Abstract
Abstract In the ongoing arms race between rice and Magnaporthe oryzae , the pathogen employs effectors to evade the immune response, while the host develops resistance genes to recognise these effectors and confer resistance. In this study, we identified a novel Pik allele, Pik‐W25 , from wild rice WR25 through bulked‐segregant analysis, creating the Pik‐W25 NIL (Near‐isogenic Lines) named G9. Pik‐W25 conferred resistance to isolates expressing AvrPik‐ C/D/E alleles. CRISPR‐Cas9 editing was used to generate transgenic lines with a loss of function in Pik‐W25‐1 and Pik‐W25‐2 , resulting in loss of resistance in G9 to isolates expressing the three alleles, confirming that Pik‐W25‐induced immunity required both Pik‐W25‐1 and Pik‐W25‐2 . Yeast two‐hybrid (Y 2 H) and split luciferase complementation assays showed interactions between Pik‐W25‐1 and the three alleles, while Pik‐W25‐2 could not interact with AvrPik‐C, ‐D, and ‐ E alleles with Y 2 H assay, indicating Pik‐W25‐1 acts as an adaptor and Pik‐W25‐2 transduces the signal to trigger resistance. The Pik‐W25 NIL exhibited enhanced field resistance to leaf and panicle blast without significant changes in morphology or development compared to the parent variety CO39, suggesting its potential for resistance breeding. These findings advance our knowledge of rice blast resistance mechanisms and offer valuable resources for effective and sustainable control strategies.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Pik-W25 was identified in wild rice WR25 and a near-isogenic line G9 was created.
Verbatim
e0“we identified a novel Pik allele, Pik‐W25 , from wild rice WR25 through bulked‐segregant analysis, creating the Pik‐W25 NIL (Near‐isogenic Lines) named G9”
Claim · model-written summary
Pik-W25 conferred resistance to isolates carrying three AvrPik alleles.
- Taxon
- rice
Oryza sativat0 - Interactor
- isolatese0pathogen
- Gene
- Pik‐W25e0
- Outcome
- resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pik‐W25 conferred resistance to isolates expressing AvrPik‐ C/D/E alleles”
t0“rice blast”
Claim · model-written summary
Disrupting Pik-W25-1 and Pik-W25-2 removed G9 resistance to the three isolate types.
- Taxon
- riceOryza sativat0
- Treatment
- CRISPR‐Cas9 editinge0
- Interactor
- isolatese0pathogen
- Gene
- Pik‐W25‐1 and Pik‐W25‐2e0
- Outcome
- resistancee0
- Direction
- loss of resistancee0
- Comparator
- G9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR‐Cas9 editing was used to generate transgenic lines with a loss of function in Pik‐W25‐1 and Pik‐W25‐2 , resulting in loss of resistance in G9 to isolates expressing the three alleles”
t0“rice blast”
Claim · model-written summary
Pik-W25-1 interacted with all three alleles in binding assays.
- Taxon
- riceOryza sativat0
- Gene
- Pik‐W25‐1e0
- Outcome
- interactions between Pik‐W25‐1 and the three allelese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Yeast two‐hybrid (Y 2 H) and split luciferase complementation assays showed interactions between Pik‐W25‐1 and the three alleles”
t0“rice blast”
Claim · model-written summary
Pik-W25-2 did not interact with the tested AvrPik alleles in the yeast assay.
- Taxon
- riceOryza sativat0
- Gene
- Pik‐W25‐2e0
- Outcome
- could not interact with AvrPik‐C, ‐D, and ‐ E allelese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pik‐W25‐2 could not interact with AvrPik‐C, ‐D, and ‐ E alleles with Y 2 H assay”
t0“rice blast”
Claim · model-written summary
The G9 line showed enhanced field resistance to leaf and panicle blast relative to CO39.
- Taxon
- riceOryza sativat0
- Material
- Pik‐W25 NILe0
- Outcome
- field resistance to leaf and panicle blaste0
- Direction
- enhancede0
- Comparator
- CO39e0
- Setting
- fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Pik‐W25 NIL exhibited enhanced field resistance to leaf and panicle blast without significant changes in morphology or development compared to the parent variety CO39”
t0“rice blast”
Claim · model-written summary
G9 showed no significant morphological or developmental difference from CO39.
- Taxon
- riceOryza sativat0
- Material
- Pik‐W25 NILe0
- Outcome
- morphology or developmente0
- Direction
- without significant changese0
- Comparator
- CO39e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Pik‐W25 NIL exhibited enhanced field resistance to leaf and panicle blast without significant changes in morphology or development compared to the parent variety CO39”
t0“rice blast”
The same questions in other papers
loss of function · disease · Oryza sativa (rice)Positive and negative results
67 papers in the same direction as this paper · 86 in the opposite direction
- positive
- positive
- positive
150 papers more in this group
See all 154 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.