Paper · 2023 · Asian Journal of Agriculture and Allied Sciences

In vitro Micropropagation of Vitis vinifera L. var. Cabernet Franc and Callus Production

Yanina S. García; Evelyn A. González; Olga M. Ruiz; et al.

7
claims
14
verbatim quotes
1
citations
4
research areas
—
Not open access

Abstract

The objective of this study was to produce in vitro-cultured mother plants of Cabernet Franc. Uninodal cuttings were taken from the plants, disinfected and sown in 50% Murashige and Skoog (MS) medium with the addition of 0.01% indoleacetic acid (IAA). Explant establishment, rooting, and leaf number were recorded and statistically analyzed after eight weeks of growth. The ex-vitro behavior of the plants was analyzed after four weeks of acclimatization under appropriate conditions of humidity and temperature in a 1:1 mixture of perlite: sterile fertile soil. The result showed that it is an optimal variety for in vitro culture, with mean values of 88% of establishment and 79% of rooting, production of 5 leaves per cutting and 100% of ex vitro acclimatization. Genetic material with good yield, health and acclimatization was obtained from the in vitro production of mother plants of Cabernet Franc. The Cabernet Franc explants formed callus after 15 days of exposure to 2,4-D. This indicates that it is an optimal material for genetic improvement.

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Claims 7

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Cabernet Franc uninodal cuttings reached a mean in vitro explant establishment of 88%.

Taxon
Vitis vinifera L.Vitis viniferat0
Material
Cabernet Franct0
Outcome
establishmente1
Value
88%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis vinifera
Vitis vinifera · TaxonDarijanus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“88% of establishment”

✓ located verbatim in the source · abstract, characters 653–673 · show in text ↑
t0

“Vitis vinifera L. var. Cabernet Franc”

✓ located verbatim in the source · title, characters 29–66 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Cabernet Franc cuttings reached a mean rooting of 79% in vitro.

Taxon
Vitis vinifera L.Vitis viniferat0
Material
Cabernet Franct0
Outcome
rootinge1
Value
79%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis vinifera
Vitis vinifera · TaxonDarijanus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“79% of rooting”

✓ located verbatim in the source · abstract, characters 678–692 · show in text ↑
t0

“Vitis vinifera L. var. Cabernet Franc”

✓ located verbatim in the source · title, characters 29–66 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

In vitro Cabernet Franc cuttings produced 5 leaves per cutting.

Taxon
Vitis vinifera L.Vitis viniferat0
Material
Cabernet Franct0
Outcome
leaves per cuttinge1
Value
5e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis vinifera
Vitis vinifera · TaxonDarijanus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“5 leaves per cutting”

✓ located verbatim in the source · abstract, characters 708–728 · show in text ↑
t0

“Vitis vinifera L. var. Cabernet Franc”

✓ located verbatim in the source · title, characters 29–66 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Ex vitro acclimatization of the in vitro Cabernet Franc plants reached 100%.

Taxon
Vitis vinifera L.Vitis viniferat0
Material
Cabernet Franct0
Outcome
ex vitro acclimatizatione1
Value
100%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis vinifera
Vitis vinifera · TaxonDarijanus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“100% of ex vitro acclimatization”

✓ located verbatim in the source · abstract, characters 733–765 · show in text ↑
t0

“Vitis vinifera L. var. Cabernet Franc”

✓ located verbatim in the source · title, characters 29–66 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cabernet Franc explants formed callus after 15 days of exposure to 2,4-D.

Taxon
Vitis vinifera L.Vitis viniferat0
Material
Cabernet France1
Treatment
2,4-De1
Outcome
formed calluse1
Qualifier
after 15 dayse1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis vinifera
Vitis vinifera · TaxonDarijanus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“The Cabernet Franc explants formed callus after 15 days of exposure to 2,4-D”

✓ located verbatim in the source · abstract, characters 906–982 · show in text ↑
t0

“Vitis vinifera L. var. Cabernet Franc”

✓ located verbatim in the source · title, characters 29–66 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Genetic material of Cabernet Franc with good yield, health and acclimatization was obtained from in vitro production of mother plants.

Taxon
Vitis vinifera L.Vitis viniferat0
Outcome
Genetic material with good yield, health and acclimatizatione1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis vinifera
Vitis vinifera · TaxonDarijanus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Genetic material with good yield, health and acclimatization was obtained from the in vitro production of mother plants of Cabernet Franc”

✓ located verbatim in the source · abstract, characters 767–904 · show in text ↑
t0

“Vitis vinifera L. var. Cabernet Franc”

✓ located verbatim in the source · title, characters 29–66 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors conclude that Cabernet Franc is an optimal material for genetic improvement.

Taxon
Vitis vinifera L.Vitis viniferat0
Outcome
optimal material for genetic improvemente1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis vinifera
Vitis vinifera · TaxonDarijanus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“This indicates that it is an optimal material for genetic improvement”

✓ located verbatim in the source · abstract, characters 984–1053 · show in text ↑
t0

“Vitis vinifera L. var. Cabernet Franc”

✓ located verbatim in the source · title, characters 29–66 · show in text ↑

2,4-D · growth · Vitis vinifera (wine grape)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.