Abstract
Since the last century, it has been shown that dedifferentiated cells of Camellia sinensis can produce catechins and other secondary metabolites under in vitro conditions, with potential applications in the cosmetic, pharmaceutical and food industries. In this work, cell suspension cultures of a C. sinensis cell line (LSC-5Y) were established in a liquid medium in order to optimize the biomass productivity, catechin monomer (GC, EGC, C, EC, CG, and ECG) and alkaloid (TB and CAF) productivity. The following factors were evaluated: concentration of growth regulators (BA and IBA), inoculum size, age of the cell line, light exposure, and effect of biotic elicitors (MeJA and extracts of Ciborinia camelliae). GC, EGC, and ECG increased approximately 1.80-fold when the auxin IBA concentration was increased from 0.1 to 2.0 mg/L. In addition, better productivity of EGC, C, EC, and CAF was achieved by using inoculum densities between 50 and 100 g/L. Although lower inoculum densities (25 g/L) showed a higher growth rate (0.20 d−1), the use of inoculum densities higher than 25 g/L favors a 2–4-fold increase in total catechin (TC) productivity, with maximum productivity being reached after 21 days of culture. However, the cell line showed instability in TC productivity: in the short term (in three successive subcultures), the coefficient of variation was 32.80%, and catechin production capacity was 2.5 years with maximum productivity at 0.5 years. Finally, it was observed that ethanol, used as an elicitor solvent, has a strong elicitor effect capable of increasing the accumulation of catechins up to 5.24 times compared to the treatment without an elicitor.
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Claims 8
Claim · model-written summary
Increasing IBA raised GC, EGC, and ECG approximately 1.80-fold.
- Taxon
- Camellia sinensis
Camellia sinensise0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GC, EGC, and ECG increased approximately 1.80-fold when the auxin IBA concentration was increased from 0.1 to 2.0 mg/L.”
t0“Camellia sinensis”
Claim · model-written summary
Inoculum densities of 50–100 g/L gave better EGC, C, EC, and CAF productivity.
- Taxon
- Camellia sinensisCamellia sinensise0t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“better productivity of EGC, C, EC, and CAF was achieved by using inoculum densities between 50 and 100 g/L.”
t0“Camellia sinensis”
Claim · model-written summary
Inoculum density of 25 g/L had a higher growth rate of 0.20 d−1.
- Taxon
- Camellia sinensisCamellia sinensise0t0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lower inoculum densities (25 g/L) showed a higher growth rate (0.20 d−1)”
t0“Camellia sinensis”
Claim · model-written summary
Densities above 25 g/L increased total catechin productivity two to fourfold.
- Taxon
- Camellia sinensisCamellia sinensise0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the use of inoculum densities higher than 25 g/L favors a 2–4-fold increase in total catechin (TC) productivity”
t0“Camellia sinensis”
Claim · model-written summary
Maximum total catechin productivity occurred after 21 days.
- Taxon
- Camellia sinensisCamellia sinensise0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum productivity being reached after 21 days of culture.”
t0“Camellia sinensis”
Claim · model-written summary
Total catechin productivity varied 32.80% across three successive subcultures.
- Taxon
- Camellia sinensisCamellia sinensise0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the short term (in three successive subcultures), the coefficient of variation was 32.80%”
t0“Camellia sinensis”
Claim · model-written summary
Catechin production capacity lasted 2.5 years, peaking at 0.5 years.
- Taxon
- Camellia sinensisCamellia sinensise0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“catechin production capacity was 2.5 years with maximum productivity at 0.5 years.”
t0“Camellia sinensis”
Claim · model-written summary
Ethanol increased catechin accumulation up to 5.24 times relative to no elicitor.
- Taxon
- Camellia sinensisCamellia sinensise0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethanol, used as an elicitor solvent, has a strong elicitor effect capable of increasing the accumulation of catechins up to 5.24 times compared to the treatment without an elicitor.”
t0“Camellia sinensis”
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