written summary
The RanGAP2–Rx interaction was specific.
- Taxon
- N. benthamianaNicotiana benthamianae2
- Gene
- RanGAP2e1
- Outcome
- specificity of this interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Further characterization confirmed the specificity of this interaction”
e1“Ran GTPase-Activating Protein2 (RanGAP2) as an Rx-associated protein”
e2“N. benthamiana Ran GTPase-Activating Protein2”
Claim · model-written summary
Rx and RanGAP2 associated through their N-terminal domains.
- Taxon
- N. benthamianaNicotiana benthamianae2
- Gene
- RanGAP2e1
- Outcome
- association occurs through their N-terminal domainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the association occurs through their N-terminal domains”
e1“Ran GTPase-Activating Protein2 (RanGAP2) as an Rx-associated protein”
e2“N. benthamiana Ran GTPase-Activating Protein2”
Claim · model-written summary
Silencing RanGAP2 in Rx-carrying N. benthamiana showed it is required for extreme resistance to PVX.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- virus-induced gene silencing of RanGAP2e0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- RanGAP2e0
- Outcome
- extreme resistance to PVXe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By specific virus-induced gene silencing of RanGAP2 in N. benthamiana carrying Rx, we demonstrated that this interaction is required for extreme resistance to PVX”
e1“Potato virus X (PVX)”
Claim · model-written summary
The authors suggested RanGAP2 is part of the Rx signaling complex.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Gene
- RanGAP2e0
- Outcome
- Rx signaling complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggest that RanGAP2 is part of the Rx signaling complex”
e1“N. benthamiana carrying Rx”
Claim · model-written summary
The authors implicated RanGAP-mediated processes, including nucleocytoplasmic trafficking, in disease-resistance activation.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Outcome
- activation of disease resistancee0
- Qualifier
- nucleocytoplasmic traffickinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results implicate RanGAP-mediated cellular mechanisms, including nucleocytoplasmic trafficking, in the activation of disease resistance”
e1“N. benthamiana carrying Rx”
The same questions in other papers
loss of function · Potato virus X · Nicotiana benthamianaPositive and negative results
6 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.